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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus

Ubx is not required to maintain neuropeptide expression. (A,A′) Expression of Ubx (green), Bursα (red) and Hb (blue) in the T3 (A) and A3 (A′) segments of a wild-type ganglion. (B,B′) Expression of Ubx (green) and Bursα (red) in the T3 (B) and A3 (B′) segments of a Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2 ganglion.
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BIO012872F4: Ubx is not required to maintain neuropeptide expression. (A,A′) Expression of Ubx (green), Bursα (red) and Hb (blue) in the T3 (A) and A3 (A′) segments of a wild-type ganglion. (B,B′) Expression of Ubx (green) and Bursα (red) in the T3 (B) and A3 (B′) segments of a Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2 ganglion.

Mentions: A number of studies have shown that some genes involved in the initial specification of neuronal identity are also required for its maintenance in mature CNS (reviewed in Deneris and Hobert, 2014). In Drosophila this has been reported for a few cases (Eade et al., 2012; Estacio-Gomez et al., 2013; Hsiao et al., 2013; Jafari et al., 2012). We assessed whether maintenance of CCAP-EN identity requires permanent expression of Ubx. To this end, we stained for Ubx in third instar larvae and observed expression in the CCAPs of segments A1–4 but not in T3 (Fig. 4A). Next, we knocked down Ubx from first instar larva in individuals hemizygous for the BX-C (Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2) and did not observe any change in the pattern of Bursα expression in third instar larvae (Fig. 4B). Although we still observed low levels of Ubx expression in the CCAP neurons, this was probably due to perdurance of the protein made in the embryo, since Ubx expression is strongly inhibited in the imaginal discs of this line (D. Garaulet, personal communication). Taken together these findings support the conclusion that Ubx is not permanently required to maintain the fate of these neurons.Fig. 4.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Ubx is not required to maintain neuropeptide expression. (A,A′) Expression of Ubx (green), Bursα (red) and Hb (blue) in the T3 (A) and A3 (A′) segments of a wild-type ganglion. (B,B′) Expression of Ubx (green) and Bursα (red) in the T3 (B) and A3 (B′) segments of a Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2 ganglion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F4: Ubx is not required to maintain neuropeptide expression. (A,A′) Expression of Ubx (green), Bursα (red) and Hb (blue) in the T3 (A) and A3 (A′) segments of a wild-type ganglion. (B,B′) Expression of Ubx (green) and Bursα (red) in the T3 (B) and A3 (B′) segments of a Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2 ganglion.
Mentions: A number of studies have shown that some genes involved in the initial specification of neuronal identity are also required for its maintenance in mature CNS (reviewed in Deneris and Hobert, 2014). In Drosophila this has been reported for a few cases (Eade et al., 2012; Estacio-Gomez et al., 2013; Hsiao et al., 2013; Jafari et al., 2012). We assessed whether maintenance of CCAP-EN identity requires permanent expression of Ubx. To this end, we stained for Ubx in third instar larvae and observed expression in the CCAPs of segments A1–4 but not in T3 (Fig. 4A). Next, we knocked down Ubx from first instar larva in individuals hemizygous for the BX-C (Df(3R)109/+ elav-Gal4 UAS-dsUbx UAS-dicer2) and did not observe any change in the pattern of Bursα expression in third instar larvae (Fig. 4B). Although we still observed low levels of Ubx expression in the CCAP neurons, this was probably due to perdurance of the protein made in the embryo, since Ubx expression is strongly inhibited in the imaginal discs of this line (D. Garaulet, personal communication). Taken together these findings support the conclusion that Ubx is not permanently required to maintain the fate of these neurons.Fig. 4.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus