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Acute perioperative-stress-induced increase of atherosclerotic plaque volume and vulnerability to rupture in apolipoprotein-E-deficient mice is amenable to statin treatment and IL-6 inhibition.

Janssen H, Wagner CS, Demmer P, Callies S, Sölter G, Loghmani-khouzani H, Hu N, Schuett H, Tietge UJ, Warnecke G, Larmann J, Theilmeier G - Dis Model Mech (2015)

Bottom Line: SAA levels peaked at 24 h (n=4, P<0.01).Relative VSMC and macrophage content remained unchanged.IL-6-inhibition or atorvastatin, but not blocking of IL-6 trans-signaling, significantly decreased plaque volume and complexity (n=8, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, 30625 Hannover, Germany Department of Anesthesiology, University of Heidelberg, 69120 Heidelberg, Germany.

No MeSH data available.


Related in: MedlinePlus

Lipoprotein profiles and plasma IL-6 levels in C57BL6/J animals and in ApoE-deficient animals on a Western diet subjected to the combination of surgery and blood loss (double hit). (A) For baseline (BSL) measurements, blood was drawn from the retrobulbar plexus without any intervention and lipoprotein profiles were measured. Measurements for the other groups took place after the procedure. There were no differences in any of the components except for a decrease in VLDL cholesterol levels in the surgery group. Kruskal–Wallis and Dunn's post hoc test, n=5 each, P<0.01. (B) ELISA for IL-6 detected a significant increase in wild-type mice subjected to the double-hit protocol. Separate animals had to be used to avoid interference with the plaque growth protocol due to additional blood draws. The IL-6 peak occurred at 6 h after procedure. ApoE-deficient mice on a Western diet had slightly higher IL-6 levels compared to the wild types and the IL-6 increase was significantly higher compared to the baseline levels as well as compared to the levels of the wild-type mice. One-way ANOVA and t-test, n=4, for each time point, *P<0.05; **P<0.01; §P<0.05; vs BSL.
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DMM018713F1: Lipoprotein profiles and plasma IL-6 levels in C57BL6/J animals and in ApoE-deficient animals on a Western diet subjected to the combination of surgery and blood loss (double hit). (A) For baseline (BSL) measurements, blood was drawn from the retrobulbar plexus without any intervention and lipoprotein profiles were measured. Measurements for the other groups took place after the procedure. There were no differences in any of the components except for a decrease in VLDL cholesterol levels in the surgery group. Kruskal–Wallis and Dunn's post hoc test, n=5 each, P<0.01. (B) ELISA for IL-6 detected a significant increase in wild-type mice subjected to the double-hit protocol. Separate animals had to be used to avoid interference with the plaque growth protocol due to additional blood draws. The IL-6 peak occurred at 6 h after procedure. ApoE-deficient mice on a Western diet had slightly higher IL-6 levels compared to the wild types and the IL-6 increase was significantly higher compared to the baseline levels as well as compared to the levels of the wild-type mice. One-way ANOVA and t-test, n=4, for each time point, *P<0.05; **P<0.01; §P<0.05; vs BSL.

Mentions: We measured plasma lipids in a subgroup of animals to examine the effects of the double hit and its components on lipoprotein profiles. In this context, baseline refers to blood drawn from animals without any procedure on the day of the other groups undergoing the specific interventions. In comparison, sham only underwent general anesthesia, with blood draw taking place 72 h later with the other groups. After 7 weeks on Western diet, baseline animals had similar lipoprotein levels as the sham group. Total cholesterol and HDL remained largely unchanged by any intervention. Surgery alone surprisingly led to a significant decrease of very-low-density lipoprotein (VLDL) cholesterol (P<0.05) (Fig. 1A).Fig. 1.


Acute perioperative-stress-induced increase of atherosclerotic plaque volume and vulnerability to rupture in apolipoprotein-E-deficient mice is amenable to statin treatment and IL-6 inhibition.

Janssen H, Wagner CS, Demmer P, Callies S, Sölter G, Loghmani-khouzani H, Hu N, Schuett H, Tietge UJ, Warnecke G, Larmann J, Theilmeier G - Dis Model Mech (2015)

Lipoprotein profiles and plasma IL-6 levels in C57BL6/J animals and in ApoE-deficient animals on a Western diet subjected to the combination of surgery and blood loss (double hit). (A) For baseline (BSL) measurements, blood was drawn from the retrobulbar plexus without any intervention and lipoprotein profiles were measured. Measurements for the other groups took place after the procedure. There were no differences in any of the components except for a decrease in VLDL cholesterol levels in the surgery group. Kruskal–Wallis and Dunn's post hoc test, n=5 each, P<0.01. (B) ELISA for IL-6 detected a significant increase in wild-type mice subjected to the double-hit protocol. Separate animals had to be used to avoid interference with the plaque growth protocol due to additional blood draws. The IL-6 peak occurred at 6 h after procedure. ApoE-deficient mice on a Western diet had slightly higher IL-6 levels compared to the wild types and the IL-6 increase was significantly higher compared to the baseline levels as well as compared to the levels of the wild-type mice. One-way ANOVA and t-test, n=4, for each time point, *P<0.05; **P<0.01; §P<0.05; vs BSL.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582096&req=5

DMM018713F1: Lipoprotein profiles and plasma IL-6 levels in C57BL6/J animals and in ApoE-deficient animals on a Western diet subjected to the combination of surgery and blood loss (double hit). (A) For baseline (BSL) measurements, blood was drawn from the retrobulbar plexus without any intervention and lipoprotein profiles were measured. Measurements for the other groups took place after the procedure. There were no differences in any of the components except for a decrease in VLDL cholesterol levels in the surgery group. Kruskal–Wallis and Dunn's post hoc test, n=5 each, P<0.01. (B) ELISA for IL-6 detected a significant increase in wild-type mice subjected to the double-hit protocol. Separate animals had to be used to avoid interference with the plaque growth protocol due to additional blood draws. The IL-6 peak occurred at 6 h after procedure. ApoE-deficient mice on a Western diet had slightly higher IL-6 levels compared to the wild types and the IL-6 increase was significantly higher compared to the baseline levels as well as compared to the levels of the wild-type mice. One-way ANOVA and t-test, n=4, for each time point, *P<0.05; **P<0.01; §P<0.05; vs BSL.
Mentions: We measured plasma lipids in a subgroup of animals to examine the effects of the double hit and its components on lipoprotein profiles. In this context, baseline refers to blood drawn from animals without any procedure on the day of the other groups undergoing the specific interventions. In comparison, sham only underwent general anesthesia, with blood draw taking place 72 h later with the other groups. After 7 weeks on Western diet, baseline animals had similar lipoprotein levels as the sham group. Total cholesterol and HDL remained largely unchanged by any intervention. Surgery alone surprisingly led to a significant decrease of very-low-density lipoprotein (VLDL) cholesterol (P<0.05) (Fig. 1A).Fig. 1.

Bottom Line: SAA levels peaked at 24 h (n=4, P<0.01).Relative VSMC and macrophage content remained unchanged.IL-6-inhibition or atorvastatin, but not blocking of IL-6 trans-signaling, significantly decreased plaque volume and complexity (n=8, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, 30625 Hannover, Germany Department of Anesthesiology, University of Heidelberg, 69120 Heidelberg, Germany.

No MeSH data available.


Related in: MedlinePlus