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Scaffold attachment factor B2 (SAFB2)- mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1.

Jiang S, Katz TA, Garee JP, DeMayo FJ, Lee AV, Oesterreich S - Dis Model Mech (2015)

Bottom Line: Scaffold attachment factors SAFB1 and SAFB2 are multifunctional proteins that share >70% sequence similarity.Further analysis showed a significantly increased testis weight in SAFB2(-/-) mice, which was associated with an increased number of Sertoli cells.Our data suggest that this is at least in part caused by alterations in androgen-receptor function and expression upon deletion of SAFB2.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA Lester and Sue Smith Breast Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

No MeSH data available.


Related in: MedlinePlus

SAFB2 and SAFB1 protein expression levels in mouse tissues. Immunoblot analysis using antibodies against SAFB2, SAFB1 and Lamin A/C (as loading control). KO, knock out.
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DMM019885F3: SAFB2 and SAFB1 protein expression levels in mouse tissues. Immunoblot analysis using antibodies against SAFB2, SAFB1 and Lamin A/C (as loading control). KO, knock out.

Mentions: To narrow down which tissue could have potential, less obvious, phenotypes in the SAFB2−/− mice, we surveyed a large panel of mouse tissues for SAFB2 protein, and compared it to that of SAFB1. Immunoblot (Fig. 3) and immunohistochemistry (IHC) (Fig. 4) analyses were performed, using tissue from knockout animals as controls (Fig. 4A). Immunoblot data showed high expression of both SAFB1 and SAFB2 in the immune system (spleen, thymus) and in hormonally regulated organs (uterus, ovary). Of interest, in some tissues of the male reproductive tract, including the prostate, coagulating gland, epididymus and seminal vesicle, SAFB2 expression was significantly higher than that of SAFB1. In general, protein expression was concordant between immunoblot and IHC analyses, and IHC data revealed a potential weak cytoplasmic location of SAFB2 in some tissues, such as heart and kidney. These results indicate that SAFB2 might play a role in the male reproductive system.Fig. 3.


Scaffold attachment factor B2 (SAFB2)- mice reveal non-redundant functions of SAFB2 compared with its paralog, SAFB1.

Jiang S, Katz TA, Garee JP, DeMayo FJ, Lee AV, Oesterreich S - Dis Model Mech (2015)

SAFB2 and SAFB1 protein expression levels in mouse tissues. Immunoblot analysis using antibodies against SAFB2, SAFB1 and Lamin A/C (as loading control). KO, knock out.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582101&req=5

DMM019885F3: SAFB2 and SAFB1 protein expression levels in mouse tissues. Immunoblot analysis using antibodies against SAFB2, SAFB1 and Lamin A/C (as loading control). KO, knock out.
Mentions: To narrow down which tissue could have potential, less obvious, phenotypes in the SAFB2−/− mice, we surveyed a large panel of mouse tissues for SAFB2 protein, and compared it to that of SAFB1. Immunoblot (Fig. 3) and immunohistochemistry (IHC) (Fig. 4) analyses were performed, using tissue from knockout animals as controls (Fig. 4A). Immunoblot data showed high expression of both SAFB1 and SAFB2 in the immune system (spleen, thymus) and in hormonally regulated organs (uterus, ovary). Of interest, in some tissues of the male reproductive tract, including the prostate, coagulating gland, epididymus and seminal vesicle, SAFB2 expression was significantly higher than that of SAFB1. In general, protein expression was concordant between immunoblot and IHC analyses, and IHC data revealed a potential weak cytoplasmic location of SAFB2 in some tissues, such as heart and kidney. These results indicate that SAFB2 might play a role in the male reproductive system.Fig. 3.

Bottom Line: Scaffold attachment factors SAFB1 and SAFB2 are multifunctional proteins that share >70% sequence similarity.Further analysis showed a significantly increased testis weight in SAFB2(-/-) mice, which was associated with an increased number of Sertoli cells.Our data suggest that this is at least in part caused by alterations in androgen-receptor function and expression upon deletion of SAFB2.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA Lester and Sue Smith Breast Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

No MeSH data available.


Related in: MedlinePlus