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Fluorescent proteins function as a prey attractant: experimental evidence from the hydromedusa Olindias formosus and other marine organisms.

Haddock SH, Dunn CW - Biol Open (2015)

Bottom Line: The fish did not respond significantly when treatments did not include fluorescent structures or took place under yellow or white lights, which did not generate fluorescence visible above the ambient light.In situ observations also provided evidence for fluorescent lures as supernormal stimuli in several other marine animals, including the siphonophore Rhizophysa eysenhardti.Our results support the idea that fluorescent structures can serve as prey attractants, thus providing a potential function for GFPs and other fluorescent proteins in a diverse range of organisms.

View Article: PubMed Central - PubMed

Affiliation: Monterey Bay Aquarium Research Institute (MBARI), 7700 Sandholdt Rd, Moss Landing, CA 95039-9644, USA haddock@mbari.org.

No MeSH data available.


Related in: MedlinePlus

Frame grabs from video of the green laser deployment underwater. (A-D) Great Barrier Reef, showing wrasses pursuing the laser across the bottom and biting at it. (E-H) Aquarium footage of a goatfish responding to the appearance of the laser. Interval from E-F is 330 ms, and images G and H are each at 100 ms intervals. In image G, the barbels, laden with taste sensors, are extended to investigate the dot.
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BIO012138F5: Frame grabs from video of the green laser deployment underwater. (A-D) Great Barrier Reef, showing wrasses pursuing the laser across the bottom and biting at it. (E-H) Aquarium footage of a goatfish responding to the appearance of the laser. Interval from E-F is 330 ms, and images G and H are each at 100 ms intervals. In image G, the barbels, laden with taste sensors, are extended to investigate the dot.

Mentions: To observe the natural responses of fishes to illuminated dots, similar to the view of fluorescent tentacle tips, we constructed an underwater housing for a green laser pointer (Fig. 4), and tested it in several locations as well as in aquaria. During the day, use of the green laser underwater elicited aggressive responses from many fish living on and around coral reefs, especially in sandy patches (Fig. 5; supplementary material Movie 1). Fish made repeated strikes at the point where the laser shone on the substrate. At the Great Barrier Reef, Australia these responses were most pronounced among the benthic species, including blennies (Enchelyurus spp.), Chromis, lizardfish (Synodus jaculum), gleaners such as wrasses (e.g. the black-spot wrasse, blue-headed wrasse), and goatfish (Mullidae, e.g. Mulloidichthys sp,, Parupeneus sp.). Blennies showed territorial behavior and aggressively chased the spot only for a few centimeters, but wrasses and goatfish pursued the spot persistently for many minutes and across many meters. In Western Papua, many kinds of fish, including young damselfish (Pomacentrus sp.), triggerfish (Balistapus sp.), wrasses and snappers (Lutjanus biguttatus) showed interest and aggression at the dot, including following it across the bottom and biting at it. At night, when the beam was relatively much brighter in the darkness, fish avoided the green beam of light, often swimming rapidly away or even leaping out of the water.Fig. 4.


Fluorescent proteins function as a prey attractant: experimental evidence from the hydromedusa Olindias formosus and other marine organisms.

Haddock SH, Dunn CW - Biol Open (2015)

Frame grabs from video of the green laser deployment underwater. (A-D) Great Barrier Reef, showing wrasses pursuing the laser across the bottom and biting at it. (E-H) Aquarium footage of a goatfish responding to the appearance of the laser. Interval from E-F is 330 ms, and images G and H are each at 100 ms intervals. In image G, the barbels, laden with taste sensors, are extended to investigate the dot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582119&req=5

BIO012138F5: Frame grabs from video of the green laser deployment underwater. (A-D) Great Barrier Reef, showing wrasses pursuing the laser across the bottom and biting at it. (E-H) Aquarium footage of a goatfish responding to the appearance of the laser. Interval from E-F is 330 ms, and images G and H are each at 100 ms intervals. In image G, the barbels, laden with taste sensors, are extended to investigate the dot.
Mentions: To observe the natural responses of fishes to illuminated dots, similar to the view of fluorescent tentacle tips, we constructed an underwater housing for a green laser pointer (Fig. 4), and tested it in several locations as well as in aquaria. During the day, use of the green laser underwater elicited aggressive responses from many fish living on and around coral reefs, especially in sandy patches (Fig. 5; supplementary material Movie 1). Fish made repeated strikes at the point where the laser shone on the substrate. At the Great Barrier Reef, Australia these responses were most pronounced among the benthic species, including blennies (Enchelyurus spp.), Chromis, lizardfish (Synodus jaculum), gleaners such as wrasses (e.g. the black-spot wrasse, blue-headed wrasse), and goatfish (Mullidae, e.g. Mulloidichthys sp,, Parupeneus sp.). Blennies showed territorial behavior and aggressively chased the spot only for a few centimeters, but wrasses and goatfish pursued the spot persistently for many minutes and across many meters. In Western Papua, many kinds of fish, including young damselfish (Pomacentrus sp.), triggerfish (Balistapus sp.), wrasses and snappers (Lutjanus biguttatus) showed interest and aggression at the dot, including following it across the bottom and biting at it. At night, when the beam was relatively much brighter in the darkness, fish avoided the green beam of light, often swimming rapidly away or even leaping out of the water.Fig. 4.

Bottom Line: The fish did not respond significantly when treatments did not include fluorescent structures or took place under yellow or white lights, which did not generate fluorescence visible above the ambient light.In situ observations also provided evidence for fluorescent lures as supernormal stimuli in several other marine animals, including the siphonophore Rhizophysa eysenhardti.Our results support the idea that fluorescent structures can serve as prey attractants, thus providing a potential function for GFPs and other fluorescent proteins in a diverse range of organisms.

View Article: PubMed Central - PubMed

Affiliation: Monterey Bay Aquarium Research Institute (MBARI), 7700 Sandholdt Rd, Moss Landing, CA 95039-9644, USA haddock@mbari.org.

No MeSH data available.


Related in: MedlinePlus