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Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus

CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the lower lip. Immunohistochemistry of CD8 (A, E), CD68 (B, F), and CD1a (C, D). Panels (A-C) display the same site using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F).
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Figure 2: CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the lower lip. Immunohistochemistry of CD8 (A, E), CD68 (B, F), and CD1a (C, D). Panels (A-C) display the same site using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F).

Mentions: The lip of the mouth was also characterized by a rich content of immunoreactive cells (Fig. 2) despite the fact that the epithelial thickness (0.1-0.2 mm) was almost half that of the oral mucosa. Langerhans cells, most of which were highly dendritic, tended to be concentrated along the papilla. Langerhans cell density in the lip showed more than a 10-fold individual difference (Table 1). Suppressor lymphocytes always and macrophages sometimes were seen to have migrated into the epithelium, especially along the papilla. These cells were also richly distributed along thin subcutaneous vessels. Suppressor lymphocytes tended to be arranged along the basal layer of the epithelium. Cell-cell junctions in the stratum spinosum of the epithelium sometimes expressed CD8 reactivity (figure not shown). Suppressor lymphocytes were consistently fewer in number than on the oral floor of the same specimen. The density of suppressor lymphocytes showed more than a 5-fold difference between individuals, but macrophages did not exhibit such a great difference (Tables 2, 3).


Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the lower lip. Immunohistochemistry of CD8 (A, E), CD68 (B, F), and CD1a (C, D). Panels (A-C) display the same site using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582160&req=5

Figure 2: CD1a-positive Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages in the lower lip. Immunohistochemistry of CD8 (A, E), CD68 (B, F), and CD1a (C, D). Panels (A-C) display the same site using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density (E, F).
Mentions: The lip of the mouth was also characterized by a rich content of immunoreactive cells (Fig. 2) despite the fact that the epithelial thickness (0.1-0.2 mm) was almost half that of the oral mucosa. Langerhans cells, most of which were highly dendritic, tended to be concentrated along the papilla. Langerhans cell density in the lip showed more than a 10-fold individual difference (Table 1). Suppressor lymphocytes always and macrophages sometimes were seen to have migrated into the epithelium, especially along the papilla. These cells were also richly distributed along thin subcutaneous vessels. Suppressor lymphocytes tended to be arranged along the basal layer of the epithelium. Cell-cell junctions in the stratum spinosum of the epithelium sometimes expressed CD8 reactivity (figure not shown). Suppressor lymphocytes were consistently fewer in number than on the oral floor of the same specimen. The density of suppressor lymphocytes showed more than a 5-fold difference between individuals, but macrophages did not exhibit such a great difference (Tables 2, 3).

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus