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Nfic regulates tooth root patterning and growth.

Kim TH, Bae CH, Yang S, Park JC, Cho ES - Anat Cell Biol (2015)

Bottom Line: In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars.These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner.From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences, School of Dentistry, Chonbuk National University, Jeonju, Korea.

ABSTRACT
Molecular interactions between epithelium and mesenchyme are important for root formation. Nuclear factor I-C (Nfic) has been identified as a key regulator of root formation. However, the mechanisms of root formation and their interactions between Hertwig's epithelial root sheath (HERS) and mesenchyme remain unclear. In this study, we investigated the role of Nfic in root patterning and growth during molar root development. The molars of Nfic knockout mice exhibited an enlarged pulp chamber and apical displacement of the pulpal floor, characteristic features of taurodontism, due to delayed furcation formation. In developing molar roots of mutant mice at P14, BrdU positive cells decreased in the apical mesenchyme of the elongation region whereas those cells increased in the dental papilla of the furcation region. Whereas cytokeratin 14 and laminin were localized in HERS cells of mutant molars, Smoothened (Smo) and Gli1 were downregulated in preodontoblasts. In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars. These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner. From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopy of impaired root patterning in the developing mandibular first molars of nuclear factor I-C (Nfic) knockout mice. (A-G) Lateral/baso-lateral view of molar roots. Roots were gradually elongated with age in wild type (WT) mice, whereas root elongation was impaired in mutant (MT) mice. (H-N) Basal view of molar roots. In WT mice, root furcation between mesial and distal roots was regularly observed. In contrast, furcation was not observed in MT mice. At 28 days old (P28), apical displacement of furcation was observed, but no furcation was occasionally observed (25% penetrance; n=8). The white arrows indicate furcation. Scale bars=200 µm.
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Figure 1: Scanning electron microscopy of impaired root patterning in the developing mandibular first molars of nuclear factor I-C (Nfic) knockout mice. (A-G) Lateral/baso-lateral view of molar roots. Roots were gradually elongated with age in wild type (WT) mice, whereas root elongation was impaired in mutant (MT) mice. (H-N) Basal view of molar roots. In WT mice, root furcation between mesial and distal roots was regularly observed. In contrast, furcation was not observed in MT mice. At 28 days old (P28), apical displacement of furcation was observed, but no furcation was occasionally observed (25% penetrance; n=8). The white arrows indicate furcation. Scale bars=200 µm.

Mentions: To observe the morphological features of molar roots during development, we isolated the mandibular first molars of mice at P14, P18, and P28. Under observation with scanning electron microscopy, roots were gradually elongated with age in WT mice but roots were short due to disrupted elongation in MT mice (Fig. 1A-G). In examination of the basal aspect of molar roots, root furcation between mesial and distal roots was regularly observed in WT mice (Fig. 1H, J, L). However, furcation was not observed in MT mice at P14 and P18. In MT mice at P28, apically displaced incomplete furcation was observed (Fig. 1G, N). No furcation was occasionally observed in MT mice (25% penetrance; n=8).


Nfic regulates tooth root patterning and growth.

Kim TH, Bae CH, Yang S, Park JC, Cho ES - Anat Cell Biol (2015)

Scanning electron microscopy of impaired root patterning in the developing mandibular first molars of nuclear factor I-C (Nfic) knockout mice. (A-G) Lateral/baso-lateral view of molar roots. Roots were gradually elongated with age in wild type (WT) mice, whereas root elongation was impaired in mutant (MT) mice. (H-N) Basal view of molar roots. In WT mice, root furcation between mesial and distal roots was regularly observed. In contrast, furcation was not observed in MT mice. At 28 days old (P28), apical displacement of furcation was observed, but no furcation was occasionally observed (25% penetrance; n=8). The white arrows indicate furcation. Scale bars=200 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582161&req=5

Figure 1: Scanning electron microscopy of impaired root patterning in the developing mandibular first molars of nuclear factor I-C (Nfic) knockout mice. (A-G) Lateral/baso-lateral view of molar roots. Roots were gradually elongated with age in wild type (WT) mice, whereas root elongation was impaired in mutant (MT) mice. (H-N) Basal view of molar roots. In WT mice, root furcation between mesial and distal roots was regularly observed. In contrast, furcation was not observed in MT mice. At 28 days old (P28), apical displacement of furcation was observed, but no furcation was occasionally observed (25% penetrance; n=8). The white arrows indicate furcation. Scale bars=200 µm.
Mentions: To observe the morphological features of molar roots during development, we isolated the mandibular first molars of mice at P14, P18, and P28. Under observation with scanning electron microscopy, roots were gradually elongated with age in WT mice but roots were short due to disrupted elongation in MT mice (Fig. 1A-G). In examination of the basal aspect of molar roots, root furcation between mesial and distal roots was regularly observed in WT mice (Fig. 1H, J, L). However, furcation was not observed in MT mice at P14 and P18. In MT mice at P28, apically displaced incomplete furcation was observed (Fig. 1G, N). No furcation was occasionally observed in MT mice (25% penetrance; n=8).

Bottom Line: In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars.These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner.From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences, School of Dentistry, Chonbuk National University, Jeonju, Korea.

ABSTRACT
Molecular interactions between epithelium and mesenchyme are important for root formation. Nuclear factor I-C (Nfic) has been identified as a key regulator of root formation. However, the mechanisms of root formation and their interactions between Hertwig's epithelial root sheath (HERS) and mesenchyme remain unclear. In this study, we investigated the role of Nfic in root patterning and growth during molar root development. The molars of Nfic knockout mice exhibited an enlarged pulp chamber and apical displacement of the pulpal floor, characteristic features of taurodontism, due to delayed furcation formation. In developing molar roots of mutant mice at P14, BrdU positive cells decreased in the apical mesenchyme of the elongation region whereas those cells increased in the dental papilla of the furcation region. Whereas cytokeratin 14 and laminin were localized in HERS cells of mutant molars, Smoothened (Smo) and Gli1 were downregulated in preodontoblasts. In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars. These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner. From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

No MeSH data available.


Related in: MedlinePlus