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Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the oral floor mucosa. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal fold using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density in panels (E) and (F). Some of suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains abundant spherical Langerhans cells (arrows in panel F).
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Figure 1: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the oral floor mucosa. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal fold using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density in panels (E) and (F). Some of suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains abundant spherical Langerhans cells (arrows in panel F).

Mentions: The density of positive cells (number per visual field) was measured by manual counting under a ×20 objective lens. The field corresponding to a circle 0.8 mm in diameter contained an almost 1-mm surface length of slightly curved epithelium. For measurement, we chose one best section (clearly stained) from each of the three types of immunostainings of an individual structure. As CD8-positive cells and/or CS68-positive cells were often restricted to the submucosal or subcutaneous tissue, we also counted these cells in tissue within 0.2 mm from the base of the epithelium. This counting was performed at a hot spot (a site with the highest density) in each section: this is a routine method for evaluation of metastatic lymph vessels in specimens obtained surgically from cancer patients [9]. However, for convenience of comparison, we prepared the present figures at a single hot spot for one of the 3 cell types examined, such as for CD8-positive cells in Fig. 1. Statistical analyses were carried out using PASW version 18 (SPSS Inc., Chicago, IL, USA). The cell density data were analyzed using Tukey's test (Tables 4, 5, 6).


Regional differences in the density of Langerhans cells, CD8-positive T lymphocytes and CD68-positive macrophages: a preliminary study using elderly donated cadavers.

Omine Y, Hinata N, Yamamoto M, Kasahara M, Matsunaga S, Murakami G, Abe S - Anat Cell Biol (2015)

CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the oral floor mucosa. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal fold using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density in panels (E) and (F). Some of suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains abundant spherical Langerhans cells (arrows in panel F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582160&req=5

Figure 1: CD1a-positive Langerhans cells, CD8-positive T lymphocytes, and CD68-positive macrophages in the oral floor mucosa. Immunohistochemistry of CD8 (A, D), CD68 (B, E), and CD1a (C, F). Panel (A-C) display the same mucosal fold using adjacent sections at the same magnification (scale bar in panel A=1 mm), while panels (D-F) correspond to square in panels (A-C), respectively at the same magnification (scale bar in panel D=0.1 mm). Thus, the positive cells do not show maximum density in panels (E) and (F). Some of suppressor lymphocytes have migrated into the epithelium (D), in contrast to macrophages (E). The epithelium contains abundant spherical Langerhans cells (arrows in panel F).
Mentions: The density of positive cells (number per visual field) was measured by manual counting under a ×20 objective lens. The field corresponding to a circle 0.8 mm in diameter contained an almost 1-mm surface length of slightly curved epithelium. For measurement, we chose one best section (clearly stained) from each of the three types of immunostainings of an individual structure. As CD8-positive cells and/or CS68-positive cells were often restricted to the submucosal or subcutaneous tissue, we also counted these cells in tissue within 0.2 mm from the base of the epithelium. This counting was performed at a hot spot (a site with the highest density) in each section: this is a routine method for evaluation of metastatic lymph vessels in specimens obtained surgically from cancer patients [9]. However, for convenience of comparison, we prepared the present figures at a single hot spot for one of the 3 cell types examined, such as for CD8-positive cells in Fig. 1. Statistical analyses were carried out using PASW version 18 (SPSS Inc., Chicago, IL, USA). The cell density data were analyzed using Tukey's test (Tables 4, 5, 6).

Bottom Line: In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens.In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined.The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Tokyo Dental College, Tokyo, Japan.

ABSTRACT
To provide a better understanding of the local immune system in the face and external genitalia, i.e., the oral floor, lower lip, palpebral conjunctiva, anus and penis, we examined the distribution and density of CD1a-positve Langerhans cells, CD8-positive suppressor T lymphocytes and CD68-positive macrophages using specimens from 8 male elderly cadavers. The density of Langerhans cells showed an individual difference of more than (or almost) 10-fold in the lip (oral floor). In the oral floor, Langerhans cells were often spherical. Submucosal or subcutaneous suppressor lymphocytes, especially rich in the oral floor and penile skin, migrated into the epithelium at 4 sites, except for the anus. In the conjunctiva, macrophage migration into the epithelium was seen in all 8 specimens. The density of suppressor lymphocytes showed a significant correlation between the oral floor and the lip (r=0.78). In contrast, the anal and penile skins showed no positive correlation in the density of all three types of immunoreactive cells examined. Overall, irrespective of the wide individual differences, the oral floor and conjunctiva seemed to be characterized by a rich content of all three cell types, whereas the penile skin was characterized by an abundance of suppressor lymphocytes. Based on the tables, as mean value, the relative abundance of three different cell types were as follows; CD1a-positive Langerhans cells (anus), CD8-positive lymphocytes (penis), and CD68-positive macrophages (lip). The present observations suggest that the local immune response is highly site-dependent, with a tendency for tolerance rather than rejection.

No MeSH data available.


Related in: MedlinePlus