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Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus

Micrographs of fluorescent emissions from polyp-stolon junctions in living colonies of Podocoryna carnea treated with H2DCFDA. A polyp-stolon junction of a colony treated with H2DCFDA is shown with ultraviolet excitation, blue emission (A) and blue excitation, green emission (C). A polyp-stolon junction of a colony that was treated with an equivalent amount of DMSO (negative control), is shown with ultraviolet excitation, blue emission (B) and blue excitation, green emission (D). Identical camera settings were used for A and B, and for C and D. MRCs, mitochondrion-rich cells. Scale bar: 10 µm.
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BIO012187F2: Micrographs of fluorescent emissions from polyp-stolon junctions in living colonies of Podocoryna carnea treated with H2DCFDA. A polyp-stolon junction of a colony treated with H2DCFDA is shown with ultraviolet excitation, blue emission (A) and blue excitation, green emission (C). A polyp-stolon junction of a colony that was treated with an equivalent amount of DMSO (negative control), is shown with ultraviolet excitation, blue emission (B) and blue excitation, green emission (D). Identical camera settings were used for A and B, and for C and D. MRCs, mitochondrion-rich cells. Scale bar: 10 µm.

Mentions: Mitochondrion-rich cells are expected to emit large quantities of ROS (Chance et al., 1979). Hence, H2DCFDA should be oxidized by these cells in great quantities (Blackstone, 2001; Blackstone et al., 2004a,b). These results (Fig. 2) confirm earlier work (Blackstone, 2001, 2003; Blackstone et al., 2004a,b). With excitement in the ultraviolet and emission in the blue, the chitinous perisarc on the outside of the colony strongly fluoresced. The putative mitochondrion-rich cells also fluoresced. The NAD(P)H and chitin emissions were impossible to distinguish. In colonies that were incubated in H2DCFDA, however, the intense emission from DCF required setting the camera to much lower sensitivity. Native fluorescence, whether of FAD or chitin, was virtually undetectable at these settings (negative control, Fig. 2D). The mitochondrion-rich cells nevertheless strongly fluoresced (Fig. 2C). The negative controls showed that native fluorescence, whether of FAD or chitin, did not contribute at all to this fluorescence (compare Fig. 2C to D).Fig. 2.


Structure and signaling at hydroid polyp-stolon junctions, revisited.

Harmata KL, Somova EL, Parrin AP, Bross LS, Glockling SL, Blackstone NW - Biol Open (2015)

Micrographs of fluorescent emissions from polyp-stolon junctions in living colonies of Podocoryna carnea treated with H2DCFDA. A polyp-stolon junction of a colony treated with H2DCFDA is shown with ultraviolet excitation, blue emission (A) and blue excitation, green emission (C). A polyp-stolon junction of a colony that was treated with an equivalent amount of DMSO (negative control), is shown with ultraviolet excitation, blue emission (B) and blue excitation, green emission (D). Identical camera settings were used for A and B, and for C and D. MRCs, mitochondrion-rich cells. Scale bar: 10 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582120&req=5

BIO012187F2: Micrographs of fluorescent emissions from polyp-stolon junctions in living colonies of Podocoryna carnea treated with H2DCFDA. A polyp-stolon junction of a colony treated with H2DCFDA is shown with ultraviolet excitation, blue emission (A) and blue excitation, green emission (C). A polyp-stolon junction of a colony that was treated with an equivalent amount of DMSO (negative control), is shown with ultraviolet excitation, blue emission (B) and blue excitation, green emission (D). Identical camera settings were used for A and B, and for C and D. MRCs, mitochondrion-rich cells. Scale bar: 10 µm.
Mentions: Mitochondrion-rich cells are expected to emit large quantities of ROS (Chance et al., 1979). Hence, H2DCFDA should be oxidized by these cells in great quantities (Blackstone, 2001; Blackstone et al., 2004a,b). These results (Fig. 2) confirm earlier work (Blackstone, 2001, 2003; Blackstone et al., 2004a,b). With excitement in the ultraviolet and emission in the blue, the chitinous perisarc on the outside of the colony strongly fluoresced. The putative mitochondrion-rich cells also fluoresced. The NAD(P)H and chitin emissions were impossible to distinguish. In colonies that were incubated in H2DCFDA, however, the intense emission from DCF required setting the camera to much lower sensitivity. Native fluorescence, whether of FAD or chitin, was virtually undetectable at these settings (negative control, Fig. 2D). The mitochondrion-rich cells nevertheless strongly fluoresced (Fig. 2C). The negative controls showed that native fluorescence, whether of FAD or chitin, did not contribute at all to this fluorescence (compare Fig. 2C to D).Fig. 2.

Bottom Line: Transmission electron microscopy identified mitochondrion-rich cells adjacent to a thick layer of mesoglea at polyp-stolon junctions.The myonemes of these myoepithelial cells extend from the thickened mesoglea to the rigid perisarc on the outside of the colony.The perisarc thus anchors the myoepithelial cells and allows them to pull against the mesoglea and open the lumen of the polyp-stolon junction, while relaxation of these cells closes the lumen.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA.

No MeSH data available.


Related in: MedlinePlus