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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus

Effects of the ecdysone pathway on the expression of CCAP and Bursα. (A,A′) Expression of Dac (green) and CCAP (red) in ftz-f1Df(3L)BSC844 first instar larvae. The red channel is shown separately in grey (A′). CCAP expression is weaker. White bars indicate subesophagus/thorax/abdomen boundaries. (B) Expression of Abd-B (green) and Bursα (red) in the A6 segment of an hs-ftz-f1 early second instar larva heat shocked in late L1. An extra CCAP neuron expresses Abd-B (arrowhead). (C-D′) Expression of Bursα (red), Dac (green in C,C′) and Abd-B (green in D,D′) in the indicated abdominal segments of early larva 2 ganglia, incubated in an ETH solution and stained 4 h later.
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BIO012872F6: Effects of the ecdysone pathway on the expression of CCAP and Bursα. (A,A′) Expression of Dac (green) and CCAP (red) in ftz-f1Df(3L)BSC844 first instar larvae. The red channel is shown separately in grey (A′). CCAP expression is weaker. White bars indicate subesophagus/thorax/abdomen boundaries. (B) Expression of Abd-B (green) and Bursα (red) in the A6 segment of an hs-ftz-f1 early second instar larva heat shocked in late L1. An extra CCAP neuron expresses Abd-B (arrowhead). (C-D′) Expression of Bursα (red), Dac (green in C,C′) and Abd-B (green in D,D′) in the indicated abdominal segments of early larva 2 ganglia, incubated in an ETH solution and stained 4 h later.

Mentions: This observation supports the idea that, in normal development, the ecdysone pulse in late larvae/early pupae triggers the terminal differentiation of CCAP neurons (Richards, 1981). If so, activation of Ftz-f1 may counteract repression of CCAP by Abd-B. We stained Bursα in larvae carrying different loss-of-function alleles of ftz-f1 (ftz-f1ex19, ftz-f1ex7 and ftz-f1Df(3L)BSC844) and observed that expression, although not absent, was very weak, mostly in abdominal segments (Fig. 6A-A′ and data not shown). We also reproduced the result obtained by the misexpression of ftz-f1 (hs-ftz-f1 larva aged from 12 to 36 h. after hatching were heat shocked at 37°C during 1 h. and dissected and stained 4 h. later), and occasionally observed extra CCAP-ENs, a phenotype not observed in wild-type larvae (Fig. 6B-B′).Fig. 6.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Effects of the ecdysone pathway on the expression of CCAP and Bursα. (A,A′) Expression of Dac (green) and CCAP (red) in ftz-f1Df(3L)BSC844 first instar larvae. The red channel is shown separately in grey (A′). CCAP expression is weaker. White bars indicate subesophagus/thorax/abdomen boundaries. (B) Expression of Abd-B (green) and Bursα (red) in the A6 segment of an hs-ftz-f1 early second instar larva heat shocked in late L1. An extra CCAP neuron expresses Abd-B (arrowhead). (C-D′) Expression of Bursα (red), Dac (green in C,C′) and Abd-B (green in D,D′) in the indicated abdominal segments of early larva 2 ganglia, incubated in an ETH solution and stained 4 h later.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F6: Effects of the ecdysone pathway on the expression of CCAP and Bursα. (A,A′) Expression of Dac (green) and CCAP (red) in ftz-f1Df(3L)BSC844 first instar larvae. The red channel is shown separately in grey (A′). CCAP expression is weaker. White bars indicate subesophagus/thorax/abdomen boundaries. (B) Expression of Abd-B (green) and Bursα (red) in the A6 segment of an hs-ftz-f1 early second instar larva heat shocked in late L1. An extra CCAP neuron expresses Abd-B (arrowhead). (C-D′) Expression of Bursα (red), Dac (green in C,C′) and Abd-B (green in D,D′) in the indicated abdominal segments of early larva 2 ganglia, incubated in an ETH solution and stained 4 h later.
Mentions: This observation supports the idea that, in normal development, the ecdysone pulse in late larvae/early pupae triggers the terminal differentiation of CCAP neurons (Richards, 1981). If so, activation of Ftz-f1 may counteract repression of CCAP by Abd-B. We stained Bursα in larvae carrying different loss-of-function alleles of ftz-f1 (ftz-f1ex19, ftz-f1ex7 and ftz-f1Df(3L)BSC844) and observed that expression, although not absent, was very weak, mostly in abdominal segments (Fig. 6A-A′ and data not shown). We also reproduced the result obtained by the misexpression of ftz-f1 (hs-ftz-f1 larva aged from 12 to 36 h. after hatching were heat shocked at 37°C during 1 h. and dissected and stained 4 h. later), and occasionally observed extra CCAP-ENs, a phenotype not observed in wild-type larvae (Fig. 6B-B′).Fig. 6.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus