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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus

Effects of misexpressing Ubx or abd-A on the pattern of CCAP neurons. (A,A′) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-UAS-UbxIAI. (B-C′) Expression of Bursα (green), CCAP (red) and Dac (blue) in Df(3L)H99 (B,B′) and Df(3L)H99 Ubx6.28 (C,C′). (D,D′) Expression of Dac (green) and Bursα (red) in Df(3L)H99 Ubx6.28 abd-AM1. Closer views of segments are shown at the bottom of each figure. (E-F′) Bursα (green) and Lk (red) expression in elav-Gal4 UAS-abd-A (E,E′) and Df(3L)H99 elav-Gal4 UAS-abd-A (F,F′). Bursα expression is shown separately on the right of each figure. All samples are first instar larva. (G) Summary of phenotypes. White bars indicate boundaries between subesophagic, thoracic and abdominal segments (A-C′) or between thoracic and abdominal segments (E,F).
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BIO012872F3: Effects of misexpressing Ubx or abd-A on the pattern of CCAP neurons. (A,A′) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-UAS-UbxIAI. (B-C′) Expression of Bursα (green), CCAP (red) and Dac (blue) in Df(3L)H99 (B,B′) and Df(3L)H99 Ubx6.28 (C,C′). (D,D′) Expression of Dac (green) and Bursα (red) in Df(3L)H99 Ubx6.28 abd-AM1. Closer views of segments are shown at the bottom of each figure. (E-F′) Bursα (green) and Lk (red) expression in elav-Gal4 UAS-abd-A (E,E′) and Df(3L)H99 elav-Gal4 UAS-abd-A (F,F′). Bursα expression is shown separately on the right of each figure. All samples are first instar larva. (G) Summary of phenotypes. White bars indicate boundaries between subesophagic, thoracic and abdominal segments (A-C′) or between thoracic and abdominal segments (E,F).

Mentions: Next, we assessed the effect of misexpressing Ubx in post-mitotic cells. To that end we used elav-Gal4 (elav-Gal4 UAS-UAS-UbxIAI). Although it has been reported that elav-Gal4 is also expressed in neuroblasts and therefore behaves as a pan-neural driver in late stages of neurogenesis, in earlier stages, when CCAP neurons are generates, its expression is restricted to postmitotic cells (Berger et al., 2007), thus in these experiments can be consider a postmitotic driver. We observed one extra CCAP-EN per hemisegment in segments SE1-T2, while the fate of the CCAP-INs was unaffected (Fig. 3A-A′,G). This phenotype was also observed in programmed cell death-deficient larvae (Df(3L)H99) (Fig. 3B-B′). These findings suggest that, in normal development, the CCAP-ENs in segments SE1–T2 die by apoptosis but can be rescued by misexpression of Ubx. In accord with this conclusion, CCAP-ENs were present in all segments from SE1 to A4 in both Df(3L)H99 Ubx6.28 and Df(3L)H99 UbxMX6 abd-AM1 larvae (Fig. 3C-D′). Thus, preventing cell death not only rescues the CCAP-ENs of segments SE1–T2 but also those lost in segments T3–A1 in the Ubx mutant and in segments T3–A4 in the Ubx abd-A double mutant.Fig. 3.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ - Biol Open (2015)

Effects of misexpressing Ubx or abd-A on the pattern of CCAP neurons. (A,A′) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-UAS-UbxIAI. (B-C′) Expression of Bursα (green), CCAP (red) and Dac (blue) in Df(3L)H99 (B,B′) and Df(3L)H99 Ubx6.28 (C,C′). (D,D′) Expression of Dac (green) and Bursα (red) in Df(3L)H99 Ubx6.28 abd-AM1. Closer views of segments are shown at the bottom of each figure. (E-F′) Bursα (green) and Lk (red) expression in elav-Gal4 UAS-abd-A (E,E′) and Df(3L)H99 elav-Gal4 UAS-abd-A (F,F′). Bursα expression is shown separately on the right of each figure. All samples are first instar larva. (G) Summary of phenotypes. White bars indicate boundaries between subesophagic, thoracic and abdominal segments (A-C′) or between thoracic and abdominal segments (E,F).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F3: Effects of misexpressing Ubx or abd-A on the pattern of CCAP neurons. (A,A′) Expression of Dac (green) and Bursα (red) in elav-Gal4 UAS-UAS-UbxIAI. (B-C′) Expression of Bursα (green), CCAP (red) and Dac (blue) in Df(3L)H99 (B,B′) and Df(3L)H99 Ubx6.28 (C,C′). (D,D′) Expression of Dac (green) and Bursα (red) in Df(3L)H99 Ubx6.28 abd-AM1. Closer views of segments are shown at the bottom of each figure. (E-F′) Bursα (green) and Lk (red) expression in elav-Gal4 UAS-abd-A (E,E′) and Df(3L)H99 elav-Gal4 UAS-abd-A (F,F′). Bursα expression is shown separately on the right of each figure. All samples are first instar larva. (G) Summary of phenotypes. White bars indicate boundaries between subesophagic, thoracic and abdominal segments (A-C′) or between thoracic and abdominal segments (E,F).
Mentions: Next, we assessed the effect of misexpressing Ubx in post-mitotic cells. To that end we used elav-Gal4 (elav-Gal4 UAS-UAS-UbxIAI). Although it has been reported that elav-Gal4 is also expressed in neuroblasts and therefore behaves as a pan-neural driver in late stages of neurogenesis, in earlier stages, when CCAP neurons are generates, its expression is restricted to postmitotic cells (Berger et al., 2007), thus in these experiments can be consider a postmitotic driver. We observed one extra CCAP-EN per hemisegment in segments SE1-T2, while the fate of the CCAP-INs was unaffected (Fig. 3A-A′,G). This phenotype was also observed in programmed cell death-deficient larvae (Df(3L)H99) (Fig. 3B-B′). These findings suggest that, in normal development, the CCAP-ENs in segments SE1–T2 die by apoptosis but can be rescued by misexpression of Ubx. In accord with this conclusion, CCAP-ENs were present in all segments from SE1 to A4 in both Df(3L)H99 Ubx6.28 and Df(3L)H99 UbxMX6 abd-AM1 larvae (Fig. 3C-D′). Thus, preventing cell death not only rescues the CCAP-ENs of segments SE1–T2 but also those lost in segments T3–A1 in the Ubx mutant and in segments T3–A4 in the Ubx abd-A double mutant.Fig. 3.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biología Molecular-Severo Ochoa (CSIC-UAM), c./Nicolás Cabrera 1, Universidad Autónoma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus