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Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.

Song KH, Youn CS, Lee CL, Yang SW, Chang YS, Jeong SW, Sul CK - Int Neurourol J (2015)

Bottom Line: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8).After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).

Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.

Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).

Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

No MeSH data available.


Related in: MedlinePlus

Representative mRNA expression and relative distribution of NRG1 (A) and ErbB2 (B) in interstitial cystitis (IC) and control groups. GAPDH was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to GAPDH expression. The asterisk indicates a significant difference (P<0.05). NRG, neuregulin; ErbB2, erbB2 tyrosine kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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f1-inj-19-3-158: Representative mRNA expression and relative distribution of NRG1 (A) and ErbB2 (B) in interstitial cystitis (IC) and control groups. GAPDH was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to GAPDH expression. The asterisk indicates a significant difference (P<0.05). NRG, neuregulin; ErbB2, erbB2 tyrosine kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Mentions: We examined the mRNA expression levels of NRG1 and ErbB2 in bladder tissues from the CYP-induced cystitis and control groups using RT-PCR. The mRNA expression level of NRG1 was higher in bladder samples of rats with CYP-induced IC than in those of control rats (Fig. 1A) (P<0.05). The mRNA expression of ErbB2 yielded similar results to that of NRG1. As a whole, CYP-induced IC showed significantly higher levels of ErbB2 (Fig. 1B) (P<0.05). Gene expression was normalized to the expression of GAPDH.


Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.

Song KH, Youn CS, Lee CL, Yang SW, Chang YS, Jeong SW, Sul CK - Int Neurourol J (2015)

Representative mRNA expression and relative distribution of NRG1 (A) and ErbB2 (B) in interstitial cystitis (IC) and control groups. GAPDH was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to GAPDH expression. The asterisk indicates a significant difference (P<0.05). NRG, neuregulin; ErbB2, erbB2 tyrosine kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582087&req=5

f1-inj-19-3-158: Representative mRNA expression and relative distribution of NRG1 (A) and ErbB2 (B) in interstitial cystitis (IC) and control groups. GAPDH was used as the loading control. Values of NRG1 and ErbB2 expression are normalized relative to GAPDH expression. The asterisk indicates a significant difference (P<0.05). NRG, neuregulin; ErbB2, erbB2 tyrosine kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mentions: We examined the mRNA expression levels of NRG1 and ErbB2 in bladder tissues from the CYP-induced cystitis and control groups using RT-PCR. The mRNA expression level of NRG1 was higher in bladder samples of rats with CYP-induced IC than in those of control rats (Fig. 1A) (P<0.05). The mRNA expression of ErbB2 yielded similar results to that of NRG1. As a whole, CYP-induced IC showed significantly higher levels of ErbB2 (Fig. 1B) (P<0.05). Gene expression was normalized to the expression of GAPDH.

Bottom Line: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8).After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).

Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.

Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).

Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

No MeSH data available.


Related in: MedlinePlus