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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus

Abd-B represses the expression of CCAP and Bursα. (A,B) Expression of Dac (green) and Bursα (red) in Abd-BM1 ganglia of larvae 1. Higher magnification views of Bursα expression in abdominal segments (A′) and in segments A6–7 (B) are shown in white. The red bar indicates the thorax/abdomen separation. Note in B the presence of two neurons per hemisegment and the efferent axons (red arrowheads). (C) Expression of Bursα (green), CCAP (red) and Dac (blue) in elav-Gal4 UAS-Abd-B-RNAi UAS-dicer2 larvae 2. A higher magnification view of segment A7 (arrowhead) is shown on the right. (D) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-Abd-BM2. Bursα expression is absent in both INs and ENs. (E) CCAP expression in Df(3L)H99 elav-Gal4 UAS-Abd-BM2. (F) Expression of GFP (green) and CCAP (red) in CCAP-Gal4 UAS-GFP UAS-Abd-BM2 in larva 3. A higher magnification view of the boxed area is shown on the right. Arrowheads point to two CCAP neurons not expressing the neuropeptide. (G) Summary of phenotypes.
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BIO012872F5: Abd-B represses the expression of CCAP and Bursα. (A,B) Expression of Dac (green) and Bursα (red) in Abd-BM1 ganglia of larvae 1. Higher magnification views of Bursα expression in abdominal segments (A′) and in segments A6–7 (B) are shown in white. The red bar indicates the thorax/abdomen separation. Note in B the presence of two neurons per hemisegment and the efferent axons (red arrowheads). (C) Expression of Bursα (green), CCAP (red) and Dac (blue) in elav-Gal4 UAS-Abd-B-RNAi UAS-dicer2 larvae 2. A higher magnification view of segment A7 (arrowhead) is shown on the right. (D) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-Abd-BM2. Bursα expression is absent in both INs and ENs. (E) CCAP expression in Df(3L)H99 elav-Gal4 UAS-Abd-BM2. (F) Expression of GFP (green) and CCAP (red) in CCAP-Gal4 UAS-GFP UAS-Abd-BM2 in larva 3. A higher magnification view of the boxed area is shown on the right. Arrowheads point to two CCAP neurons not expressing the neuropeptide. (G) Summary of phenotypes.

Mentions: To directly test whether Abd-B has a role in the specification of CCAP/Bursα in segments A5–7, we stained for Bursα in the strong hypomorph allele Abd-BM1. This allele removes both Abd-B isoforms (m and r) (Casanova et al., 1986). In Abd-BM1 first instar larvae we observed one EN per hemisegment in segments A5–7 and also two extra CCAP neurons in segments A8-9 (Fig. 5A-A′and G). These ENs in segments A5–7, which normally undergo terminal differentiation expressing CCAP and projecting efferent axons in pupae, possessed efferent axons in larva 1 (Fig. 5B). We obtained the same result with other strong Abd-B alleles (Abd-BDf(3R)C4, Abd-BD18, Abd-BD16 and Abd-BM5; supplementary material Fig. S4G-I and data not shown). CCAP-ENs seem to be very sensitive to the levels of Abd-B expression because in Abd-BM5/+ first instar larvae we observed CCAP-ENs in segments A5 (supplementary material Fig. S4J).Fig. 5.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Abd-B represses the expression of CCAP and Bursα. (A,B) Expression of Dac (green) and Bursα (red) in Abd-BM1 ganglia of larvae 1. Higher magnification views of Bursα expression in abdominal segments (A′) and in segments A6–7 (B) are shown in white. The red bar indicates the thorax/abdomen separation. Note in B the presence of two neurons per hemisegment and the efferent axons (red arrowheads). (C) Expression of Bursα (green), CCAP (red) and Dac (blue) in elav-Gal4 UAS-Abd-B-RNAi UAS-dicer2 larvae 2. A higher magnification view of segment A7 (arrowhead) is shown on the right. (D) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-Abd-BM2. Bursα expression is absent in both INs and ENs. (E) CCAP expression in Df(3L)H99 elav-Gal4 UAS-Abd-BM2. (F) Expression of GFP (green) and CCAP (red) in CCAP-Gal4 UAS-GFP UAS-Abd-BM2 in larva 3. A higher magnification view of the boxed area is shown on the right. Arrowheads point to two CCAP neurons not expressing the neuropeptide. (G) Summary of phenotypes.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F5: Abd-B represses the expression of CCAP and Bursα. (A,B) Expression of Dac (green) and Bursα (red) in Abd-BM1 ganglia of larvae 1. Higher magnification views of Bursα expression in abdominal segments (A′) and in segments A6–7 (B) are shown in white. The red bar indicates the thorax/abdomen separation. Note in B the presence of two neurons per hemisegment and the efferent axons (red arrowheads). (C) Expression of Bursα (green), CCAP (red) and Dac (blue) in elav-Gal4 UAS-Abd-B-RNAi UAS-dicer2 larvae 2. A higher magnification view of segment A7 (arrowhead) is shown on the right. (D) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-Abd-BM2. Bursα expression is absent in both INs and ENs. (E) CCAP expression in Df(3L)H99 elav-Gal4 UAS-Abd-BM2. (F) Expression of GFP (green) and CCAP (red) in CCAP-Gal4 UAS-GFP UAS-Abd-BM2 in larva 3. A higher magnification view of the boxed area is shown on the right. Arrowheads point to two CCAP neurons not expressing the neuropeptide. (G) Summary of phenotypes.
Mentions: To directly test whether Abd-B has a role in the specification of CCAP/Bursα in segments A5–7, we stained for Bursα in the strong hypomorph allele Abd-BM1. This allele removes both Abd-B isoforms (m and r) (Casanova et al., 1986). In Abd-BM1 first instar larvae we observed one EN per hemisegment in segments A5–7 and also two extra CCAP neurons in segments A8-9 (Fig. 5A-A′and G). These ENs in segments A5–7, which normally undergo terminal differentiation expressing CCAP and projecting efferent axons in pupae, possessed efferent axons in larva 1 (Fig. 5B). We obtained the same result with other strong Abd-B alleles (Abd-BDf(3R)C4, Abd-BD18, Abd-BD16 and Abd-BM5; supplementary material Fig. S4G-I and data not shown). CCAP-ENs seem to be very sensitive to the levels of Abd-B expression because in Abd-BM5/+ first instar larvae we observed CCAP-ENs in segments A5 (supplementary material Fig. S4J).Fig. 5.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus