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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

In E6 chick embryos, hSF revealed a smaller tropism for nerves than hADSC. (A) In situ hybridization with Alu probes and Alcian blue counterstaining in cross-sections at anterior limb bud level. hSF were dispersed throughout the mesenchyme (dark blue nuclei), including the dermis (gray arrow). (B) In an adjacent section immunostained with HNK1, the majority of hSF (red outlines) were not adjacent to peripheral nerves. A,B, scale bar: 100 µm. d.r.g., dorsal root ganglion; m., mesonephron.
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BIO010256F6: In E6 chick embryos, hSF revealed a smaller tropism for nerves than hADSC. (A) In situ hybridization with Alu probes and Alcian blue counterstaining in cross-sections at anterior limb bud level. hSF were dispersed throughout the mesenchyme (dark blue nuclei), including the dermis (gray arrow). (B) In an adjacent section immunostained with HNK1, the majority of hSF (red outlines) were not adjacent to peripheral nerves. A,B, scale bar: 100 µm. d.r.g., dorsal root ganglion; m., mesonephron.

Mentions: Spheroids of hSF were grafted into the presomitic mesoderm of E2 (15–19ss) chick embryos, and the cells were located using Alu probes. At E6, the hSFs were dispersed throughout the embryo (n=4), showing different distributions (Fig. 6). In contrast to hADSC, 5.1% of the hSFs were able to migrate into the mesenchyme of the dermis (n=4/4) (Fig. 6A, gray arrow), adopting a more elongated, fibroblast-like phenotype. Moreover, part of the hSF were observed dispersed throughout the mesenchyme, without either perivascular or perineural locations (44.2%, n=4/4). Similar percentages of hSF (16.2%, n=4/4) and hADSC (10.9%) were found in perivascular regions. However, only 43.8% (n=4/4, 80 of 235 cells) of the hSF were observed associated with HNK1+ tissues, in contrast to the 88.5% (n=6/6, 836 of 945 cells) of the hADSC that occupied this niche. No hSF appeared to be HNK1+. The distribution of hSF in E6 is described in supplementary material Table S1.Fig. 6.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

In E6 chick embryos, hSF revealed a smaller tropism for nerves than hADSC. (A) In situ hybridization with Alu probes and Alcian blue counterstaining in cross-sections at anterior limb bud level. hSF were dispersed throughout the mesenchyme (dark blue nuclei), including the dermis (gray arrow). (B) In an adjacent section immunostained with HNK1, the majority of hSF (red outlines) were not adjacent to peripheral nerves. A,B, scale bar: 100 µm. d.r.g., dorsal root ganglion; m., mesonephron.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F6: In E6 chick embryos, hSF revealed a smaller tropism for nerves than hADSC. (A) In situ hybridization with Alu probes and Alcian blue counterstaining in cross-sections at anterior limb bud level. hSF were dispersed throughout the mesenchyme (dark blue nuclei), including the dermis (gray arrow). (B) In an adjacent section immunostained with HNK1, the majority of hSF (red outlines) were not adjacent to peripheral nerves. A,B, scale bar: 100 µm. d.r.g., dorsal root ganglion; m., mesonephron.
Mentions: Spheroids of hSF were grafted into the presomitic mesoderm of E2 (15–19ss) chick embryos, and the cells were located using Alu probes. At E6, the hSFs were dispersed throughout the embryo (n=4), showing different distributions (Fig. 6). In contrast to hADSC, 5.1% of the hSFs were able to migrate into the mesenchyme of the dermis (n=4/4) (Fig. 6A, gray arrow), adopting a more elongated, fibroblast-like phenotype. Moreover, part of the hSF were observed dispersed throughout the mesenchyme, without either perivascular or perineural locations (44.2%, n=4/4). Similar percentages of hSF (16.2%, n=4/4) and hADSC (10.9%) were found in perivascular regions. However, only 43.8% (n=4/4, 80 of 235 cells) of the hSF were observed associated with HNK1+ tissues, in contrast to the 88.5% (n=6/6, 836 of 945 cells) of the hADSC that occupied this niche. No hSF appeared to be HNK1+. The distribution of hSF in E6 is described in supplementary material Table S1.Fig. 6.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus