Limits...
Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis.

Le Goff E, Martinand-Mari C, Martin M, Feuillard J, Boublik Y, Godefroy N, Mangeat P, Baghdiguian S, Cavalli G - Biol Open (2015)

Bottom Line: These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark.As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated.Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner.

View Article: PubMed Central - PubMed

Affiliation: Université Montpellier, Place Eugène Bataillon, Montpellier 34095, Cedex 5, France Institut des Sciences de l'Evolution (ISEM), UMR5554, CNRS, Montpellier 34095, France.

No MeSH data available.


Related in: MedlinePlus

. mRNA expression of tissue-specific genes and Hox12 gene in wild-type embryos and Ci-E(z) morphants. Time course of muscle (Macho1, Tbx6c), nervous system (ETR), notochord (Noto4), epiderm (Epi1) specific and Hox12 mRNA expression between 1-cell to hatching stage in wild-type embryos (dark grey) and Ci-E(z) morphants (light grey). The histograms are the mean of four independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the highest amount of wild-type target mRNA except for Hox12 and Epi1 mRNA expression for which it corresponds to the highest amount of Ci-E(z) morphant target mRNA. P values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from four independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4582116&req=5

BIO010835F8: . mRNA expression of tissue-specific genes and Hox12 gene in wild-type embryos and Ci-E(z) morphants. Time course of muscle (Macho1, Tbx6c), nervous system (ETR), notochord (Noto4), epiderm (Epi1) specific and Hox12 mRNA expression between 1-cell to hatching stage in wild-type embryos (dark grey) and Ci-E(z) morphants (light grey). The histograms are the mean of four independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the highest amount of wild-type target mRNA except for Hox12 and Epi1 mRNA expression for which it corresponds to the highest amount of Ci-E(z) morphant target mRNA. P values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from four independent experiments.

Mentions: To complement the description of the Ci-E(z) phenotype, we further analyzed by qPCR the expression of various genes known to be implicated in the formation of embryonic tissues and organs (Fig. 8). We chose genes involved in muscle (Macho1 and Tbx6c), nervous system (ETR), notochord (Noto4) and epidermal (Epi1) development as well as Hox12 gene, the only Hox gene whose its morpholino slightly modifies the embryo development phenotype (Ikuta et al., 2010). A first category encompasses three genes (Macho1, Tbx6c and ETR) whose expression was increased in morphant context, starting from the 4-cell stage. For Macho1 gene, its overall levels of expression declined in later development but with the significant maintenance of a relative difference between control and morphant. Tbx6c, a Macho1-induced gene (Yagi et al., 2005), was also overexpressed all along embryonic development up to hatching larval stage. In contrast, ETR overexpression was biphasic with a first peak up to 64-cell stage followed by down regulation at gastrula and a second phase of overexpression starting at late tailbud stage. It should be noticed that, in controls, the ETR expression peak was delayed to gastrula and neurula stages.Fig. 8


Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis.

Le Goff E, Martinand-Mari C, Martin M, Feuillard J, Boublik Y, Godefroy N, Mangeat P, Baghdiguian S, Cavalli G - Biol Open (2015)

. mRNA expression of tissue-specific genes and Hox12 gene in wild-type embryos and Ci-E(z) morphants. Time course of muscle (Macho1, Tbx6c), nervous system (ETR), notochord (Noto4), epiderm (Epi1) specific and Hox12 mRNA expression between 1-cell to hatching stage in wild-type embryos (dark grey) and Ci-E(z) morphants (light grey). The histograms are the mean of four independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the highest amount of wild-type target mRNA except for Hox12 and Epi1 mRNA expression for which it corresponds to the highest amount of Ci-E(z) morphant target mRNA. P values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from four independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582116&req=5

BIO010835F8: . mRNA expression of tissue-specific genes and Hox12 gene in wild-type embryos and Ci-E(z) morphants. Time course of muscle (Macho1, Tbx6c), nervous system (ETR), notochord (Noto4), epiderm (Epi1) specific and Hox12 mRNA expression between 1-cell to hatching stage in wild-type embryos (dark grey) and Ci-E(z) morphants (light grey). The histograms are the mean of four independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the highest amount of wild-type target mRNA except for Hox12 and Epi1 mRNA expression for which it corresponds to the highest amount of Ci-E(z) morphant target mRNA. P values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from four independent experiments.
Mentions: To complement the description of the Ci-E(z) phenotype, we further analyzed by qPCR the expression of various genes known to be implicated in the formation of embryonic tissues and organs (Fig. 8). We chose genes involved in muscle (Macho1 and Tbx6c), nervous system (ETR), notochord (Noto4) and epidermal (Epi1) development as well as Hox12 gene, the only Hox gene whose its morpholino slightly modifies the embryo development phenotype (Ikuta et al., 2010). A first category encompasses three genes (Macho1, Tbx6c and ETR) whose expression was increased in morphant context, starting from the 4-cell stage. For Macho1 gene, its overall levels of expression declined in later development but with the significant maintenance of a relative difference between control and morphant. Tbx6c, a Macho1-induced gene (Yagi et al., 2005), was also overexpressed all along embryonic development up to hatching larval stage. In contrast, ETR overexpression was biphasic with a first peak up to 64-cell stage followed by down regulation at gastrula and a second phase of overexpression starting at late tailbud stage. It should be noticed that, in controls, the ETR expression peak was delayed to gastrula and neurula stages.Fig. 8

Bottom Line: These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark.As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated.Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner.

View Article: PubMed Central - PubMed

Affiliation: Université Montpellier, Place Eugène Bataillon, Montpellier 34095, Cedex 5, France Institut des Sciences de l'Evolution (ISEM), UMR5554, CNRS, Montpellier 34095, France.

No MeSH data available.


Related in: MedlinePlus