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Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus

Grafting hADSC in 2-day chick embryos. (A) Spheroid suitable for grafting. Vertical line: 131.4 µm; horizontal line: 130.8 µm. (B) 14ss embryo with a spheroid grafted at presumptive 17th somite level. (C) Cross-section of the embryo in B (dashed line), 4 h after surgery. In situ hybridization with human Alu probes. The hADSC spheroid did not affect the formation of chick tissues. Scale bar: 100 µm. (C′) Only human nuclei (black arrow) were stained after in situ hybridization; chick nuclei (blue arrow) and human cytoplasm were unstained. Scale bar: 10 µm. i.m., intermediate mesoderm; n.t., neural tube; s., somite.
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BIO010256F1: Grafting hADSC in 2-day chick embryos. (A) Spheroid suitable for grafting. Vertical line: 131.4 µm; horizontal line: 130.8 µm. (B) 14ss embryo with a spheroid grafted at presumptive 17th somite level. (C) Cross-section of the embryo in B (dashed line), 4 h after surgery. In situ hybridization with human Alu probes. The hADSC spheroid did not affect the formation of chick tissues. Scale bar: 100 µm. (C′) Only human nuclei (black arrow) were stained after in situ hybridization; chick nuclei (blue arrow) and human cytoplasm were unstained. Scale bar: 10 µm. i.m., intermediate mesoderm; n.t., neural tube; s., somite.

Mentions: In order for xenografting of human cells into chick embryos to serve as an adequate model, it must not disrupt the host's morphogenesis and it must allow localizing a single donor cell. Spheroids were grafted instead of injecting a cell suspension into the embryo, which ensured precise placement of the graft and allowed us to investigate cell migration. hADSC spheroids were prepared 2 days before the surgery (Brito et al., 2008), yielding spheroids of numerous sizes. Spheroids with the approximate size of a somite were chosen by visual inspection. They contained a mean of 52.3 cells (n=10) and had a mean diameter of 135 μm (n=5) (Fig. 1A). Embryos developed until somite stage (ss) 13–19 [Hamburger and Hamilton (HH) stage 11–12; Hamburger and Hamilton, 1951], when a spheroid was grafted laterally to the neural tube in the presomitic mesoderm at the wing-bud level (presumptive somites 15–21) (Fig. 1B).Fig. 1.


Chick embryo xenograft model reveals a novel perineural niche for human adipose-derived stromal cells.

Cordeiro IR, Lopes DV, Abreu JG, Carneiro K, Rossi MI, Brito JM - Biol Open (2015)

Grafting hADSC in 2-day chick embryos. (A) Spheroid suitable for grafting. Vertical line: 131.4 µm; horizontal line: 130.8 µm. (B) 14ss embryo with a spheroid grafted at presumptive 17th somite level. (C) Cross-section of the embryo in B (dashed line), 4 h after surgery. In situ hybridization with human Alu probes. The hADSC spheroid did not affect the formation of chick tissues. Scale bar: 100 µm. (C′) Only human nuclei (black arrow) were stained after in situ hybridization; chick nuclei (blue arrow) and human cytoplasm were unstained. Scale bar: 10 µm. i.m., intermediate mesoderm; n.t., neural tube; s., somite.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582113&req=5

BIO010256F1: Grafting hADSC in 2-day chick embryos. (A) Spheroid suitable for grafting. Vertical line: 131.4 µm; horizontal line: 130.8 µm. (B) 14ss embryo with a spheroid grafted at presumptive 17th somite level. (C) Cross-section of the embryo in B (dashed line), 4 h after surgery. In situ hybridization with human Alu probes. The hADSC spheroid did not affect the formation of chick tissues. Scale bar: 100 µm. (C′) Only human nuclei (black arrow) were stained after in situ hybridization; chick nuclei (blue arrow) and human cytoplasm were unstained. Scale bar: 10 µm. i.m., intermediate mesoderm; n.t., neural tube; s., somite.
Mentions: In order for xenografting of human cells into chick embryos to serve as an adequate model, it must not disrupt the host's morphogenesis and it must allow localizing a single donor cell. Spheroids were grafted instead of injecting a cell suspension into the embryo, which ensured precise placement of the graft and allowed us to investigate cell migration. hADSC spheroids were prepared 2 days before the surgery (Brito et al., 2008), yielding spheroids of numerous sizes. Spheroids with the approximate size of a somite were chosen by visual inspection. They contained a mean of 52.3 cells (n=10) and had a mean diameter of 135 μm (n=5) (Fig. 1A). Embryos developed until somite stage (ss) 13–19 [Hamburger and Hamilton (HH) stage 11–12; Hamburger and Hamilton, 1951], when a spheroid was grafted laterally to the neural tube in the presomitic mesoderm at the wing-bud level (presumptive somites 15–21) (Fig. 1B).Fig. 1.

Bottom Line: When grafted into the trunk, in contrast to previous studies, hADSC were not found in chondrogenic or osteogenic territories up to E8.Surprisingly, 82.5% of the hADSC were associated with HNK1+ tissues, such as peripheral nerves.Therefore, we propose that xenografts of human cells into chick embryos can reveal novel behaviors of heterogeneous cell populations, such as response to migration cues.

View Article: PubMed Central - PubMed

Affiliation: Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.

No MeSH data available.


Related in: MedlinePlus