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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

In E6 chick embryos, hADSC associated with peripheral nerves and ganglia. (A,A′) Cross-sections at anterior limb-bud (l.b.) level revealed that the morphology of the embryo is normal. Haematoxylin-eosin (HE) staining. (B,B′,B″) In serial sections, hybridization with Alu probes performed simultaneously with HNK1 immunostaining revealed that hADSC were closely associated with developing nerve fibers, lateral to the neural tube (n.t.), in the region of the dorsal-root ganglion (d.r.g.). Alcian blue counterstaining. (C,C′) hADSC were associated with the sympathetic ganglia (s.g.). (D) Cells were also associated with blood vessels, always in a perivascular location, as seen with HE staining. Red arrows: endothelium. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm; B′,B″,C′, scale bar: 10 µm.
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BIO010256F4: In E6 chick embryos, hADSC associated with peripheral nerves and ganglia. (A,A′) Cross-sections at anterior limb-bud (l.b.) level revealed that the morphology of the embryo is normal. Haematoxylin-eosin (HE) staining. (B,B′,B″) In serial sections, hybridization with Alu probes performed simultaneously with HNK1 immunostaining revealed that hADSC were closely associated with developing nerve fibers, lateral to the neural tube (n.t.), in the region of the dorsal-root ganglion (d.r.g.). Alcian blue counterstaining. (C,C′) hADSC were associated with the sympathetic ganglia (s.g.). (D) Cells were also associated with blood vessels, always in a perivascular location, as seen with HE staining. Red arrows: endothelium. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm; B′,B″,C′, scale bar: 10 µm.

Mentions: Given the unexpected results described above, it was necessary to characterize the distribution of hADSC and whether they interacted with specific cell types of the chick embryo. First, cross-sections hybridized with Alu probes were compared with adjacent sections stained with haematoxylin-eosin (HE). E6 embryos had normal morphology (Fig. 4A). In Alu-hybridized sections, positive cells were observed lateral to the neural tube in the region of the dorsal-root ganglia and sympathetic chain (Fig. 4A′,B, brackets).Fig. 4.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

In E6 chick embryos, hADSC associated with peripheral nerves and ganglia. (A,A′) Cross-sections at anterior limb-bud (l.b.) level revealed that the morphology of the embryo is normal. Haematoxylin-eosin (HE) staining. (B,B′,B″) In serial sections, hybridization with Alu probes performed simultaneously with HNK1 immunostaining revealed that hADSC were closely associated with developing nerve fibers, lateral to the neural tube (n.t.), in the region of the dorsal-root ganglion (d.r.g.). Alcian blue counterstaining. (C,C′) hADSC were associated with the sympathetic ganglia (s.g.). (D) Cells were also associated with blood vessels, always in a perivascular location, as seen with HE staining. Red arrows: endothelium. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm; B′,B″,C′, scale bar: 10 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F4: In E6 chick embryos, hADSC associated with peripheral nerves and ganglia. (A,A′) Cross-sections at anterior limb-bud (l.b.) level revealed that the morphology of the embryo is normal. Haematoxylin-eosin (HE) staining. (B,B′,B″) In serial sections, hybridization with Alu probes performed simultaneously with HNK1 immunostaining revealed that hADSC were closely associated with developing nerve fibers, lateral to the neural tube (n.t.), in the region of the dorsal-root ganglion (d.r.g.). Alcian blue counterstaining. (C,C′) hADSC were associated with the sympathetic ganglia (s.g.). (D) Cells were also associated with blood vessels, always in a perivascular location, as seen with HE staining. Red arrows: endothelium. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm; B′,B″,C′, scale bar: 10 µm.
Mentions: Given the unexpected results described above, it was necessary to characterize the distribution of hADSC and whether they interacted with specific cell types of the chick embryo. First, cross-sections hybridized with Alu probes were compared with adjacent sections stained with haematoxylin-eosin (HE). E6 embryos had normal morphology (Fig. 4A). In Alu-hybridized sections, positive cells were observed lateral to the neural tube in the region of the dorsal-root ganglia and sympathetic chain (Fig. 4A′,B, brackets).Fig. 4.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus