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Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus

Confocal sections of a polyp-stolon junction from a living colony of Podocoryna carnea treated with H2DCFDA. A negative control (A-F) and a treated colony (G-L) are shown. Starting with the base (A and G), the panels (B-F and H-L) depict every 10th optical section of a polyp-stolon junction. Each section is 0.47 µm thick. MRCs, mitochondrion-rich cells. Scale bar: 50 µm.
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BIO012187F5: Confocal sections of a polyp-stolon junction from a living colony of Podocoryna carnea treated with H2DCFDA. A negative control (A-F) and a treated colony (G-L) are shown. Starting with the base (A and G), the panels (B-F and H-L) depict every 10th optical section of a polyp-stolon junction. Each section is 0.47 µm thick. MRCs, mitochondrion-rich cells. Scale bar: 50 µm.

Mentions: Fluorescence of rhodamine 123, a mitochondrial probe (Johnson et al., 1980), also co-localized with NAD(P)H emissions (Fig. 3). On the other hand, Hoechst 33342, a nuclear probe (Dunn et al., 2012), did not co-localize with H2DCFDA emissions (Fig. 4). H2DCFDA-derived fluorescence was also examined using confocal microscopy, which shows fluorescence primarily localized to columnar cells in the upper part of the poly-stolon junction (Fig. 5). Three-dimensional reconstruction of the image stack shows two crests of mitochondrion-rich cells on both sides of the polyp-stolon junction (data not shown).Fig. 3.


Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Confocal sections of a polyp-stolon junction from a living colony of Podocoryna carnea treated with H2DCFDA. A negative control (A-F) and a treated colony (G-L) are shown. Starting with the base (A and G), the panels (B-F and H-L) depict every 10th optical section of a polyp-stolon junction. Each section is 0.47 µm thick. MRCs, mitochondrion-rich cells. Scale bar: 50 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582120&req=5

BIO012187F5: Confocal sections of a polyp-stolon junction from a living colony of Podocoryna carnea treated with H2DCFDA. A negative control (A-F) and a treated colony (G-L) are shown. Starting with the base (A and G), the panels (B-F and H-L) depict every 10th optical section of a polyp-stolon junction. Each section is 0.47 µm thick. MRCs, mitochondrion-rich cells. Scale bar: 50 µm.
Mentions: Fluorescence of rhodamine 123, a mitochondrial probe (Johnson et al., 1980), also co-localized with NAD(P)H emissions (Fig. 3). On the other hand, Hoechst 33342, a nuclear probe (Dunn et al., 2012), did not co-localize with H2DCFDA emissions (Fig. 4). H2DCFDA-derived fluorescence was also examined using confocal microscopy, which shows fluorescence primarily localized to columnar cells in the upper part of the poly-stolon junction (Fig. 5). Three-dimensional reconstruction of the image stack shows two crests of mitochondrion-rich cells on both sides of the polyp-stolon junction (data not shown).Fig. 3.

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus