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Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus

Quantitative analysis of cells expressing glial fibrillary acidic protein (GFAP) (A) and S100 (B) in both fetal bovine serum (FBS)- and human serum (HS)-treated groups (data are mean cells±SEM, %). *P<0.05.
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Figure 5: Quantitative analysis of cells expressing glial fibrillary acidic protein (GFAP) (A) and S100 (B) in both fetal bovine serum (FBS)- and human serum (HS)-treated groups (data are mean cells±SEM, %). *P<0.05.

Mentions: To compare the effects of two different serums on the differentiation capacity of ASCs into SC-like cells, the number of SC-like cells was counted using two markers in both groups. Quantitative analysis indicated that 2.3±0.0% of the cells expressed GFAP in HS-treated groups while 11.6±1.0% expressed the same marker in FBS-treated groups (Fig. 5). Furthermore, S100-positive cells were 2.6±1.0% and 14.0±2.08% in HS-treated and FBS-treated groups, respectively.


Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Quantitative analysis of cells expressing glial fibrillary acidic protein (GFAP) (A) and S100 (B) in both fetal bovine serum (FBS)- and human serum (HS)-treated groups (data are mean cells±SEM, %). *P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582159&req=5

Figure 5: Quantitative analysis of cells expressing glial fibrillary acidic protein (GFAP) (A) and S100 (B) in both fetal bovine serum (FBS)- and human serum (HS)-treated groups (data are mean cells±SEM, %). *P<0.05.
Mentions: To compare the effects of two different serums on the differentiation capacity of ASCs into SC-like cells, the number of SC-like cells was counted using two markers in both groups. Quantitative analysis indicated that 2.3±0.0% of the cells expressed GFAP in HS-treated groups while 11.6±1.0% expressed the same marker in FBS-treated groups (Fig. 5). Furthermore, S100-positive cells were 2.6±1.0% and 14.0±2.08% in HS-treated and FBS-treated groups, respectively.

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus