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Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the anal canal. Immunohistochemistry of CD8 (A, D, E), CD68 (B, F, G), and CD1a (C, H). Panel (A-C) display the same mucosal groove using near sections at the same magnification (scale bar in panel A=1 mm), while panels (D), (F), and (H) correspond to square in panels (A-C) at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (D, F). Panels (E) and (G) (scale bar in panel E=0.1 mm) show submucosal tissue around a vein (corresponding to circle in panels A and B, respectively). Suppressor lymphocytes as well as macrophages are concentrated not in and along the epithelium, but near the submucosal vessels (D-G). Langerhans cells extend in parallel with (not toward) the epithelial surface (H).
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Figure 4: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the anal canal. Immunohistochemistry of CD8 (A, D, E), CD68 (B, F, G), and CD1a (C, H). Panel (A-C) display the same mucosal groove using near sections at the same magnification (scale bar in panel A=1 mm), while panels (D), (F), and (H) correspond to square in panels (A-C) at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (D, F). Panels (E) and (G) (scale bar in panel E=0.1 mm) show submucosal tissue around a vein (corresponding to circle in panels A and B, respectively). Suppressor lymphocytes as well as macrophages are concentrated not in and along the epithelium, but near the submucosal vessels (D-G). Langerhans cells extend in parallel with (not toward) the epithelial surface (H).

Mentions: The anal squamous epithelium was characterized by a low content of immunoreactive cells (Fig. 4). Among the three types of cells examined, Langerhans cells were usually th greatest in number, followed by macrophages, whereas suppressor lymphocytes were fewest (Tables 1, 2, 3, Fig. 4C). They tended to be concentrated in thick finger-like protrusions of the stratum spinosum between dermal papillae. Macrophages and lymphocytes were more richly distributed along the submucosal vessels than along the basal layer of the epithelium (Fig. 4E, G). The dendritic processes of Langerhans cells tended to extend in parallel with (not toward) the epithelial surface (Fig. 4H). In the anal canal, lymphocytes as well as macrophages were much more numerous in the columnar epithelium than in the squamous epithelium (figure not shown). The densities of all three cell types examined did not show great individual differences.


Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the anal canal. Immunohistochemistry of CD8 (A, D, E), CD68 (B, F, G), and CD1a (C, H). Panel (A-C) display the same mucosal groove using near sections at the same magnification (scale bar in panel A=1 mm), while panels (D), (F), and (H) correspond to square in panels (A-C) at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (D, F). Panels (E) and (G) (scale bar in panel E=0.1 mm) show submucosal tissue around a vein (corresponding to circle in panels A and B, respectively). Suppressor lymphocytes as well as macrophages are concentrated not in and along the epithelium, but near the submucosal vessels (D-G). Langerhans cells extend in parallel with (not toward) the epithelial surface (H).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582160&req=5

Figure 4: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the anal canal. Immunohistochemistry of CD8 (A, D, E), CD68 (B, F, G), and CD1a (C, H). Panel (A-C) display the same mucosal groove using near sections at the same magnification (scale bar in panel A=1 mm), while panels (D), (F), and (H) correspond to square in panels (A-C) at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (D, F). Panels (E) and (G) (scale bar in panel E=0.1 mm) show submucosal tissue around a vein (corresponding to circle in panels A and B, respectively). Suppressor lymphocytes as well as macrophages are concentrated not in and along the epithelium, but near the submucosal vessels (D-G). Langerhans cells extend in parallel with (not toward) the epithelial surface (H).
Mentions: The anal squamous epithelium was characterized by a low content of immunoreactive cells (Fig. 4). Among the three types of cells examined, Langerhans cells were usually th greatest in number, followed by macrophages, whereas suppressor lymphocytes were fewest (Tables 1, 2, 3, Fig. 4C). They tended to be concentrated in thick finger-like protrusions of the stratum spinosum between dermal papillae. Macrophages and lymphocytes were more richly distributed along the submucosal vessels than along the basal layer of the epithelium (Fig. 4E, G). The dendritic processes of Langerhans cells tended to extend in parallel with (not toward) the epithelial surface (Fig. 4H). In the anal canal, lymphocytes as well as macrophages were much more numerous in the columnar epithelium than in the squamous epithelium (figure not shown). The densities of all three cell types examined did not show great individual differences.

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus