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Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons.

Cortés-Campos C, Letelier J, Ceriani R, Whitlock KE - Biol Open (2015)

Bottom Line: Using this system, we show that neurospheres derived from the adult hypothalamus can be maintained in culture and subsequently differentiate glia and neurons.Finally, we show in vivo that a neurogenic niche in the hypothalamus contains GnRH positive neurons.Thus, we demonstrated for the first time that neurospheres can be derived from the hypothalamus of the adult zebrafish and that these neural progenitors are capable of producing GnRH containing neurons.

View Article: PubMed Central - PubMed

Affiliation: Centro Interdisciplinario de Neurociencia de Valparaíso (CINV), Facultad de Ciencias, Universidad de Valparaíso, Pasaje Harrington 269, Valparaíso 2340000, Chile Whitehead Institute for Biomedical Research (WIBR), 9 Cambridge Center, Cambridge, MA 02142, USA.

No MeSH data available.


Related in: MedlinePlus

GnRH antibodies recognize cells in the anterior preoptic region of the adult brain. Brain sections (A,B, cryostat sections; C-G, paraffin sections) of different animals immuno-stained with different antibodies against GnRH: (A,B) anti-GnRH (LRH13); (C,D) anti-GnRH (Hu11B); (E,F) anti-mGnRH (Sigma-Aldrich); (G,H) anti-sGnRH (BB8). All antibodies show immunoreactivity in neurons (arrows) localized in the hypothalamic POA (diagram, red). Scale bars: 50 μm.
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BIO010447F1: GnRH antibodies recognize cells in the anterior preoptic region of the adult brain. Brain sections (A,B, cryostat sections; C-G, paraffin sections) of different animals immuno-stained with different antibodies against GnRH: (A,B) anti-GnRH (LRH13); (C,D) anti-GnRH (Hu11B); (E,F) anti-mGnRH (Sigma-Aldrich); (G,H) anti-sGnRH (BB8). All antibodies show immunoreactivity in neurons (arrows) localized in the hypothalamic POA (diagram, red). Scale bars: 50 μm.

Mentions: To analyze the expression of GnRH in the hypothalamus of the adult zebrafish we used different antibodies known to recognize hypothalamic GnRH in mammals and fish: anti-GnRH (LRH13); anti-GnRH (Hu11B); anti-mGnRH; anti-sGnRH (BB8). When used on sections of adult brains, these antibodies recognize a small population of cells in the POA (Fig. 1). This population of GnRH containing cells shows a consistent pattern of immuno-labeling in both cryostat and paraffin embedded sectioned tissue. This pattern does not overlap with that of either GnRH2 or GnRH3 gene expression (Gopinath et al., 2004) or that of transgenic reporter line expression (GnRH3:GFP; Abraham et al., 2008; Zhao et al., 2013), and therefore could correspond to a hypothalamic GnRH isoform in zebrafish. Thus we have confirmed the presence of GnRH in the parvocellular nucleus of the POA.Fig. 1.


Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons.

Cortés-Campos C, Letelier J, Ceriani R, Whitlock KE - Biol Open (2015)

GnRH antibodies recognize cells in the anterior preoptic region of the adult brain. Brain sections (A,B, cryostat sections; C-G, paraffin sections) of different animals immuno-stained with different antibodies against GnRH: (A,B) anti-GnRH (LRH13); (C,D) anti-GnRH (Hu11B); (E,F) anti-mGnRH (Sigma-Aldrich); (G,H) anti-sGnRH (BB8). All antibodies show immunoreactivity in neurons (arrows) localized in the hypothalamic POA (diagram, red). Scale bars: 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582115&req=5

BIO010447F1: GnRH antibodies recognize cells in the anterior preoptic region of the adult brain. Brain sections (A,B, cryostat sections; C-G, paraffin sections) of different animals immuno-stained with different antibodies against GnRH: (A,B) anti-GnRH (LRH13); (C,D) anti-GnRH (Hu11B); (E,F) anti-mGnRH (Sigma-Aldrich); (G,H) anti-sGnRH (BB8). All antibodies show immunoreactivity in neurons (arrows) localized in the hypothalamic POA (diagram, red). Scale bars: 50 μm.
Mentions: To analyze the expression of GnRH in the hypothalamus of the adult zebrafish we used different antibodies known to recognize hypothalamic GnRH in mammals and fish: anti-GnRH (LRH13); anti-GnRH (Hu11B); anti-mGnRH; anti-sGnRH (BB8). When used on sections of adult brains, these antibodies recognize a small population of cells in the POA (Fig. 1). This population of GnRH containing cells shows a consistent pattern of immuno-labeling in both cryostat and paraffin embedded sectioned tissue. This pattern does not overlap with that of either GnRH2 or GnRH3 gene expression (Gopinath et al., 2004) or that of transgenic reporter line expression (GnRH3:GFP; Abraham et al., 2008; Zhao et al., 2013), and therefore could correspond to a hypothalamic GnRH isoform in zebrafish. Thus we have confirmed the presence of GnRH in the parvocellular nucleus of the POA.Fig. 1.

Bottom Line: Using this system, we show that neurospheres derived from the adult hypothalamus can be maintained in culture and subsequently differentiate glia and neurons.Finally, we show in vivo that a neurogenic niche in the hypothalamus contains GnRH positive neurons.Thus, we demonstrated for the first time that neurospheres can be derived from the hypothalamus of the adult zebrafish and that these neural progenitors are capable of producing GnRH containing neurons.

View Article: PubMed Central - PubMed

Affiliation: Centro Interdisciplinario de Neurociencia de Valparaíso (CINV), Facultad de Ciencias, Universidad de Valparaíso, Pasaje Harrington 269, Valparaíso 2340000, Chile Whitehead Institute for Biomedical Research (WIBR), 9 Cambridge Center, Cambridge, MA 02142, USA.

No MeSH data available.


Related in: MedlinePlus