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Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus

Levels of reactive oxygen species in mitochondrion-rich cells of colonies of Podocoryna carnea as measured by H2DCFDA fluorescence. Mean±s.e.m. relative luminance (foreground minus background fluorescence, gray scale 0–4095) of mitochondrion-rich cells for three polyp-stolon junctions per colony are shown. Colonies treated with 2,4-dinitrophenol (open bars) produce overall significantly less reactive oxygen species than control colonies (filled bars). To ensure rapid measurement of all colonies, experiments were carried out in groups of 3–4 colonies. Colonies are paired in the order they were measured.
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BIO012187F7: Levels of reactive oxygen species in mitochondrion-rich cells of colonies of Podocoryna carnea as measured by H2DCFDA fluorescence. Mean±s.e.m. relative luminance (foreground minus background fluorescence, gray scale 0–4095) of mitochondrion-rich cells for three polyp-stolon junctions per colony are shown. Colonies treated with 2,4-dinitrophenol (open bars) produce overall significantly less reactive oxygen species than control colonies (filled bars). To ensure rapid measurement of all colonies, experiments were carried out in groups of 3–4 colonies. Colonies are paired in the order they were measured.

Mentions: Previous investigations of uncouplers (Blackstone, 1998b) focused on the redox state of mitochondrion-rich cells by examining the fluorescence of NAD(P)H. Here, complementary experiments were carried out examining ROS. As measured by relative luminance, control mitochondrion-rich cells overall exhibited greater fluorescence than those treated with DNP (grand mean±s.e.m. 1369.2±9.8 vs 1232.8±8.4). Using colonies within treatment as the error variance in an analysis of variance, relative luminance showed a significant difference between treatments (Fig. 7; F=7.2; d.f.=1,32; P=0.01). Examination of the coefficients of the variance components suggested that the F statistic was slightly conservative. The foreground area of the control mitochondrion-rich cells was also slightly larger than that of the treated (grand mean±s.e.m. 9.1±0.05 and 8.7±0.05 µm2) but not significantly so (F=2.6; d.f.=1,32; P=0.12). Treated colonies exhibited significantly greater background fluorescence than controls (F=5.5; d.f.=1,32; P=0.03). No difference in the number of mitochondrion-rich cells was found (F=0.8; d.f.=1,32; P=0.78).Fig. 7.


Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Levels of reactive oxygen species in mitochondrion-rich cells of colonies of Podocoryna carnea as measured by H2DCFDA fluorescence. Mean±s.e.m. relative luminance (foreground minus background fluorescence, gray scale 0–4095) of mitochondrion-rich cells for three polyp-stolon junctions per colony are shown. Colonies treated with 2,4-dinitrophenol (open bars) produce overall significantly less reactive oxygen species than control colonies (filled bars). To ensure rapid measurement of all colonies, experiments were carried out in groups of 3–4 colonies. Colonies are paired in the order they were measured.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582120&req=5

BIO012187F7: Levels of reactive oxygen species in mitochondrion-rich cells of colonies of Podocoryna carnea as measured by H2DCFDA fluorescence. Mean±s.e.m. relative luminance (foreground minus background fluorescence, gray scale 0–4095) of mitochondrion-rich cells for three polyp-stolon junctions per colony are shown. Colonies treated with 2,4-dinitrophenol (open bars) produce overall significantly less reactive oxygen species than control colonies (filled bars). To ensure rapid measurement of all colonies, experiments were carried out in groups of 3–4 colonies. Colonies are paired in the order they were measured.
Mentions: Previous investigations of uncouplers (Blackstone, 1998b) focused on the redox state of mitochondrion-rich cells by examining the fluorescence of NAD(P)H. Here, complementary experiments were carried out examining ROS. As measured by relative luminance, control mitochondrion-rich cells overall exhibited greater fluorescence than those treated with DNP (grand mean±s.e.m. 1369.2±9.8 vs 1232.8±8.4). Using colonies within treatment as the error variance in an analysis of variance, relative luminance showed a significant difference between treatments (Fig. 7; F=7.2; d.f.=1,32; P=0.01). Examination of the coefficients of the variance components suggested that the F statistic was slightly conservative. The foreground area of the control mitochondrion-rich cells was also slightly larger than that of the treated (grand mean±s.e.m. 9.1±0.05 and 8.7±0.05 µm2) but not significantly so (F=2.6; d.f.=1,32; P=0.12). Treated colonies exhibited significantly greater background fluorescence than controls (F=5.5; d.f.=1,32; P=0.03). No difference in the number of mitochondrion-rich cells was found (F=0.8; d.f.=1,32; P=0.78).Fig. 7.

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus