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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

Localization of hADSC in E3.5 chick embryos. (A) Nile blue sulfate staining revealed that, in the operated region (black arrow), there was no increase in cell death or apparent malformations in the embryo. Cell death is represented by the blue puncta, as observed in the mesonephros (asterisk). (B,C,D) Cross-sections at the anterior limb bud level of the embryo in A. Sections were hybridized with Alu probes and immunostained with HNK1 (migratory NCC). The spheroid was no longer visible, and human cells integrated with the embryo. (B) Human nuclei were observed in the mesenchyme from the neural tube until the mesonephros. (C,C′) Some human cells mingled with the NCC migration stream. (D) Cells observed in the mesonephros and dorsal aorta (d.a.) did not associate with NCC at this stage. (D′) hADSC associated with the d.a. were always in a perivascular position. The endothelium was derived from chicken (red arrow). B,C,D, scale bar: 50 µm; C′,D′, scale bar: 10 µm. l.b., wing limb bud; d.m., dorsal mesentery; n.d., nephric duct.
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BIO010256F2: Localization of hADSC in E3.5 chick embryos. (A) Nile blue sulfate staining revealed that, in the operated region (black arrow), there was no increase in cell death or apparent malformations in the embryo. Cell death is represented by the blue puncta, as observed in the mesonephros (asterisk). (B,C,D) Cross-sections at the anterior limb bud level of the embryo in A. Sections were hybridized with Alu probes and immunostained with HNK1 (migratory NCC). The spheroid was no longer visible, and human cells integrated with the embryo. (B) Human nuclei were observed in the mesenchyme from the neural tube until the mesonephros. (C,C′) Some human cells mingled with the NCC migration stream. (D) Cells observed in the mesonephros and dorsal aorta (d.a.) did not associate with NCC at this stage. (D′) hADSC associated with the d.a. were always in a perivascular position. The endothelium was derived from chicken (red arrow). B,C,D, scale bar: 50 µm; C′,D′, scale bar: 10 µm. l.b., wing limb bud; d.m., dorsal mesentery; n.d., nephric duct.

Mentions: First, cell death was investigated using Nile blue sulfate, a vital stain (Jeffs and Osmond, 1992). No cell death beyond the levels reported for normal development (Hirata and Hall, 2000; Jeffs and Osmond, 1992) was observed at E3.5 (n=3) (Fig. 2A). Few cells were stained with Nile blue sulfate in the vicinity of the graft (Fig. 2A, black arrow), suggesting that the surgery and the grafted spheroids did not induce cell death in the tissues of the chick embryo.Fig. 2.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Localization of hADSC in E3.5 chick embryos. (A) Nile blue sulfate staining revealed that, in the operated region (black arrow), there was no increase in cell death or apparent malformations in the embryo. Cell death is represented by the blue puncta, as observed in the mesonephros (asterisk). (B,C,D) Cross-sections at the anterior limb bud level of the embryo in A. Sections were hybridized with Alu probes and immunostained with HNK1 (migratory NCC). The spheroid was no longer visible, and human cells integrated with the embryo. (B) Human nuclei were observed in the mesenchyme from the neural tube until the mesonephros. (C,C′) Some human cells mingled with the NCC migration stream. (D) Cells observed in the mesonephros and dorsal aorta (d.a.) did not associate with NCC at this stage. (D′) hADSC associated with the d.a. were always in a perivascular position. The endothelium was derived from chicken (red arrow). B,C,D, scale bar: 50 µm; C′,D′, scale bar: 10 µm. l.b., wing limb bud; d.m., dorsal mesentery; n.d., nephric duct.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F2: Localization of hADSC in E3.5 chick embryos. (A) Nile blue sulfate staining revealed that, in the operated region (black arrow), there was no increase in cell death or apparent malformations in the embryo. Cell death is represented by the blue puncta, as observed in the mesonephros (asterisk). (B,C,D) Cross-sections at the anterior limb bud level of the embryo in A. Sections were hybridized with Alu probes and immunostained with HNK1 (migratory NCC). The spheroid was no longer visible, and human cells integrated with the embryo. (B) Human nuclei were observed in the mesenchyme from the neural tube until the mesonephros. (C,C′) Some human cells mingled with the NCC migration stream. (D) Cells observed in the mesonephros and dorsal aorta (d.a.) did not associate with NCC at this stage. (D′) hADSC associated with the d.a. were always in a perivascular position. The endothelium was derived from chicken (red arrow). B,C,D, scale bar: 50 µm; C′,D′, scale bar: 10 µm. l.b., wing limb bud; d.m., dorsal mesentery; n.d., nephric duct.
Mentions: First, cell death was investigated using Nile blue sulfate, a vital stain (Jeffs and Osmond, 1992). No cell death beyond the levels reported for normal development (Hirata and Hall, 2000; Jeffs and Osmond, 1992) was observed at E3.5 (n=3) (Fig. 2A). Few cells were stained with Nile blue sulfate in the vicinity of the graft (Fig. 2A, black arrow), suggesting that the surgery and the grafted spheroids did not induce cell death in the tissues of the chick embryo.Fig. 2.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus