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Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis.

Le Goff E, Martinand-Mari C, Martin M, Feuillard J, Boublik Y, Godefroy N, Mangeat P, Baghdiguian S, Cavalli G - Biol Open (2015)

Bottom Line: These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark.As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated.Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner.

View Article: PubMed Central - PubMed

Affiliation: Université Montpellier, Place Eugène Bataillon, Montpellier 34095, Cedex 5, France Institut des Sciences de l'Evolution (ISEM), UMR5554, CNRS, Montpellier 34095, France.

No MeSH data available.


Related in: MedlinePlus

. mRNA expression of tissue-specific genes in wild-type embryos, Ci-E(z) and Rescue morphants.Macho1, Tbx6c and ETR mRNA expression at 16-cell stage in wild-type embryos (dark grey), Ci-E(z) morphants (intermediate grey) and Rescue embryos (light grey). The histograms are the mean of two (Macho1) or three (Tbx6c and ETR) independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the amount of wild type target mRNA. P-values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from three independent experiments.
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BIO010835F10: . mRNA expression of tissue-specific genes in wild-type embryos, Ci-E(z) and Rescue morphants.Macho1, Tbx6c and ETR mRNA expression at 16-cell stage in wild-type embryos (dark grey), Ci-E(z) morphants (intermediate grey) and Rescue embryos (light grey). The histograms are the mean of two (Macho1) or three (Tbx6c and ETR) independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the amount of wild type target mRNA. P-values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from three independent experiments.

Mentions: In order to confirm the specificity of Ci-E(z) morpholino on genes presenting an early derepression (Macho1, Tbx6c and ETR), we tested by qPCR whether their 16-cell stage wild-type expression could be restored in the rescue experiment. As expected, the expression of the three genes was again suppressed, albeit with a lower efficiency for Tbx6c (Fig. 10).Fig. 10


Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis.

Le Goff E, Martinand-Mari C, Martin M, Feuillard J, Boublik Y, Godefroy N, Mangeat P, Baghdiguian S, Cavalli G - Biol Open (2015)

. mRNA expression of tissue-specific genes in wild-type embryos, Ci-E(z) and Rescue morphants.Macho1, Tbx6c and ETR mRNA expression at 16-cell stage in wild-type embryos (dark grey), Ci-E(z) morphants (intermediate grey) and Rescue embryos (light grey). The histograms are the mean of two (Macho1) or three (Tbx6c and ETR) independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the amount of wild type target mRNA. P-values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582116&req=5

BIO010835F10: . mRNA expression of tissue-specific genes in wild-type embryos, Ci-E(z) and Rescue morphants.Macho1, Tbx6c and ETR mRNA expression at 16-cell stage in wild-type embryos (dark grey), Ci-E(z) morphants (intermediate grey) and Rescue embryos (light grey). The histograms are the mean of two (Macho1) or three (Tbx6c and ETR) independent micro-injection experiments; data were normalized to respective S26 mRNA expression values. A relative mRNA quantity value of one corresponds to the amount of wild type target mRNA. P-values (*P<0.05) were calculated using the Wilcoxon rank sum test. Error bars correspond to the standard deviation from three independent experiments.
Mentions: In order to confirm the specificity of Ci-E(z) morpholino on genes presenting an early derepression (Macho1, Tbx6c and ETR), we tested by qPCR whether their 16-cell stage wild-type expression could be restored in the rescue experiment. As expected, the expression of the three genes was again suppressed, albeit with a lower efficiency for Tbx6c (Fig. 10).Fig. 10

Bottom Line: These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark.As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated.Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner.

View Article: PubMed Central - PubMed

Affiliation: Université Montpellier, Place Eugène Bataillon, Montpellier 34095, Cedex 5, France Institut des Sciences de l'Evolution (ISEM), UMR5554, CNRS, Montpellier 34095, France.

No MeSH data available.


Related in: MedlinePlus