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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Functions of the BX-C genes in patterning the CCAP-ENs. (A) Summary scheme of CCAP/Bursα and HOX gene expression in the CCAP-ENs of stage 10 embryos, larvae 1 and late larvae3/early pupae. The different levels of expression of HOX genes in different segments are not represented. (B) Table summarizing the roles of Ubx, Abd-A and Abd-B in patterning CCAP-ENs in embryo and larva.
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BIO012872F7: Functions of the BX-C genes in patterning the CCAP-ENs. (A) Summary scheme of CCAP/Bursα and HOX gene expression in the CCAP-ENs of stage 10 embryos, larvae 1 and late larvae3/early pupae. The different levels of expression of HOX genes in different segments are not represented. (B) Table summarizing the roles of Ubx, Abd-A and Abd-B in patterning CCAP-ENs in embryo and larva.

Mentions: The results presented here permit several conclusions about the role of the BX-C genes in patterning the CCAP neurons: first, the only function of Ubx and Abd-A seems to be to prevent apoptosis of the CCAP-ENs in segments T3–A7 (Fig. 7). Once this early stage is passed, these gene products do not seem to be required for the maintenance of these neurons. Secondly, unlike what happens with Ubx, the level of Abd-A expression is determinant, since a high level of expression changes the fate of both CCAP-INs and -ENs. Third, unlike Ubx and Abd-A, which define the spatial pattern of CCAP-ENs, Abd-B controls the temporal onset of neuropeptide expression, such that the latter is not activated till either Abd-B is removed or its function is counteracted by activation of the ecdysone pathway. Further experiments are required to establish how this happens at the molecular level.Fig. 7.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Functions of the BX-C genes in patterning the CCAP-ENs. (A) Summary scheme of CCAP/Bursα and HOX gene expression in the CCAP-ENs of stage 10 embryos, larvae 1 and late larvae3/early pupae. The different levels of expression of HOX genes in different segments are not represented. (B) Table summarizing the roles of Ubx, Abd-A and Abd-B in patterning CCAP-ENs in embryo and larva.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F7: Functions of the BX-C genes in patterning the CCAP-ENs. (A) Summary scheme of CCAP/Bursα and HOX gene expression in the CCAP-ENs of stage 10 embryos, larvae 1 and late larvae3/early pupae. The different levels of expression of HOX genes in different segments are not represented. (B) Table summarizing the roles of Ubx, Abd-A and Abd-B in patterning CCAP-ENs in embryo and larva.
Mentions: The results presented here permit several conclusions about the role of the BX-C genes in patterning the CCAP neurons: first, the only function of Ubx and Abd-A seems to be to prevent apoptosis of the CCAP-ENs in segments T3–A7 (Fig. 7). Once this early stage is passed, these gene products do not seem to be required for the maintenance of these neurons. Secondly, unlike what happens with Ubx, the level of Abd-A expression is determinant, since a high level of expression changes the fate of both CCAP-INs and -ENs. Third, unlike Ubx and Abd-A, which define the spatial pattern of CCAP-ENs, Abd-B controls the temporal onset of neuropeptide expression, such that the latter is not activated till either Abd-B is removed or its function is counteracted by activation of the ecdysone pathway. Further experiments are required to establish how this happens at the molecular level.Fig. 7.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.