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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

In E6 chick embryos, hADSC migrated associating with peripheral nerves and also formed blood vessels.In situ hybridization with Alu probes, immunostaining with HNK1 antibody and Alcian blue counterstaining in cross-sections at the anterior limb bud level. (A,A′) When hADSC were grafted on the level of the aortic plexus (a.p.), human cells were found ventrally until the dorsal aorta (d.a.), associated with HNK1+. A few cells presented a HNK1+ cytoplasm (A″, green arrows), although most of them were HNK1− (A‴). (B) hADSC were located in the d.a. wall. Note other cells lateral to the neural tube (n.t.) and in the ventralmost region of the d.a. (black arrows). (B′,B″,B‴) Human cells were perivascular, while the endothelium was chicken-derived (red arrows). (C,C′) hADSC were found in the mesonephros (m.). (C″) Human cells were in contact with the basal lamina of the Wolffian channels (w.c.), in a perivascular location. Again, the endothelium was derived from chicken. A,B,C, scale bar: 200 µm; A′,B′,C′, scale bar: 50 µm; A″,A‴,B″,B‴,C″, scale bar: 10 µm. c., coelom; gl., glomerulus; v., blood vessel.
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BIO010256F5: In E6 chick embryos, hADSC migrated associating with peripheral nerves and also formed blood vessels.In situ hybridization with Alu probes, immunostaining with HNK1 antibody and Alcian blue counterstaining in cross-sections at the anterior limb bud level. (A,A′) When hADSC were grafted on the level of the aortic plexus (a.p.), human cells were found ventrally until the dorsal aorta (d.a.), associated with HNK1+. A few cells presented a HNK1+ cytoplasm (A″, green arrows), although most of them were HNK1− (A‴). (B) hADSC were located in the d.a. wall. Note other cells lateral to the neural tube (n.t.) and in the ventralmost region of the d.a. (black arrows). (B′,B″,B‴) Human cells were perivascular, while the endothelium was chicken-derived (red arrows). (C,C′) hADSC were found in the mesonephros (m.). (C″) Human cells were in contact with the basal lamina of the Wolffian channels (w.c.), in a perivascular location. Again, the endothelium was derived from chicken. A,B,C, scale bar: 200 µm; A′,B′,C′, scale bar: 50 µm; A″,A‴,B″,B‴,C″, scale bar: 10 µm. c., coelom; gl., glomerulus; v., blood vessel.

Mentions: In levels where the nerves extended farther than the neural tube and the sympathetic chain, the hADSC had the capacity to migrate along these nerves into additional regions of the embryo (n=4/6). The distribution of Alu+ cells from the dorsal region of the neural tube to the mesonephros and ventral side of the dorsal-aortic wall is shown in a series of cross-sections (Fig. 5A,B,C, black arrows). The more-ventral distribution of human cells was closely associated with the aortic plexus, as shown by the association of Alu+ cells with HNK1+ nerves extending to the dorsal aortic walls (Fig. 5A′,B′). Reinforcing the notion that hADSC distribution could be related to peripheral nerves and blood vessels, in cross-sections where the aortic plexus was not observed, Alu+ cells remained more dorsal, at the level of the sympathetic chain (Fig. 4C,C′) (n=2/6).Fig. 5.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

In E6 chick embryos, hADSC migrated associating with peripheral nerves and also formed blood vessels.In situ hybridization with Alu probes, immunostaining with HNK1 antibody and Alcian blue counterstaining in cross-sections at the anterior limb bud level. (A,A′) When hADSC were grafted on the level of the aortic plexus (a.p.), human cells were found ventrally until the dorsal aorta (d.a.), associated with HNK1+. A few cells presented a HNK1+ cytoplasm (A″, green arrows), although most of them were HNK1− (A‴). (B) hADSC were located in the d.a. wall. Note other cells lateral to the neural tube (n.t.) and in the ventralmost region of the d.a. (black arrows). (B′,B″,B‴) Human cells were perivascular, while the endothelium was chicken-derived (red arrows). (C,C′) hADSC were found in the mesonephros (m.). (C″) Human cells were in contact with the basal lamina of the Wolffian channels (w.c.), in a perivascular location. Again, the endothelium was derived from chicken. A,B,C, scale bar: 200 µm; A′,B′,C′, scale bar: 50 µm; A″,A‴,B″,B‴,C″, scale bar: 10 µm. c., coelom; gl., glomerulus; v., blood vessel.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F5: In E6 chick embryos, hADSC migrated associating with peripheral nerves and also formed blood vessels.In situ hybridization with Alu probes, immunostaining with HNK1 antibody and Alcian blue counterstaining in cross-sections at the anterior limb bud level. (A,A′) When hADSC were grafted on the level of the aortic plexus (a.p.), human cells were found ventrally until the dorsal aorta (d.a.), associated with HNK1+. A few cells presented a HNK1+ cytoplasm (A″, green arrows), although most of them were HNK1− (A‴). (B) hADSC were located in the d.a. wall. Note other cells lateral to the neural tube (n.t.) and in the ventralmost region of the d.a. (black arrows). (B′,B″,B‴) Human cells were perivascular, while the endothelium was chicken-derived (red arrows). (C,C′) hADSC were found in the mesonephros (m.). (C″) Human cells were in contact with the basal lamina of the Wolffian channels (w.c.), in a perivascular location. Again, the endothelium was derived from chicken. A,B,C, scale bar: 200 µm; A′,B′,C′, scale bar: 50 µm; A″,A‴,B″,B‴,C″, scale bar: 10 µm. c., coelom; gl., glomerulus; v., blood vessel.
Mentions: In levels where the nerves extended farther than the neural tube and the sympathetic chain, the hADSC had the capacity to migrate along these nerves into additional regions of the embryo (n=4/6). The distribution of Alu+ cells from the dorsal region of the neural tube to the mesonephros and ventral side of the dorsal-aortic wall is shown in a series of cross-sections (Fig. 5A,B,C, black arrows). The more-ventral distribution of human cells was closely associated with the aortic plexus, as shown by the association of Alu+ cells with HNK1+ nerves extending to the dorsal aortic walls (Fig. 5A′,B′). Reinforcing the notion that hADSC distribution could be related to peripheral nerves and blood vessels, in cross-sections where the aortic plexus was not observed, Alu+ cells remained more dorsal, at the level of the sympathetic chain (Fig. 4C,C′) (n=2/6).Fig. 5.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus