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Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus

Mitochondrion-rich cells at polyp-stolon junctions in surgically manipulated colonies of Podocoryna carnea. Half of each of the 16 colonies was removed except for three polyps. The other (control) half was undisturbed. After 1–2 weeks, levels of reactive oxygen species were assayed by H2DCFDA fluorescence (mean±s.e.m.) for the three manipulated polyps (unfilled bars) and three comparable control polyps (filled bars). Numbers of mitochondrion-rich cells were also counted for manipulated (lower number) and control (upper number) polyps. To ensure rapid measurement of all colonies, experiments were carried out in groups of 4 colonies.
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BIO012187F8: Mitochondrion-rich cells at polyp-stolon junctions in surgically manipulated colonies of Podocoryna carnea. Half of each of the 16 colonies was removed except for three polyps. The other (control) half was undisturbed. After 1–2 weeks, levels of reactive oxygen species were assayed by H2DCFDA fluorescence (mean±s.e.m.) for the three manipulated polyps (unfilled bars) and three comparable control polyps (filled bars). Numbers of mitochondrion-rich cells were also counted for manipulated (lower number) and control (upper number) polyps. To ensure rapid measurement of all colonies, experiments were carried out in groups of 4 colonies.

Mentions: To further examine the functional biology of mitochondrion-rich cells, an experiment using microsurgery was carried out. Control polyps exhibited more mitochondrion-rich cells (Fig. 8), but this difference was not significant (N=16; t=1.76; P=0.1). Control polyps also exhibited greater mean relative luminance of mitochondrion-rich cells (Fig. 8), and this difference was highly significant (N=16; t=4.87; P=0.0002). Eliminating stolons thus diminished the ROS of mitochondrion-rich cells.Fig. 8.


Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Mitochondrion-rich cells at polyp-stolon junctions in surgically manipulated colonies of Podocoryna carnea. Half of each of the 16 colonies was removed except for three polyps. The other (control) half was undisturbed. After 1–2 weeks, levels of reactive oxygen species were assayed by H2DCFDA fluorescence (mean±s.e.m.) for the three manipulated polyps (unfilled bars) and three comparable control polyps (filled bars). Numbers of mitochondrion-rich cells were also counted for manipulated (lower number) and control (upper number) polyps. To ensure rapid measurement of all colonies, experiments were carried out in groups of 4 colonies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582120&req=5

BIO012187F8: Mitochondrion-rich cells at polyp-stolon junctions in surgically manipulated colonies of Podocoryna carnea. Half of each of the 16 colonies was removed except for three polyps. The other (control) half was undisturbed. After 1–2 weeks, levels of reactive oxygen species were assayed by H2DCFDA fluorescence (mean±s.e.m.) for the three manipulated polyps (unfilled bars) and three comparable control polyps (filled bars). Numbers of mitochondrion-rich cells were also counted for manipulated (lower number) and control (upper number) polyps. To ensure rapid measurement of all colonies, experiments were carried out in groups of 4 colonies.
Mentions: To further examine the functional biology of mitochondrion-rich cells, an experiment using microsurgery was carried out. Control polyps exhibited more mitochondrion-rich cells (Fig. 8), but this difference was not significant (N=16; t=1.76; P=0.1). Control polyps also exhibited greater mean relative luminance of mitochondrion-rich cells (Fig. 8), and this difference was highly significant (N=16; t=4.87; P=0.0002). Eliminating stolons thus diminished the ROS of mitochondrion-rich cells.Fig. 8.

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus