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The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-GĆ³mez A, SĆ”nchez-Herrero E, DĆ­az-Benjumea FJ - Biol Open (2015)

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de BiologĆ­a Molecular-Severo Ochoa (CSIC-UAM), c./NicolĆ”s Cabrera 1, Universidad AutĆ³noma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus

Patternof CCAP neurons in Ubx and abd-A mutants. (A-Bā€²) Expression in the CCAP neurons of segment A3 of (A) Ubx (green), BursĪ± (red) and Hb (blue); (B,Bā€²) Ī²-Galactosidase (green), BursĪ± (red) and Dac (blue) expression in abd-A-lacZ. Here, the CCAP-IN is identified by stronger expression of Hb (Moris-Sanz et al., 2014). (C) Expression of Ubx (green) and Ems (red) in the NB3-5 cluster of stage 11; a drawing depicting the observed pattern is shown on the right. (D-Dā€²) Expression of Abd-A (green) and Ems (red) in the Ems-expressing NBs (3-3, 3-5 and 4-4) at stage 12. Abd-A expression is weak in all NBs. (E) Expression of Dac (green) and BursĪ± (red) in Ubx6.28. (Eā€²) Magnified view of the boxed area in E. (F) Expression of BursĪ± in abd-AM1. (G) Expression of Dac (green) and BursĪ± (red) in UbxMX6 abd-AM1. BursĪ± expression is shown separately in gray (Gā€²). A higher magnification view of segment T3 (arrowhead) is shown at the bottom. (H) Diagram of the pattern of expression of Ubx (red), Abd-A (green) and Abd-B (blue) along the anteroposterior axis of the Drosophila VNC. The segmental and parasegmental units are indicated. (I) Summary of phenotypes. White bars in (E-G) indicate the boundary between thoracic and abdominal segments. T, thorax; A, abdomen.
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BIO012872F2: Patternof CCAP neurons in Ubx and abd-A mutants. (A-Bā€²) Expression in the CCAP neurons of segment A3 of (A) Ubx (green), BursĪ± (red) and Hb (blue); (B,Bā€²) Ī²-Galactosidase (green), BursĪ± (red) and Dac (blue) expression in abd-A-lacZ. Here, the CCAP-IN is identified by stronger expression of Hb (Moris-Sanz et al., 2014). (C) Expression of Ubx (green) and Ems (red) in the NB3-5 cluster of stage 11; a drawing depicting the observed pattern is shown on the right. (D-Dā€²) Expression of Abd-A (green) and Ems (red) in the Ems-expressing NBs (3-3, 3-5 and 4-4) at stage 12. Abd-A expression is weak in all NBs. (E) Expression of Dac (green) and BursĪ± (red) in Ubx6.28. (Eā€²) Magnified view of the boxed area in E. (F) Expression of BursĪ± in abd-AM1. (G) Expression of Dac (green) and BursĪ± (red) in UbxMX6 abd-AM1. BursĪ± expression is shown separately in gray (Gā€²). A higher magnification view of segment T3 (arrowhead) is shown at the bottom. (H) Diagram of the pattern of expression of Ubx (red), Abd-A (green) and Abd-B (blue) along the anteroposterior axis of the Drosophila VNC. The segmental and parasegmental units are indicated. (I) Summary of phenotypes. White bars in (E-G) indicate the boundary between thoracic and abdominal segments. T, thorax; A, abdomen.

Mentions: In the CNS the expression of Ubx is weak in parasegment (PS)5, strong in PS6 and weakens progressively in more posterior segments (Beachy et al., 1985; White and Wilcox, 1985). This posterior decline of Ubx expression is caused by both Abd-A and the expression of non-coding RNAs harboured in the BX-C (iab-4 and iab-8) (Bender, 2008; Struhl and White, 1985; Thomsen et al., 2010). Abd-A expression is detected from PS7 to PS12 (Karch et al., 1990; Macias et al., 1990). In each PS, Abd-A expression is stronger in the more anterior cells, where it turns off Ubx, thus resulting in complementary PS patterns (Gebelein and Mann, 2007; Karch et al., 1990) (Fig.Ā 2H).Fig. 2.


The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B.

Moris-Sanz M, Estacio-GĆ³mez A, SĆ”nchez-Herrero E, DĆ­az-Benjumea FJ - Biol Open (2015)

Patternof CCAP neurons in Ubx and abd-A mutants. (A-Bā€²) Expression in the CCAP neurons of segment A3 of (A) Ubx (green), BursĪ± (red) and Hb (blue); (B,Bā€²) Ī²-Galactosidase (green), BursĪ± (red) and Dac (blue) expression in abd-A-lacZ. Here, the CCAP-IN is identified by stronger expression of Hb (Moris-Sanz et al., 2014). (C) Expression of Ubx (green) and Ems (red) in the NB3-5 cluster of stage 11; a drawing depicting the observed pattern is shown on the right. (D-Dā€²) Expression of Abd-A (green) and Ems (red) in the Ems-expressing NBs (3-3, 3-5 and 4-4) at stage 12. Abd-A expression is weak in all NBs. (E) Expression of Dac (green) and BursĪ± (red) in Ubx6.28. (Eā€²) Magnified view of the boxed area in E. (F) Expression of BursĪ± in abd-AM1. (G) Expression of Dac (green) and BursĪ± (red) in UbxMX6 abd-AM1. BursĪ± expression is shown separately in gray (Gā€²). A higher magnification view of segment T3 (arrowhead) is shown at the bottom. (H) Diagram of the pattern of expression of Ubx (red), Abd-A (green) and Abd-B (blue) along the anteroposterior axis of the Drosophila VNC. The segmental and parasegmental units are indicated. (I) Summary of phenotypes. White bars in (E-G) indicate the boundary between thoracic and abdominal segments. T, thorax; A, abdomen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582124&req=5

BIO012872F2: Patternof CCAP neurons in Ubx and abd-A mutants. (A-Bā€²) Expression in the CCAP neurons of segment A3 of (A) Ubx (green), BursĪ± (red) and Hb (blue); (B,Bā€²) Ī²-Galactosidase (green), BursĪ± (red) and Dac (blue) expression in abd-A-lacZ. Here, the CCAP-IN is identified by stronger expression of Hb (Moris-Sanz et al., 2014). (C) Expression of Ubx (green) and Ems (red) in the NB3-5 cluster of stage 11; a drawing depicting the observed pattern is shown on the right. (D-Dā€²) Expression of Abd-A (green) and Ems (red) in the Ems-expressing NBs (3-3, 3-5 and 4-4) at stage 12. Abd-A expression is weak in all NBs. (E) Expression of Dac (green) and BursĪ± (red) in Ubx6.28. (Eā€²) Magnified view of the boxed area in E. (F) Expression of BursĪ± in abd-AM1. (G) Expression of Dac (green) and BursĪ± (red) in UbxMX6 abd-AM1. BursĪ± expression is shown separately in gray (Gā€²). A higher magnification view of segment T3 (arrowhead) is shown at the bottom. (H) Diagram of the pattern of expression of Ubx (red), Abd-A (green) and Abd-B (blue) along the anteroposterior axis of the Drosophila VNC. The segmental and parasegmental units are indicated. (I) Summary of phenotypes. White bars in (E-G) indicate the boundary between thoracic and abdominal segments. T, thorax; A, abdomen.
Mentions: In the CNS the expression of Ubx is weak in parasegment (PS)5, strong in PS6 and weakens progressively in more posterior segments (Beachy et al., 1985; White and Wilcox, 1985). This posterior decline of Ubx expression is caused by both Abd-A and the expression of non-coding RNAs harboured in the BX-C (iab-4 and iab-8) (Bender, 2008; Struhl and White, 1985; Thomsen et al., 2010). Abd-A expression is detected from PS7 to PS12 (Karch et al., 1990; Macias et al., 1990). In each PS, Abd-A expression is stronger in the more anterior cells, where it turns off Ubx, thus resulting in complementary PS patterns (Gebelein and Mann, 2007; Karch et al., 1990) (Fig.Ā 2H).Fig. 2.

Bottom Line: Our results indicate that Ultrabithorax and Abdominal-A are not necessary for specification of the CCAP-interneurons, but are absolutely required to prevent the death by apoptosis of the CCAP-efferent neurons.Furthermore, Abdominal-B controls by repression the temporal onset of neuropeptide expression in a subset of CCAP-efferent neurons, and a peak of ecdysone hormone at the end of larval life counteracts this repression.Thus, Bithorax complex genes control the developmental appearance of these neuropeptides both temporally and spatially.

View Article: PubMed Central - PubMed

Affiliation: Centro de BiologĆ­a Molecular-Severo Ochoa (CSIC-UAM), c./NicolĆ”s Cabrera 1, Universidad AutĆ³noma, Madrid 28049, Spain.

No MeSH data available.


Related in: MedlinePlus