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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

hADSC were not localized in osteogenic or chondrogenic regions in E6 chick embryos. (A,A′) Cross-sections at the level of the anterior limb bud hybridized with Alu probes revealed a group of human cells lateral to the vertebral condensation. The nerve was revealed with HNK1 immunostaining. (B,C) In situ hybridization with chicken RNA probes in sections serial to (A′). hADSC (red outlines) were not found in chondrogenic (B) (Sox9+) or osteogenic (C) (Runx2+) regions. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm. n.t., neural tube.
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BIO010256F3: hADSC were not localized in osteogenic or chondrogenic regions in E6 chick embryos. (A,A′) Cross-sections at the level of the anterior limb bud hybridized with Alu probes revealed a group of human cells lateral to the vertebral condensation. The nerve was revealed with HNK1 immunostaining. (B,C) In situ hybridization with chicken RNA probes in sections serial to (A′). hADSC (red outlines) were not found in chondrogenic (B) (Sox9+) or osteogenic (C) (Runx2+) regions. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm. n.t., neural tube.

Mentions: At E6, the advanced stage of organogenesis allowed us to investigate the behavior of hADSC regarding committed territories and differentiating tissues. It has been reported that hADSC can differentiate into skeletal derivatives in vitro (Zuk et al., 2001) and in vivo (James et al., 2012b; Lendeckel et al., 2004; Mesimäki et al., 2009; Zheng et al., 2006). Therefore, we analyzed whether hADSC would be located in chick territories committed to chondrogenesis and osteogenesis. We also investigated whether hADSC would be found in myogenic territories, another mesoderm-derived tissue. In situ hybridization with Alu probes was performed in parallel with Alcian blue staining, to reveal cartilage; and in situ hybridization with Sox9, Runx2 and MyoD probes for chick RNA was performed to identify, respectively, cartilage-, bone- and muscle-committed territories of the host (n=3) (Fig. 3).Fig. 3.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

hADSC were not localized in osteogenic or chondrogenic regions in E6 chick embryos. (A,A′) Cross-sections at the level of the anterior limb bud hybridized with Alu probes revealed a group of human cells lateral to the vertebral condensation. The nerve was revealed with HNK1 immunostaining. (B,C) In situ hybridization with chicken RNA probes in sections serial to (A′). hADSC (red outlines) were not found in chondrogenic (B) (Sox9+) or osteogenic (C) (Runx2+) regions. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm. n.t., neural tube.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F3: hADSC were not localized in osteogenic or chondrogenic regions in E6 chick embryos. (A,A′) Cross-sections at the level of the anterior limb bud hybridized with Alu probes revealed a group of human cells lateral to the vertebral condensation. The nerve was revealed with HNK1 immunostaining. (B,C) In situ hybridization with chicken RNA probes in sections serial to (A′). hADSC (red outlines) were not found in chondrogenic (B) (Sox9+) or osteogenic (C) (Runx2+) regions. A, scale bar: 1 mm; A′,B,C, scale bar: 100 µm. n.t., neural tube.
Mentions: At E6, the advanced stage of organogenesis allowed us to investigate the behavior of hADSC regarding committed territories and differentiating tissues. It has been reported that hADSC can differentiate into skeletal derivatives in vitro (Zuk et al., 2001) and in vivo (James et al., 2012b; Lendeckel et al., 2004; Mesimäki et al., 2009; Zheng et al., 2006). Therefore, we analyzed whether hADSC would be located in chick territories committed to chondrogenesis and osteogenesis. We also investigated whether hADSC would be found in myogenic territories, another mesoderm-derived tissue. In situ hybridization with Alu probes was performed in parallel with Alcian blue staining, to reveal cartilage; and in situ hybridization with Sox9, Runx2 and MyoD probes for chick RNA was performed to identify, respectively, cartilage-, bone- and muscle-committed territories of the host (n=3) (Fig. 3).Fig. 3.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus