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Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence staining indicated that the differentiated Schwann-like cells expressed S100 (A, C) and glial fibrillary acidic protein (GFAP) (B, D) in both human serum (HS)- and fetal bovine serum (FBS)-treated groups. Fibroblasts 3T3 and undifferentiated adipose-derived stem cells were taken as negative control and new born rat Schwann cells was considered as positive control. Scale bars=30 mm (A-D).
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Figure 4: Immunofluorescence staining indicated that the differentiated Schwann-like cells expressed S100 (A, C) and glial fibrillary acidic protein (GFAP) (B, D) in both human serum (HS)- and fetal bovine serum (FBS)-treated groups. Fibroblasts 3T3 and undifferentiated adipose-derived stem cells were taken as negative control and new born rat Schwann cells was considered as positive control. Scale bars=30 mm (A-D).

Mentions: None of SCs-related proteins was detected in undifferentiated ASCs (data not shown) but both were expressed in differentiated ASCs treated by both FBS and HS (Fig. 4).


Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Immunofluorescence staining indicated that the differentiated Schwann-like cells expressed S100 (A, C) and glial fibrillary acidic protein (GFAP) (B, D) in both human serum (HS)- and fetal bovine serum (FBS)-treated groups. Fibroblasts 3T3 and undifferentiated adipose-derived stem cells were taken as negative control and new born rat Schwann cells was considered as positive control. Scale bars=30 mm (A-D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582159&req=5

Figure 4: Immunofluorescence staining indicated that the differentiated Schwann-like cells expressed S100 (A, C) and glial fibrillary acidic protein (GFAP) (B, D) in both human serum (HS)- and fetal bovine serum (FBS)-treated groups. Fibroblasts 3T3 and undifferentiated adipose-derived stem cells were taken as negative control and new born rat Schwann cells was considered as positive control. Scale bars=30 mm (A-D).
Mentions: None of SCs-related proteins was detected in undifferentiated ASCs (data not shown) but both were expressed in differentiated ASCs treated by both FBS and HS (Fig. 4).

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus