Limits...
Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus

Differentiation potential assay of adipose-derived stem cells (ASCs) at fourth passage. (A) Lipid vacuoles inside the cytoplasm of adipoblasts appear as red circular spots after staining with oil Red O which stains triglyceride and neutral lipid. (B) Calcium deposition was shown by alizarin red S staining as dark red spots in the plate. Scale bars=30 µm (A, B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4582159&req=5

Figure 2: Differentiation potential assay of adipose-derived stem cells (ASCs) at fourth passage. (A) Lipid vacuoles inside the cytoplasm of adipoblasts appear as red circular spots after staining with oil Red O which stains triglyceride and neutral lipid. (B) Calcium deposition was shown by alizarin red S staining as dark red spots in the plate. Scale bars=30 µm (A, B).

Mentions: Most of the hASCs expressed high levels of CD44 (99.97%), CD29-integrin β1 (98.97%), and CD90 Thy-1 (99.73%) but they were almost negative for CD45 (0.57%). The plasticity of hASCs was also assessed by induction of adipogenic and osteogenic differentiations and was demonstrated by lipid vacuoles and calcium deposits, respectively (Fig. 2). These results confirmed the mesenchymal nature of the isolated cells as well as their multipotentiality.


Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Differentiation potential assay of adipose-derived stem cells (ASCs) at fourth passage. (A) Lipid vacuoles inside the cytoplasm of adipoblasts appear as red circular spots after staining with oil Red O which stains triglyceride and neutral lipid. (B) Calcium deposition was shown by alizarin red S staining as dark red spots in the plate. Scale bars=30 µm (A, B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582159&req=5

Figure 2: Differentiation potential assay of adipose-derived stem cells (ASCs) at fourth passage. (A) Lipid vacuoles inside the cytoplasm of adipoblasts appear as red circular spots after staining with oil Red O which stains triglyceride and neutral lipid. (B) Calcium deposition was shown by alizarin red S staining as dark red spots in the plate. Scale bars=30 µm (A, B).
Mentions: Most of the hASCs expressed high levels of CD44 (99.97%), CD29-integrin β1 (98.97%), and CD90 Thy-1 (99.73%) but they were almost negative for CD45 (0.57%). The plasticity of hASCs was also assessed by induction of adipogenic and osteogenic differentiations and was demonstrated by lipid vacuoles and calcium deposits, respectively (Fig. 2). These results confirmed the mesenchymal nature of the isolated cells as well as their multipotentiality.

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus