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Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus

Micrographs of fluorescent emissions from a polyp-stolon junction in a living colony of Podocoryna carnea simultaneously treated with Hoechst 33342 and H2DCFDA. For this colony, the same polyp-stolon junction is shown at two different magnifications with ultraviolet excitation, blue emission, which visualizes nuclei (A,D) and blue excitation, green emission, which visualizes ROS (B,E). Merged, pseudocolored images are also shown (C,F). With a region of interest confined to the polyp-stolon junction, co-localization (Pearson's correlation, rp) of the two probes is −0.07 (C) and 0.04 (F), which suggests little or no co-localization. MRCs, mitochondrion-rich cells (exclusive of their nuclei). Scale bars: A-C, 20 µm; D-F, 10 µm.
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BIO012187F4: Micrographs of fluorescent emissions from a polyp-stolon junction in a living colony of Podocoryna carnea simultaneously treated with Hoechst 33342 and H2DCFDA. For this colony, the same polyp-stolon junction is shown at two different magnifications with ultraviolet excitation, blue emission, which visualizes nuclei (A,D) and blue excitation, green emission, which visualizes ROS (B,E). Merged, pseudocolored images are also shown (C,F). With a region of interest confined to the polyp-stolon junction, co-localization (Pearson's correlation, rp) of the two probes is −0.07 (C) and 0.04 (F), which suggests little or no co-localization. MRCs, mitochondrion-rich cells (exclusive of their nuclei). Scale bars: A-C, 20 µm; D-F, 10 µm.

Mentions: Fluorescence of rhodamine 123, a mitochondrial probe (Johnson et al., 1980), also co-localized with NAD(P)H emissions (Fig. 3). On the other hand, Hoechst 33342, a nuclear probe (Dunn et al., 2012), did not co-localize with H2DCFDA emissions (Fig. 4). H2DCFDA-derived fluorescence was also examined using confocal microscopy, which shows fluorescence primarily localized to columnar cells in the upper part of the poly-stolon junction (Fig. 5). Three-dimensional reconstruction of the image stack shows two crests of mitochondrion-rich cells on both sides of the polyp-stolon junction (data not shown).Fig. 3.


Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Micrographs of fluorescent emissions from a polyp-stolon junction in a living colony of Podocoryna carnea simultaneously treated with Hoechst 33342 and H2DCFDA. For this colony, the same polyp-stolon junction is shown at two different magnifications with ultraviolet excitation, blue emission, which visualizes nuclei (A,D) and blue excitation, green emission, which visualizes ROS (B,E). Merged, pseudocolored images are also shown (C,F). With a region of interest confined to the polyp-stolon junction, co-localization (Pearson's correlation, rp) of the two probes is −0.07 (C) and 0.04 (F), which suggests little or no co-localization. MRCs, mitochondrion-rich cells (exclusive of their nuclei). Scale bars: A-C, 20 µm; D-F, 10 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582120&req=5

BIO012187F4: Micrographs of fluorescent emissions from a polyp-stolon junction in a living colony of Podocoryna carnea simultaneously treated with Hoechst 33342 and H2DCFDA. For this colony, the same polyp-stolon junction is shown at two different magnifications with ultraviolet excitation, blue emission, which visualizes nuclei (A,D) and blue excitation, green emission, which visualizes ROS (B,E). Merged, pseudocolored images are also shown (C,F). With a region of interest confined to the polyp-stolon junction, co-localization (Pearson's correlation, rp) of the two probes is −0.07 (C) and 0.04 (F), which suggests little or no co-localization. MRCs, mitochondrion-rich cells (exclusive of their nuclei). Scale bars: A-C, 20 µm; D-F, 10 µm.
Mentions: Fluorescence of rhodamine 123, a mitochondrial probe (Johnson et al., 1980), also co-localized with NAD(P)H emissions (Fig. 3). On the other hand, Hoechst 33342, a nuclear probe (Dunn et al., 2012), did not co-localize with H2DCFDA emissions (Fig. 4). H2DCFDA-derived fluorescence was also examined using confocal microscopy, which shows fluorescence primarily localized to columnar cells in the upper part of the poly-stolon junction (Fig. 5). Three-dimensional reconstruction of the image stack shows two crests of mitochondrion-rich cells on both sides of the polyp-stolon junction (data not shown).Fig. 3.

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus