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Inhibitory Effect and Possible Mechanism of Intraurethral Stimulation on Overactive Bladder in Female Rats.

Tian Y, Liao L, Wyndaele JJ - Int Neurourol J (2015)

Bottom Line: Cystometry was performed in 13 urethane-anesthetized female rats.Infusion of acetic acid significantly decreased bladder capacity.Activation of urethral afferent nerves can reverse OAB, which activates C-fiber afferent nerves.

View Article: PubMed Central - PubMed

Affiliation: Department Translational Neurosciences, Laboratory Urology, University of Antwerp, Faculty GGW, Antwerp, Belgium ; Department of Urology, University Hospital Antwerp, Antwerp, Belgium ; Department of Urology, China Rehabilitation Research Center, Capital Medical University, Beijing, China.

ABSTRACT

Purpose: To investigate the inhibitory effect and possible mechanism of intraurethral stimulation on overactive bladder (OAB) induced by acetic acid irritation.

Methods: Cystometry was performed in 13 urethane-anesthetized female rats. Intravesical infusion of 0.5% acetic acid was used to irritate the bladder and induce OAB. Multiple cystometrograms were performed with mirabegron, continuous stimulation, mirabegron plus continuous stimulation, and β3-adrenoceptor antagonist plus continuous stimulation to determine the mechanism underlying the inhibitory effect by intraurethral stimulation.

Results: Infusion of acetic acid significantly decreased bladder capacity. Intraurethral stimulation at 2.5 Hz plus mirabegron significantly increased bladder capacity and decreased the nonvoiding contraction count. The changes were strongly inhibited after the β3-adrenoceptor antagonist was administered.

Conclusions: Activation of urethral afferent nerves can reverse OAB, which activates C-fiber afferent nerves. This animal study indicates that intraurethral stimulation may interfere with OAB through hypogastric nerve activation and pudendal nerve neuromodulation.

No MeSH data available.


Related in: MedlinePlus

Electrode setup and location. (A) Two coated platinum wires were 9 and 7 mm longer than the catheter. (B) The electrodes were fully inserted into the catheter. The catheter and the wires were moved outward together until the catheter was nearly out of the urethra. (C) The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the two electrodes in place.
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f1-inj-19-3-151: Electrode setup and location. (A) Two coated platinum wires were 9 and 7 mm longer than the catheter. (B) The electrodes were fully inserted into the catheter. The catheter and the wires were moved outward together until the catheter was nearly out of the urethra. (C) The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the two electrodes in place.

Mentions: A 20-gauge intravenous catheter (Becton-Dickinson, Madrid, Spain) was inserted into the urethra via the urethral meatus. Two coated platinum wires, which were 9 and 7 mm longer than the catheter (Fig. 1A), were fully inserted into the catheter, and then the catheter together with the wires was moved outward until the catheter was nearly out of the urethra. The wires were now positioned in the urethra 9 and 7 mm from the meatus, thus permitting bipolar stimulation in the urethral lumen (Fig. 1B). The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the electrodes in place (Fig. 1C).


Inhibitory Effect and Possible Mechanism of Intraurethral Stimulation on Overactive Bladder in Female Rats.

Tian Y, Liao L, Wyndaele JJ - Int Neurourol J (2015)

Electrode setup and location. (A) Two coated platinum wires were 9 and 7 mm longer than the catheter. (B) The electrodes were fully inserted into the catheter. The catheter and the wires were moved outward together until the catheter was nearly out of the urethra. (C) The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the two electrodes in place.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582086&req=5

f1-inj-19-3-151: Electrode setup and location. (A) Two coated platinum wires were 9 and 7 mm longer than the catheter. (B) The electrodes were fully inserted into the catheter. The catheter and the wires were moved outward together until the catheter was nearly out of the urethra. (C) The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the two electrodes in place.
Mentions: A 20-gauge intravenous catheter (Becton-Dickinson, Madrid, Spain) was inserted into the urethra via the urethral meatus. Two coated platinum wires, which were 9 and 7 mm longer than the catheter (Fig. 1A), were fully inserted into the catheter, and then the catheter together with the wires was moved outward until the catheter was nearly out of the urethra. The wires were now positioned in the urethra 9 and 7 mm from the meatus, thus permitting bipolar stimulation in the urethral lumen (Fig. 1B). The urethral meatus was tied around the wires with 3-0 silk to prevent leakage and hold the electrodes in place (Fig. 1C).

Bottom Line: Cystometry was performed in 13 urethane-anesthetized female rats.Infusion of acetic acid significantly decreased bladder capacity.Activation of urethral afferent nerves can reverse OAB, which activates C-fiber afferent nerves.

View Article: PubMed Central - PubMed

Affiliation: Department Translational Neurosciences, Laboratory Urology, University of Antwerp, Faculty GGW, Antwerp, Belgium ; Department of Urology, University Hospital Antwerp, Antwerp, Belgium ; Department of Urology, China Rehabilitation Research Center, Capital Medical University, Beijing, China.

ABSTRACT

Purpose: To investigate the inhibitory effect and possible mechanism of intraurethral stimulation on overactive bladder (OAB) induced by acetic acid irritation.

Methods: Cystometry was performed in 13 urethane-anesthetized female rats. Intravesical infusion of 0.5% acetic acid was used to irritate the bladder and induce OAB. Multiple cystometrograms were performed with mirabegron, continuous stimulation, mirabegron plus continuous stimulation, and β3-adrenoceptor antagonist plus continuous stimulation to determine the mechanism underlying the inhibitory effect by intraurethral stimulation.

Results: Infusion of acetic acid significantly decreased bladder capacity. Intraurethral stimulation at 2.5 Hz plus mirabegron significantly increased bladder capacity and decreased the nonvoiding contraction count. The changes were strongly inhibited after the β3-adrenoceptor antagonist was administered.

Conclusions: Activation of urethral afferent nerves can reverse OAB, which activates C-fiber afferent nerves. This animal study indicates that intraurethral stimulation may interfere with OAB through hypogastric nerve activation and pudendal nerve neuromodulation.

No MeSH data available.


Related in: MedlinePlus