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Flagellar membranes are rich in raft-forming phospholipids.

Serricchio M, Schmid AW, Steinmann ME, Sigel E, Rauch M, Julkowska D, Bonnefoy S, Fort C, Bastin P, Bütikofer P - Biol Open (2015)

Bottom Line: Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells.Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella.Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry & Molecular Medicine, University of Bern, Bern 3012, Switzerland peter.buetikofer@ibmm.unibe.ch mauro.serricchio@utoronto.ca.

No MeSH data available.


Phospholipid composition of T. brucei parasites and flagella. Left panel: The phospholipid composition of parasites was determined by two-dimensional thin-layer chromatography and lipid phosphorus quantification. The data are mean values from four separate determinations. Right panel: The phospholipid composition of flagella was calculated from the data shown in the left panel using the mean relative changes observed in two LC-MS analyses between flagella and whole cells.
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BIO011957F7: Phospholipid composition of T. brucei parasites and flagella. Left panel: The phospholipid composition of parasites was determined by two-dimensional thin-layer chromatography and lipid phosphorus quantification. The data are mean values from four separate determinations. Right panel: The phospholipid composition of flagella was calculated from the data shown in the left panel using the mean relative changes observed in two LC-MS analyses between flagella and whole cells.

Mentions: The limited amounts of flagella available did not allow quantitative phospholipid analysis using lipid phosphorus determination. Nevertheless, based on our mass spectrometry data we were able to deduce the phospholipid composition of flagella membranes. For this we first determined the phospholipid composition of whole cells using two-dimensional thin layer chromatography followed by lipid phosphorus quantification (Fig. 7, left panel) and then calculated the phospholipid composition of flagella (Fig. 7, right panel) using the relative changes in individual phospholipid classes between whole cells and flagella determined by LC-MS analysis. The result shows that in contrast to whole cells, where PC is the most abundant phospholipid class, PE is the most abundant phospholipid class in flagella (increase from approximately 20% in whole cells to approximately 40% of total phospholipid in flagella). Accordingly, decreased relative amounts in flagella versus whole cells were observed for PC (37% vs 65%) and PI (2% vs 5%). In contrast, the relative amounts of PS, SM and IPC in flagella were increased compared to whole cells (3% vs 2%, 11% vs 5% and 6% vs 2%, respectively). Together, the relative amounts of the raft-typical sphingophospholipid classes, SM and IPC, were increased in flagella (17%) compared to whole cells (7%) (Fig. 7). These calculated numbers are in good agreement with the values determined by LC-MS/MS (Fig. 6E), where sphingophospholipid signal intensities accounted for 24% of total intensities in flagella and 12% in whole cells.Fig. 7.


Flagellar membranes are rich in raft-forming phospholipids.

Serricchio M, Schmid AW, Steinmann ME, Sigel E, Rauch M, Julkowska D, Bonnefoy S, Fort C, Bastin P, Bütikofer P - Biol Open (2015)

Phospholipid composition of T. brucei parasites and flagella. Left panel: The phospholipid composition of parasites was determined by two-dimensional thin-layer chromatography and lipid phosphorus quantification. The data are mean values from four separate determinations. Right panel: The phospholipid composition of flagella was calculated from the data shown in the left panel using the mean relative changes observed in two LC-MS analyses between flagella and whole cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582118&req=5

BIO011957F7: Phospholipid composition of T. brucei parasites and flagella. Left panel: The phospholipid composition of parasites was determined by two-dimensional thin-layer chromatography and lipid phosphorus quantification. The data are mean values from four separate determinations. Right panel: The phospholipid composition of flagella was calculated from the data shown in the left panel using the mean relative changes observed in two LC-MS analyses between flagella and whole cells.
Mentions: The limited amounts of flagella available did not allow quantitative phospholipid analysis using lipid phosphorus determination. Nevertheless, based on our mass spectrometry data we were able to deduce the phospholipid composition of flagella membranes. For this we first determined the phospholipid composition of whole cells using two-dimensional thin layer chromatography followed by lipid phosphorus quantification (Fig. 7, left panel) and then calculated the phospholipid composition of flagella (Fig. 7, right panel) using the relative changes in individual phospholipid classes between whole cells and flagella determined by LC-MS analysis. The result shows that in contrast to whole cells, where PC is the most abundant phospholipid class, PE is the most abundant phospholipid class in flagella (increase from approximately 20% in whole cells to approximately 40% of total phospholipid in flagella). Accordingly, decreased relative amounts in flagella versus whole cells were observed for PC (37% vs 65%) and PI (2% vs 5%). In contrast, the relative amounts of PS, SM and IPC in flagella were increased compared to whole cells (3% vs 2%, 11% vs 5% and 6% vs 2%, respectively). Together, the relative amounts of the raft-typical sphingophospholipid classes, SM and IPC, were increased in flagella (17%) compared to whole cells (7%) (Fig. 7). These calculated numbers are in good agreement with the values determined by LC-MS/MS (Fig. 6E), where sphingophospholipid signal intensities accounted for 24% of total intensities in flagella and 12% in whole cells.Fig. 7.

Bottom Line: Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells.Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella.Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry & Molecular Medicine, University of Bern, Bern 3012, Switzerland peter.buetikofer@ibmm.unibe.ch mauro.serricchio@utoronto.ca.

No MeSH data available.