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Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase mice: a serendipitous model of the methylfolate trap.

Jiang H, Hurt KJ, Breen K, Stabler SP, Allen RH, Orlicky DJ, Maclean KN - Biol Open (2015)

Bottom Line: In addition to its role in the endogenous synthesis of cysteine, cystathionine gamma-lyase (CGL) is a major physiological source of the vasorelaxant hydrogen sulfide.Cgl mice of both sexes exhibited decreased expression of methylenetetrahydrofolate reductase and cysteinesulfinate decarboxylase compared to WT controls.Female Cgl mice exhibited a sex-specific induction of betaine homocysteine S-methyltransferase and methionine adenosyltransferase 1, alpha and a 70% decrease in methionine synthase expression accompanied by significantly decreased plasma methionine.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.

No MeSH data available.


Related in: MedlinePlus

MAT1A protein levels are significantly increased in female Cgl  mice with severely elevated plasma tHcy. Western blotting analysis of hepatic GNMT and MAT1A protein expression levels in male and female WT and Cgl  mice. Values shown represent the mean±s.d., *P<0.05, **P<0.01.
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BIO013433F3: MAT1A protein levels are significantly increased in female Cgl mice with severely elevated plasma tHcy. Western blotting analysis of hepatic GNMT and MAT1A protein expression levels in male and female WT and Cgl mice. Values shown represent the mean±s.d., *P<0.05, **P<0.01.

Mentions: Expression of S-adenosylhomocysteine hydrolase (SAHH) in male Cgl mice was 40% lower compared to male WT controls (P=0.021) but was unaffected in female Cgl mice (Fig. 2). Conversely, MAT1A was not significantly changed compared to controls in male Cgl mice but was increased by approximately 2.5-fold in Cgl female mice (P=0.0032) (Fig. 3). Interestingly, GNMT was significantly induced albeit at a relatively modest level, in both male and female Cgl mice (P=0.0168 and 0.0108 respectively) (Fig. 3).Fig. 3.


Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase mice: a serendipitous model of the methylfolate trap.

Jiang H, Hurt KJ, Breen K, Stabler SP, Allen RH, Orlicky DJ, Maclean KN - Biol Open (2015)

MAT1A protein levels are significantly increased in female Cgl  mice with severely elevated plasma tHcy. Western blotting analysis of hepatic GNMT and MAT1A protein expression levels in male and female WT and Cgl  mice. Values shown represent the mean±s.d., *P<0.05, **P<0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582125&req=5

BIO013433F3: MAT1A protein levels are significantly increased in female Cgl mice with severely elevated plasma tHcy. Western blotting analysis of hepatic GNMT and MAT1A protein expression levels in male and female WT and Cgl mice. Values shown represent the mean±s.d., *P<0.05, **P<0.01.
Mentions: Expression of S-adenosylhomocysteine hydrolase (SAHH) in male Cgl mice was 40% lower compared to male WT controls (P=0.021) but was unaffected in female Cgl mice (Fig. 2). Conversely, MAT1A was not significantly changed compared to controls in male Cgl mice but was increased by approximately 2.5-fold in Cgl female mice (P=0.0032) (Fig. 3). Interestingly, GNMT was significantly induced albeit at a relatively modest level, in both male and female Cgl mice (P=0.0168 and 0.0108 respectively) (Fig. 3).Fig. 3.

Bottom Line: In addition to its role in the endogenous synthesis of cysteine, cystathionine gamma-lyase (CGL) is a major physiological source of the vasorelaxant hydrogen sulfide.Cgl mice of both sexes exhibited decreased expression of methylenetetrahydrofolate reductase and cysteinesulfinate decarboxylase compared to WT controls.Female Cgl mice exhibited a sex-specific induction of betaine homocysteine S-methyltransferase and methionine adenosyltransferase 1, alpha and a 70% decrease in methionine synthase expression accompanied by significantly decreased plasma methionine.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.

No MeSH data available.


Related in: MedlinePlus