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Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.

Song KH, Youn CS, Lee CL, Yang SW, Chang YS, Jeong SW, Sul CK - Int Neurourol J (2015)

Bottom Line: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8).After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).

Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.

Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).

Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

No MeSH data available.


Related in: MedlinePlus

Representative immunoblots (A) and relative distribution of ErbB2 (B) and NRG1 (C) protein in interstitial cystitis (IC) and control groups. β-actin was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to β-actin expression. The asterisk indicates a significant difference (Mann-Whitney test; P<0.05). ErbB2, erbB2 tyrosine kinase; NRG, neuregulin.
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f2-inj-19-3-158: Representative immunoblots (A) and relative distribution of ErbB2 (B) and NRG1 (C) protein in interstitial cystitis (IC) and control groups. β-actin was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to β-actin expression. The asterisk indicates a significant difference (Mann-Whitney test; P<0.05). ErbB2, erbB2 tyrosine kinase; NRG, neuregulin.

Mentions: We examined the expression of NRG1 (44 kDa) and ErbB2 (185 kDa) by immunoblotting analysis in bladder tissues of CYP-induced IC and control rats (Fig. 2A). The relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (1.42±0.09 vs. 0.93±0.15 and 0.93 ±0.16 vs. 0.29±0.08, P<0.05) (Fig. 2B, C).


Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.

Song KH, Youn CS, Lee CL, Yang SW, Chang YS, Jeong SW, Sul CK - Int Neurourol J (2015)

Representative immunoblots (A) and relative distribution of ErbB2 (B) and NRG1 (C) protein in interstitial cystitis (IC) and control groups. β-actin was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to β-actin expression. The asterisk indicates a significant difference (Mann-Whitney test; P<0.05). ErbB2, erbB2 tyrosine kinase; NRG, neuregulin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582087&req=5

f2-inj-19-3-158: Representative immunoblots (A) and relative distribution of ErbB2 (B) and NRG1 (C) protein in interstitial cystitis (IC) and control groups. β-actin was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to β-actin expression. The asterisk indicates a significant difference (Mann-Whitney test; P<0.05). ErbB2, erbB2 tyrosine kinase; NRG, neuregulin.
Mentions: We examined the expression of NRG1 (44 kDa) and ErbB2 (185 kDa) by immunoblotting analysis in bladder tissues of CYP-induced IC and control rats (Fig. 2A). The relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (1.42±0.09 vs. 0.93±0.15 and 0.93 ±0.16 vs. 0.29±0.08, P<0.05) (Fig. 2B, C).

Bottom Line: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8).After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).

Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.

Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).

Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

No MeSH data available.


Related in: MedlinePlus