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Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus

Morphological assessment of Schwann-like (SC-like) cells after 14 days of induction. (A) Adipose-derived stem cells at fourth passage on day 0 of induction by β-mercaptoethanol. (B) SC-like cells with deformed morphologies in human serum (HS)-treated groups. Most of cells detached in response to the differentiation medium containing allogeneic HS. (C) SC-like cells appeared mostly bipolar or tripolar, typical morphology of Schwann cells when exposed to the differentiation medium containing fetal bovine serum. Scale bars=40 µm (A-C).
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Figure 3: Morphological assessment of Schwann-like (SC-like) cells after 14 days of induction. (A) Adipose-derived stem cells at fourth passage on day 0 of induction by β-mercaptoethanol. (B) SC-like cells with deformed morphologies in human serum (HS)-treated groups. Most of cells detached in response to the differentiation medium containing allogeneic HS. (C) SC-like cells appeared mostly bipolar or tripolar, typical morphology of Schwann cells when exposed to the differentiation medium containing fetal bovine serum. Scale bars=40 µm (A-C).

Mentions: hASCs at passage 4 were exposed to the glial growth factors for 2 weeks. At the end, morphology of SC-like cells and the expression of two SCs-related proteins, GFAP and S100, were evaluated. After beginning the differentiation, some cells started to change their morphology and adopted a bipolar or tripolar morphologies which were similar to SCs in both groups. Interestingly, as it was assessed qualitatively, many cells seemed to be detached in HS-treated groups after adding differentiation medium while the attached differentiated cells displayed a deformed shape, indicative of being under stress. In contrast, these events were less evident in FBS-treated groups (Fig. 3). Cell counting at the end of differentiation period also confirmed this matter and indicated that there are more cells left in wells containing FBS (9×103 cells/well) as statistically compared to wells containing HS (3×103 cells/well) (P<0.001).


Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Morphological assessment of Schwann-like (SC-like) cells after 14 days of induction. (A) Adipose-derived stem cells at fourth passage on day 0 of induction by β-mercaptoethanol. (B) SC-like cells with deformed morphologies in human serum (HS)-treated groups. Most of cells detached in response to the differentiation medium containing allogeneic HS. (C) SC-like cells appeared mostly bipolar or tripolar, typical morphology of Schwann cells when exposed to the differentiation medium containing fetal bovine serum. Scale bars=40 µm (A-C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582159&req=5

Figure 3: Morphological assessment of Schwann-like (SC-like) cells after 14 days of induction. (A) Adipose-derived stem cells at fourth passage on day 0 of induction by β-mercaptoethanol. (B) SC-like cells with deformed morphologies in human serum (HS)-treated groups. Most of cells detached in response to the differentiation medium containing allogeneic HS. (C) SC-like cells appeared mostly bipolar or tripolar, typical morphology of Schwann cells when exposed to the differentiation medium containing fetal bovine serum. Scale bars=40 µm (A-C).
Mentions: hASCs at passage 4 were exposed to the glial growth factors for 2 weeks. At the end, morphology of SC-like cells and the expression of two SCs-related proteins, GFAP and S100, were evaluated. After beginning the differentiation, some cells started to change their morphology and adopted a bipolar or tripolar morphologies which were similar to SCs in both groups. Interestingly, as it was assessed qualitatively, many cells seemed to be detached in HS-treated groups after adding differentiation medium while the attached differentiated cells displayed a deformed shape, indicative of being under stress. In contrast, these events were less evident in FBS-treated groups (Fig. 3). Cell counting at the end of differentiation period also confirmed this matter and indicated that there are more cells left in wells containing FBS (9×103 cells/well) as statistically compared to wells containing HS (3×103 cells/well) (P<0.001).

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus