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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

When grafted in the first branchial arch (BA1) presumptive region, hADSC migrated as neural crest cells. (A,F,H) Coronal sections of E4.5 chick embryos. Scale bar: 500 µm. ov., otic vesicle. (B,B′,C) The hADSC found in BA1 derivatives were in the mandibular bud (md.), associated with HNK1+ nerves. Red outlines: location of Alu+ nuclei in serial sections. (D,D′,E) Few hADSC were observed around the thyroid bud (thy.). (G,G′) Most of the hADSC migrated alongside cardiac neural crest cells and were found in the truncus arteriosus (t.a.). hADSC were in the cardiac jelly, between the endocardium (endo.) and myocardium (myo.). (I,I′) It was possible to observe hADSC dispersed from the second branchial arch (b.a.2) into the t.a. B,D,G,I, scale bar: 200 µm; B′,C,D′,E,G′,I′, Scale bar: 20 µm. b.a.3, third branchial arch; mx., maxillary bud; ph., pharynx.
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BIO010256F7: When grafted in the first branchial arch (BA1) presumptive region, hADSC migrated as neural crest cells. (A,F,H) Coronal sections of E4.5 chick embryos. Scale bar: 500 µm. ov., otic vesicle. (B,B′,C) The hADSC found in BA1 derivatives were in the mandibular bud (md.), associated with HNK1+ nerves. Red outlines: location of Alu+ nuclei in serial sections. (D,D′,E) Few hADSC were observed around the thyroid bud (thy.). (G,G′) Most of the hADSC migrated alongside cardiac neural crest cells and were found in the truncus arteriosus (t.a.). hADSC were in the cardiac jelly, between the endocardium (endo.) and myocardium (myo.). (I,I′) It was possible to observe hADSC dispersed from the second branchial arch (b.a.2) into the t.a. B,D,G,I, scale bar: 200 µm; B′,C,D′,E,G′,I′, Scale bar: 20 µm. b.a.3, third branchial arch; mx., maxillary bud; ph., pharynx.

Mentions: Part of the hADSC was found in a BA1 derivative, the mandibular bud (21.4%) (n=2/3) (Fig. 7A,B). Alu+ cells were not dispersed through the mesenchyme; HNK1 immunostaining in adjacent sections revealed that human cells were associated with cranial nerves (Fig. 7B′,C), in a behavior similar to the hADSC grafted into the trunk.Fig. 7.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

When grafted in the first branchial arch (BA1) presumptive region, hADSC migrated as neural crest cells. (A,F,H) Coronal sections of E4.5 chick embryos. Scale bar: 500 µm. ov., otic vesicle. (B,B′,C) The hADSC found in BA1 derivatives were in the mandibular bud (md.), associated with HNK1+ nerves. Red outlines: location of Alu+ nuclei in serial sections. (D,D′,E) Few hADSC were observed around the thyroid bud (thy.). (G,G′) Most of the hADSC migrated alongside cardiac neural crest cells and were found in the truncus arteriosus (t.a.). hADSC were in the cardiac jelly, between the endocardium (endo.) and myocardium (myo.). (I,I′) It was possible to observe hADSC dispersed from the second branchial arch (b.a.2) into the t.a. B,D,G,I, scale bar: 200 µm; B′,C,D′,E,G′,I′, Scale bar: 20 µm. b.a.3, third branchial arch; mx., maxillary bud; ph., pharynx.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F7: When grafted in the first branchial arch (BA1) presumptive region, hADSC migrated as neural crest cells. (A,F,H) Coronal sections of E4.5 chick embryos. Scale bar: 500 µm. ov., otic vesicle. (B,B′,C) The hADSC found in BA1 derivatives were in the mandibular bud (md.), associated with HNK1+ nerves. Red outlines: location of Alu+ nuclei in serial sections. (D,D′,E) Few hADSC were observed around the thyroid bud (thy.). (G,G′) Most of the hADSC migrated alongside cardiac neural crest cells and were found in the truncus arteriosus (t.a.). hADSC were in the cardiac jelly, between the endocardium (endo.) and myocardium (myo.). (I,I′) It was possible to observe hADSC dispersed from the second branchial arch (b.a.2) into the t.a. B,D,G,I, scale bar: 200 µm; B′,C,D′,E,G′,I′, Scale bar: 20 µm. b.a.3, third branchial arch; mx., maxillary bud; ph., pharynx.
Mentions: Part of the hADSC was found in a BA1 derivative, the mandibular bud (21.4%) (n=2/3) (Fig. 7A,B). Alu+ cells were not dispersed through the mesenchyme; HNK1 immunostaining in adjacent sections revealed that human cells were associated with cranial nerves (Fig. 7B′,C), in a behavior similar to the hADSC grafted into the trunk.Fig. 7.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus