Limits...
Acute perioperative-stress-induced increase of atherosclerotic plaque volume and vulnerability to rupture in apolipoprotein-E-deficient mice is amenable to statin treatment and IL-6 inhibition.

Janssen H, Wagner CS, Demmer P, Callies S, Sölter G, Loghmani-khouzani H, Hu N, Schuett H, Tietge UJ, Warnecke G, Larmann J, Theilmeier G - Dis Model Mech (2015)

Bottom Line: SAA levels peaked at 24 h (n=4, P<0.01).Relative VSMC and macrophage content remained unchanged.IL-6-inhibition or atorvastatin, but not blocking of IL-6 trans-signaling, significantly decreased plaque volume and complexity (n=8, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, 30625 Hannover, Germany Department of Anesthesiology, University of Heidelberg, 69120 Heidelberg, Germany.

No MeSH data available.


Related in: MedlinePlus

Plaque volume in atherosclerosis-prone mice increased with perioperative stress. Matched ApoE-deficient mice of mixed gender, 8 weeks of age, were placed on a Western diet for 7 weeks and subjected to blood loss, laparotomy or the combination thereof to exert perioperative stress (double hit). (A) Plaque area was determined on H&E staining every 42 µm throughout the innominate artery and plaque volume was calculated. Only blood loss or the combination of surgery and blood loss caused a significant increase of plaque volume in the innominate artery. Kruskal–Wallis test. BSL, baseline. (B) Representative micrographs of H&E staining of plaques at the branching of the innominate artery. The overall plaque load was rather small, but eccentric plaques in the double-hit group had complex lesions, whereas animals in the surgery or blood-loss groups had lesions resembling foam cell lesions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4582096&req=5

DMM018713F2: Plaque volume in atherosclerosis-prone mice increased with perioperative stress. Matched ApoE-deficient mice of mixed gender, 8 weeks of age, were placed on a Western diet for 7 weeks and subjected to blood loss, laparotomy or the combination thereof to exert perioperative stress (double hit). (A) Plaque area was determined on H&E staining every 42 µm throughout the innominate artery and plaque volume was calculated. Only blood loss or the combination of surgery and blood loss caused a significant increase of plaque volume in the innominate artery. Kruskal–Wallis test. BSL, baseline. (B) Representative micrographs of H&E staining of plaques at the branching of the innominate artery. The overall plaque load was rather small, but eccentric plaques in the double-hit group had complex lesions, whereas animals in the surgery or blood-loss groups had lesions resembling foam cell lesions.

Mentions: Baseline plaque formation was examined in mice sacrificed after 7 weeks of diet prior to any intervention. Sham animals served as controls for spontaneous plaque growth during the 72 h while interventions took place. Sham mice were sacrificed simultaneously to the single- and double-hit groups. Baseline and sham animals showed no difference in plaque volume. The individual components of the double hit, surgery or blood loss, induced measurable plaque that was nearly absent in sham and baseline animals. Plaque volume in the surgery group was not different from sham animals, but differed significantly between blood-loss and sham mice (P<0.05). The double hit induced the most prominent and significant increase in plaque volume compared to the sham group (P<0.01) (Fig. 2A,B).Fig. 2.


Acute perioperative-stress-induced increase of atherosclerotic plaque volume and vulnerability to rupture in apolipoprotein-E-deficient mice is amenable to statin treatment and IL-6 inhibition.

Janssen H, Wagner CS, Demmer P, Callies S, Sölter G, Loghmani-khouzani H, Hu N, Schuett H, Tietge UJ, Warnecke G, Larmann J, Theilmeier G - Dis Model Mech (2015)

Plaque volume in atherosclerosis-prone mice increased with perioperative stress. Matched ApoE-deficient mice of mixed gender, 8 weeks of age, were placed on a Western diet for 7 weeks and subjected to blood loss, laparotomy or the combination thereof to exert perioperative stress (double hit). (A) Plaque area was determined on H&E staining every 42 µm throughout the innominate artery and plaque volume was calculated. Only blood loss or the combination of surgery and blood loss caused a significant increase of plaque volume in the innominate artery. Kruskal–Wallis test. BSL, baseline. (B) Representative micrographs of H&E staining of plaques at the branching of the innominate artery. The overall plaque load was rather small, but eccentric plaques in the double-hit group had complex lesions, whereas animals in the surgery or blood-loss groups had lesions resembling foam cell lesions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582096&req=5

DMM018713F2: Plaque volume in atherosclerosis-prone mice increased with perioperative stress. Matched ApoE-deficient mice of mixed gender, 8 weeks of age, were placed on a Western diet for 7 weeks and subjected to blood loss, laparotomy or the combination thereof to exert perioperative stress (double hit). (A) Plaque area was determined on H&E staining every 42 µm throughout the innominate artery and plaque volume was calculated. Only blood loss or the combination of surgery and blood loss caused a significant increase of plaque volume in the innominate artery. Kruskal–Wallis test. BSL, baseline. (B) Representative micrographs of H&E staining of plaques at the branching of the innominate artery. The overall plaque load was rather small, but eccentric plaques in the double-hit group had complex lesions, whereas animals in the surgery or blood-loss groups had lesions resembling foam cell lesions.
Mentions: Baseline plaque formation was examined in mice sacrificed after 7 weeks of diet prior to any intervention. Sham animals served as controls for spontaneous plaque growth during the 72 h while interventions took place. Sham mice were sacrificed simultaneously to the single- and double-hit groups. Baseline and sham animals showed no difference in plaque volume. The individual components of the double hit, surgery or blood loss, induced measurable plaque that was nearly absent in sham and baseline animals. Plaque volume in the surgery group was not different from sham animals, but differed significantly between blood-loss and sham mice (P<0.05). The double hit induced the most prominent and significant increase in plaque volume compared to the sham group (P<0.01) (Fig. 2A,B).Fig. 2.

Bottom Line: SAA levels peaked at 24 h (n=4, P<0.01).Relative VSMC and macrophage content remained unchanged.IL-6-inhibition or atorvastatin, but not blocking of IL-6 trans-signaling, significantly decreased plaque volume and complexity (n=8, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, 30625 Hannover, Germany Department of Anesthesiology, University of Heidelberg, 69120 Heidelberg, Germany.

No MeSH data available.


Related in: MedlinePlus