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Patterns of binding of aluminum-containing adjuvants to Haemophilus influenzae type b and meningococcal group C conjugate vaccines and components.

Otto RB, Burkin K, Amir SE, Crane DT, Bolgiano B - Biologicals (2015)

Bottom Line: By measuring the amount of polysaccharide and protein in the non-adsorbed supernatant, the adjuvant, aluminum phosphate, AlPO4, was found to be less efficient than aluminum hydroxide, Al(OH)3 at binding to the conjugates, at concentrations relevant to licensed vaccine formulations and when equimolar.At neutral pH, the anionic Hib and MenC polysaccharides did not appreciably bind to AlPO4, but did bind to Al(OH)3, due to electrostatic interactions.Phosphate ions reduced the binding of the conjugates to the adjuvants.

View Article: PubMed Central - PubMed

Affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, United Kingdom. Electronic address: r.bdotto@yahoo.com.

No MeSH data available.


Related in: MedlinePlus

Adsorption of Hib conjugate in DTwP-combination vaccines. The adsorption of Hib conjugates to aluminum phosphate in DTwP combination vaccines was measured in TT conjugates (C and D series), and CRM197 conjugates (E and F series). The numeral in the code represents different manufacturing lots of the same vaccine. The % adsorbed values were determined from measure of PRP remaining in the supernatant of spun-desorbed samples relative to that in untreated vaccine. The error bars represent the combined standard uncertainty of the assay.
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fig2: Adsorption of Hib conjugate in DTwP-combination vaccines. The adsorption of Hib conjugates to aluminum phosphate in DTwP combination vaccines was measured in TT conjugates (C and D series), and CRM197 conjugates (E and F series). The numeral in the code represents different manufacturing lots of the same vaccine. The % adsorbed values were determined from measure of PRP remaining in the supernatant of spun-desorbed samples relative to that in untreated vaccine. The error bars represent the combined standard uncertainty of the assay.

Mentions: The adsorption of the Hib component of DTwP combination vaccines was evaluated for two CRM197 and TT-based conjugates in aluminum phosphate formulations, pH 6.0–6.5. There was considerable adsorption of the TT-conjugates to adjuvant (25–65%), with vaccine D being relatively more adsorbent than vaccine C (Fig. 2). Saydam et al. also found variable adsorption of similar vaccines by ELISA [51]. Only slight adsorption (up to 10%) of the CRM197-conjugates was found.


Patterns of binding of aluminum-containing adjuvants to Haemophilus influenzae type b and meningococcal group C conjugate vaccines and components.

Otto RB, Burkin K, Amir SE, Crane DT, Bolgiano B - Biologicals (2015)

Adsorption of Hib conjugate in DTwP-combination vaccines. The adsorption of Hib conjugates to aluminum phosphate in DTwP combination vaccines was measured in TT conjugates (C and D series), and CRM197 conjugates (E and F series). The numeral in the code represents different manufacturing lots of the same vaccine. The % adsorbed values were determined from measure of PRP remaining in the supernatant of spun-desorbed samples relative to that in untreated vaccine. The error bars represent the combined standard uncertainty of the assay.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582044&req=5

fig2: Adsorption of Hib conjugate in DTwP-combination vaccines. The adsorption of Hib conjugates to aluminum phosphate in DTwP combination vaccines was measured in TT conjugates (C and D series), and CRM197 conjugates (E and F series). The numeral in the code represents different manufacturing lots of the same vaccine. The % adsorbed values were determined from measure of PRP remaining in the supernatant of spun-desorbed samples relative to that in untreated vaccine. The error bars represent the combined standard uncertainty of the assay.
Mentions: The adsorption of the Hib component of DTwP combination vaccines was evaluated for two CRM197 and TT-based conjugates in aluminum phosphate formulations, pH 6.0–6.5. There was considerable adsorption of the TT-conjugates to adjuvant (25–65%), with vaccine D being relatively more adsorbent than vaccine C (Fig. 2). Saydam et al. also found variable adsorption of similar vaccines by ELISA [51]. Only slight adsorption (up to 10%) of the CRM197-conjugates was found.

Bottom Line: By measuring the amount of polysaccharide and protein in the non-adsorbed supernatant, the adjuvant, aluminum phosphate, AlPO4, was found to be less efficient than aluminum hydroxide, Al(OH)3 at binding to the conjugates, at concentrations relevant to licensed vaccine formulations and when equimolar.At neutral pH, the anionic Hib and MenC polysaccharides did not appreciably bind to AlPO4, but did bind to Al(OH)3, due to electrostatic interactions.Phosphate ions reduced the binding of the conjugates to the adjuvants.

View Article: PubMed Central - PubMed

Affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, United Kingdom. Electronic address: r.bdotto@yahoo.com.

No MeSH data available.


Related in: MedlinePlus