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Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus

Adipose-derived stem cells (ASCs) isolation and culture. (A) Cultured cells of stromal vascular fraction of fat tissue on day 3 of primary culture. (B) ASCs at passage 2 at confluence. Scale bars=30 µm (A, B).
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Figure 1: Adipose-derived stem cells (ASCs) isolation and culture. (A) Cultured cells of stromal vascular fraction of fat tissue on day 3 of primary culture. (B) ASCs at passage 2 at confluence. Scale bars=30 µm (A, B).

Mentions: After the cells adhered to the tissue culture flask, non-adherent cells, such as red blood cells, were removed by changing the culture medium after 2 days. The initially adherent cells grew into spindle or stellate-shaped cells, which then developed into visible colonies 3 days after the initial plating (Fig. 1A). The cells began to proliferate rapidly and were passaged by trypsination every 3-4 days until they were completely confluent. After the second passage, hASCs appeared to adopt a more uniform fibroblast-like shape with regular directions (Fig. 1B).


Differentiation of adipose-derived stem cells into Schwann-like cells: fetal bovine serum or human serum?

Younesi E, Bayati V, Hashemitabar M, Azandeh SS, Bijannejad D, Bahreini A - Anat Cell Biol (2015)

Adipose-derived stem cells (ASCs) isolation and culture. (A) Cultured cells of stromal vascular fraction of fat tissue on day 3 of primary culture. (B) ASCs at passage 2 at confluence. Scale bars=30 µm (A, B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582159&req=5

Figure 1: Adipose-derived stem cells (ASCs) isolation and culture. (A) Cultured cells of stromal vascular fraction of fat tissue on day 3 of primary culture. (B) ASCs at passage 2 at confluence. Scale bars=30 µm (A, B).
Mentions: After the cells adhered to the tissue culture flask, non-adherent cells, such as red blood cells, were removed by changing the culture medium after 2 days. The initially adherent cells grew into spindle or stellate-shaped cells, which then developed into visible colonies 3 days after the initial plating (Fig. 1A). The cells began to proliferate rapidly and were passaged by trypsination every 3-4 days until they were completely confluent. After the second passage, hASCs appeared to adopt a more uniform fibroblast-like shape with regular directions (Fig. 1B).

Bottom Line: The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research.On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05).It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
Access to autologous Schwann cells is limited due to lack of donor site and its difficult isolation and culture. Therefore, one of the possible ways to obtain to Schwann cells is to differentiate mesenchymal stem cells into glial pathway using various materials and protocols. The aim of this study was to compare the effects of fetal bovine serum and human serum on Schwann cell differentiation of adipose-derived stem cells to choose the best serum for use in future research. For this purpose, after isolation of human adipose-derived stem cells, it was characterized and differentiated into Schwann cell lineage using two protocols which one of them contained fetal bovine serum and the other human serum. At the end, morphological evaluation declared an increased detachment of cells in response to human serum. On the other side, immunocytochemistry showed that there was a significant increase in the number of cells expressing glial fibrillary acidic proteins and S100 in fetal bovine serum-treated group when compared to human serum-treated one (P<0.05). It was concluded that fetal bovine serum was more effective than allogeneic human serum in Schwann cell differentiation of adipose-derived stem cells.

No MeSH data available.


Related in: MedlinePlus