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Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus

CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the penile skin. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the bottom of the coronary sulcus or groove under the penile prepuce using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F). Abundant suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains pyramidal Langerhans cells (F).
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Figure 5: CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the penile skin. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the bottom of the coronary sulcus or groove under the penile prepuce using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F). Abundant suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains pyramidal Langerhans cells (F).

Mentions: The distribution of immunoreactive cells in the penile skin was characterized by abundant suppressor lymphocytes migrating into the squamous epithelium (Fig. 5). The lymphocytes tended to be arranged along the basal layer of the epithelium. The density of suppressor lymphocytes was consistently greatest among the three cell types examined (Tables 1, 2, 3). Notably, intra-epithelial suppressor lymphocytes were pyramidal in shape, in contrast to their spherical form in the subcutaneous tissue (Fig. 5D). Observations using adjacent sections suggested that these pyramidal cells were unlikely to be double-positive for CD1a. The subcutaneous suppressor lymphocytes were more numerous at the bottom of the coronary sulci or grooves than in the skin around the sulci (Fig. 5A). Langerhans cells were also concentrated at the bottom of the sulci, and their dendritic processes were directed toward the epithelial surface (Fig. 5C, F). Macrophages in the penile skin were restricted to the subcutaneous tissue (Fig. 5B, E). The squamous epithelium at the urethral opening (i.e., the navicular fossa) also contained abundant suppressor lymphocytes and macrophages, but Langerhans cells were very sparse at the opening (figure not shown). The densities of all three cell types examined did not show a great individual difference such as that evident in the oral floor and lip.


Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the penile skin. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the bottom of the coronary sulcus or groove under the penile prepuce using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F). Abundant suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains pyramidal Langerhans cells (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582160&req=5

Figure 5: CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the penile skin. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the bottom of the coronary sulcus or groove under the penile prepuce using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F). Abundant suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains pyramidal Langerhans cells (F).
Mentions: The distribution of immunoreactive cells in the penile skin was characterized by abundant suppressor lymphocytes migrating into the squamous epithelium (Fig. 5). The lymphocytes tended to be arranged along the basal layer of the epithelium. The density of suppressor lymphocytes was consistently greatest among the three cell types examined (Tables 1, 2, 3). Notably, intra-epithelial suppressor lymphocytes were pyramidal in shape, in contrast to their spherical form in the subcutaneous tissue (Fig. 5D). Observations using adjacent sections suggested that these pyramidal cells were unlikely to be double-positive for CD1a. The subcutaneous suppressor lymphocytes were more numerous at the bottom of the coronary sulci or grooves than in the skin around the sulci (Fig. 5A). Langerhans cells were also concentrated at the bottom of the sulci, and their dendritic processes were directed toward the epithelial surface (Fig. 5C, F). Macrophages in the penile skin were restricted to the subcutaneous tissue (Fig. 5B, E). The squamous epithelium at the urethral opening (i.e., the navicular fossa) also contained abundant suppressor lymphocytes and macrophages, but Langerhans cells were very sparse at the opening (figure not shown). The densities of all three cell types examined did not show a great individual difference such as that evident in the oral floor and lip.

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus