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Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the palpebral conjunctiva. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal grooves using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not have maximum density (D, E). A few suppressor lymphocytes and macrophages have migrated into the epithelium (arrows in panels D and E). The epithelium contains abundant pyramidal or dendritic Langerhans cells (F).
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Figure 3: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the palpebral conjunctiva. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal grooves using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not have maximum density (D, E). A few suppressor lymphocytes and macrophages have migrated into the epithelium (arrows in panels D and E). The epithelium contains abundant pyramidal or dendritic Langerhans cells (F).

Mentions: At a lower magnification, the palpebral conjunctiva usually exhibited a diffuse and dense distribution of the cells examined (Fig. 3). Langerhans cells were consistently less numerous than suppressor lymphocytes or macrophages (Tables 1, 2, 3). Especially in the upper eyelid, the conjunctiva usually contained abundant Langerhans cells with highly developed dendritic processes toward the epithelial surface (Fig. 3C, F). They were evenly distributed in the thin stratum spinosum of the squamous epithelium. In contrast, far fewer immunoreactive cells were seen in the bulbar conjunctiva (figure not shown). Short grooves of the palpebral conjunctiva tended to be covered by immunereactive cell-poor epithelium (Fig. 3A, B). In the conjunctiva, the highest cell density was usually seen at the attachment site between the upper and lower eyelids (Fig. 3A-C). Suppressor lymphocytes and/or macrophages were sometimes seen to have migrated into the squamous epithelium (Fig. 3D, E). The density of suppressor lymphocytes varied more than 5-fold between individuals, but macrophages did not show such a large difference (Tables 2, 3).


Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the palpebral conjunctiva. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal grooves using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not have maximum density (D, E). A few suppressor lymphocytes and macrophages have migrated into the epithelium (arrows in panels D and E). The epithelium contains abundant pyramidal or dendritic Langerhans cells (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582160&req=5

Figure 3: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the palpebral conjunctiva. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal grooves using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not have maximum density (D, E). A few suppressor lymphocytes and macrophages have migrated into the epithelium (arrows in panels D and E). The epithelium contains abundant pyramidal or dendritic Langerhans cells (F).
Mentions: At a lower magnification, the palpebral conjunctiva usually exhibited a diffuse and dense distribution of the cells examined (Fig. 3). Langerhans cells were consistently less numerous than suppressor lymphocytes or macrophages (Tables 1, 2, 3). Especially in the upper eyelid, the conjunctiva usually contained abundant Langerhans cells with highly developed dendritic processes toward the epithelial surface (Fig. 3C, F). They were evenly distributed in the thin stratum spinosum of the squamous epithelium. In contrast, far fewer immunoreactive cells were seen in the bulbar conjunctiva (figure not shown). Short grooves of the palpebral conjunctiva tended to be covered by immunereactive cell-poor epithelium (Fig. 3A, B). In the conjunctiva, the highest cell density was usually seen at the attachment site between the upper and lower eyelids (Fig. 3A-C). Suppressor lymphocytes and/or macrophages were sometimes seen to have migrated into the squamous epithelium (Fig. 3D, E). The density of suppressor lymphocytes varied more than 5-fold between individuals, but macrophages did not show such a large difference (Tables 2, 3).

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus