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Ovarian senescence increases liver fibrosis in humans and zebrafish with steatosis.

Turola E, Petta S, Vanni E, Milosa F, Valenti L, Critelli R, Miele L, Maccio L, Calvaruso V, Fracanzani AL, Bianchini M, Raos N, Bugianesi E, Mercorella S, Di Giovanni M, Craxì A, Fargion S, Grieco A, Cammà C, Cotelli F, Villa E - Dis Model Mech (2015)

Bottom Line: In the entire cohort, at multivariate logistic regression, male gender [odds ratio (OR): 1.408, 95% confidence interval (95% CI): 0.779-2.542, P=0.25] vs women at reproductive age was not associated with F2-F4 fibrosis, whereas a trend was observed for menopause (OR: 1.752, 95% CI: 0.956-3.208, P=0.06).In women, menopause (OR: 2.717, 95% CI: 1.020-7.237, P=0.04) was independently associated with F2-F4 fibrosis.Similarly, in overfed zebrafish, old female fish with failing ovarian function [as demonstrated by extremely low circulating estradiol levels (1.4±0.1 pg/µl) and prevailing presence of atretic follicles in the ovaries] developed massive steatosis and substantial fibrosis (comparable with that occurring in males), whereas young female fish developed less steatosis and were totally protected from the development of fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Gastroenterology Unit, Department of Internal Medicine, University of Modena and Reggio Emilia, 41124 Modena, Italy.

No MeSH data available.


Related in: MedlinePlus

Definition of reproductive age in zebrafish. (A) Serum estradiol levels in female and male zebrafish during ageing. Data are reported as mean±s.d. Final sample number=12 per sex/age. (B) Application of the ovary maturation classification in the evaluation of the effects of ageing on zebrafish ovarian tissue (see supplementary material Fig. S1 for details). Three sections from five fish per subgroup were analyzed. Data were expressed as mean±s.d. H&E-stained (C,E) and PAS-stained (D,F) sections of the ovaries of young (4 mpf) and old (18 mpf) female zebrafish. Atretic follicles (indicated by arrows) were mostly found in old female fish with low to absent E2 levels. mpf, months post fertilization.
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DMM019950F1: Definition of reproductive age in zebrafish. (A) Serum estradiol levels in female and male zebrafish during ageing. Data are reported as mean±s.d. Final sample number=12 per sex/age. (B) Application of the ovary maturation classification in the evaluation of the effects of ageing on zebrafish ovarian tissue (see supplementary material Fig. S1 for details). Three sections from five fish per subgroup were analyzed. Data were expressed as mean±s.d. H&E-stained (C,E) and PAS-stained (D,F) sections of the ovaries of young (4 mpf) and old (18 mpf) female zebrafish. Atretic follicles (indicated by arrows) were mostly found in old female fish with low to absent E2 levels. mpf, months post fertilization.

Mentions: In order to study the effect of diet at different reproductive ages, we used young and old zebrafish. To substantiate the reproductive age, in particular the ovarian senescence in old female fish, we analyzed the circulating levels of estradiol and performed histopathologic evaluation of ovaries (Fig. 1).Fig. 1.


Ovarian senescence increases liver fibrosis in humans and zebrafish with steatosis.

Turola E, Petta S, Vanni E, Milosa F, Valenti L, Critelli R, Miele L, Maccio L, Calvaruso V, Fracanzani AL, Bianchini M, Raos N, Bugianesi E, Mercorella S, Di Giovanni M, Craxì A, Fargion S, Grieco A, Cammà C, Cotelli F, Villa E - Dis Model Mech (2015)

Definition of reproductive age in zebrafish. (A) Serum estradiol levels in female and male zebrafish during ageing. Data are reported as mean±s.d. Final sample number=12 per sex/age. (B) Application of the ovary maturation classification in the evaluation of the effects of ageing on zebrafish ovarian tissue (see supplementary material Fig. S1 for details). Three sections from five fish per subgroup were analyzed. Data were expressed as mean±s.d. H&E-stained (C,E) and PAS-stained (D,F) sections of the ovaries of young (4 mpf) and old (18 mpf) female zebrafish. Atretic follicles (indicated by arrows) were mostly found in old female fish with low to absent E2 levels. mpf, months post fertilization.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582103&req=5

DMM019950F1: Definition of reproductive age in zebrafish. (A) Serum estradiol levels in female and male zebrafish during ageing. Data are reported as mean±s.d. Final sample number=12 per sex/age. (B) Application of the ovary maturation classification in the evaluation of the effects of ageing on zebrafish ovarian tissue (see supplementary material Fig. S1 for details). Three sections from five fish per subgroup were analyzed. Data were expressed as mean±s.d. H&E-stained (C,E) and PAS-stained (D,F) sections of the ovaries of young (4 mpf) and old (18 mpf) female zebrafish. Atretic follicles (indicated by arrows) were mostly found in old female fish with low to absent E2 levels. mpf, months post fertilization.
Mentions: In order to study the effect of diet at different reproductive ages, we used young and old zebrafish. To substantiate the reproductive age, in particular the ovarian senescence in old female fish, we analyzed the circulating levels of estradiol and performed histopathologic evaluation of ovaries (Fig. 1).Fig. 1.

Bottom Line: In the entire cohort, at multivariate logistic regression, male gender [odds ratio (OR): 1.408, 95% confidence interval (95% CI): 0.779-2.542, P=0.25] vs women at reproductive age was not associated with F2-F4 fibrosis, whereas a trend was observed for menopause (OR: 1.752, 95% CI: 0.956-3.208, P=0.06).In women, menopause (OR: 2.717, 95% CI: 1.020-7.237, P=0.04) was independently associated with F2-F4 fibrosis.Similarly, in overfed zebrafish, old female fish with failing ovarian function [as demonstrated by extremely low circulating estradiol levels (1.4±0.1 pg/µl) and prevailing presence of atretic follicles in the ovaries] developed massive steatosis and substantial fibrosis (comparable with that occurring in males), whereas young female fish developed less steatosis and were totally protected from the development of fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Gastroenterology Unit, Department of Internal Medicine, University of Modena and Reggio Emilia, 41124 Modena, Italy.

No MeSH data available.


Related in: MedlinePlus