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Amino acids in sandal (Santalum album L) with special reference to cis-4-hydroxy-l-proline and sym. homospermidine.

Kuttan R, Panikkar B, Binitha PP - Springerplus (2015)

Bottom Line: Sandal also contains sym. homospermidine which is not present in any other plants till today.Moreover sandal leaves contain γ-glutamyl derivative of the lachrymatory precursor of onion, γ-glutamyl-S-propenyl cysteine superoxide.This review summarizes the studies on the amino acids in sandal.

View Article: PubMed Central - PubMed

Affiliation: Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala 680555 India.

ABSTRACT
Sandal (Santalum album L) contains several interesting amino acids and amines which are not seen in other plants. This includes cis-4-hydroxy-l-proline in free form in leaves, flowers and seeds while trans-4-hydroxy-l-proline in bound form. Traces of 3, 4 dehydroproline is also detected in sandal leaves. Biosynthesis of cis-4-hydroxy proline indicates that hydroxylation taken place at proline present in peptidyl form especially bound to glutamic acid and aspartic acid. Pyrrolizidine-2-carboxylic acid an interesting isatin positive heterocyclic compound is also present in sandal leaves. Sandal also contains sym. homospermidine which is not present in any other plants till today. Biosynthesis of sym. homospermidine goes by a unique pathway of putrescine oxidation, Schiff base formation, condensation and reduction. Moreover sandal leaves contain γ-glutamyl derivative of the lachrymatory precursor of onion, γ-glutamyl-S-propenyl cysteine superoxide. This review summarizes the studies on the amino acids in sandal.

No MeSH data available.


Related in: MedlinePlus

Two dimensional paper chromatography of Ehrlich positive material. 1cis-4-hydroxy proline. 2 Proline. 3, 4, 5 Ehrlich positive material
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Fig3: Two dimensional paper chromatography of Ehrlich positive material. 1cis-4-hydroxy proline. 2 Proline. 3, 4, 5 Ehrlich positive material

Mentions: Earlier work by Radhakrishnan indicated that the sandal leaf extract contained a material that reacted with Ehrlich reagent (unpublished). This material remained in the origin during paper chromatography using butanol-acetic acid–water as solvent systems. When 0.75 N HCl elutes of sandal extracts were analysed by paper chromatography there was documented evidence of at least two Ehrlich positive materials which appeared below hydroxyl proline. Large scale isolation of Ehrlich positive material was attempted using Dowex column chromatography and further purification of this material using paper chromatography. The purified material reacted faintly with ninhydrin. Two dimensional paper chromatography of the Ehrlich positive material indicated mobility similar to hydroxyl proline in phenol run and below hydroxyl proline in butanol run (Fig. 3). The Rf values of Ehrlich positive material in butanol-acetic acid–water (4:1:1) system was 0.25 and in phenol–KCl:HCl system was 0.57. The partially purified material was found to be highly unstable and it was converted to hydroxyl proline. Stereo configuration of hydroxy proline produced by hydrolysis was found to be cis-4-hydroxy-l-proline using amino acid analyser in which cis and trans hydroxyproline separated.Fig. 3


Amino acids in sandal (Santalum album L) with special reference to cis-4-hydroxy-l-proline and sym. homospermidine.

Kuttan R, Panikkar B, Binitha PP - Springerplus (2015)

Two dimensional paper chromatography of Ehrlich positive material. 1cis-4-hydroxy proline. 2 Proline. 3, 4, 5 Ehrlich positive material
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582034&req=5

Fig3: Two dimensional paper chromatography of Ehrlich positive material. 1cis-4-hydroxy proline. 2 Proline. 3, 4, 5 Ehrlich positive material
Mentions: Earlier work by Radhakrishnan indicated that the sandal leaf extract contained a material that reacted with Ehrlich reagent (unpublished). This material remained in the origin during paper chromatography using butanol-acetic acid–water as solvent systems. When 0.75 N HCl elutes of sandal extracts were analysed by paper chromatography there was documented evidence of at least two Ehrlich positive materials which appeared below hydroxyl proline. Large scale isolation of Ehrlich positive material was attempted using Dowex column chromatography and further purification of this material using paper chromatography. The purified material reacted faintly with ninhydrin. Two dimensional paper chromatography of the Ehrlich positive material indicated mobility similar to hydroxyl proline in phenol run and below hydroxyl proline in butanol run (Fig. 3). The Rf values of Ehrlich positive material in butanol-acetic acid–water (4:1:1) system was 0.25 and in phenol–KCl:HCl system was 0.57. The partially purified material was found to be highly unstable and it was converted to hydroxyl proline. Stereo configuration of hydroxy proline produced by hydrolysis was found to be cis-4-hydroxy-l-proline using amino acid analyser in which cis and trans hydroxyproline separated.Fig. 3

Bottom Line: Sandal also contains sym. homospermidine which is not present in any other plants till today.Moreover sandal leaves contain γ-glutamyl derivative of the lachrymatory precursor of onion, γ-glutamyl-S-propenyl cysteine superoxide.This review summarizes the studies on the amino acids in sandal.

View Article: PubMed Central - PubMed

Affiliation: Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala 680555 India.

ABSTRACT
Sandal (Santalum album L) contains several interesting amino acids and amines which are not seen in other plants. This includes cis-4-hydroxy-l-proline in free form in leaves, flowers and seeds while trans-4-hydroxy-l-proline in bound form. Traces of 3, 4 dehydroproline is also detected in sandal leaves. Biosynthesis of cis-4-hydroxy proline indicates that hydroxylation taken place at proline present in peptidyl form especially bound to glutamic acid and aspartic acid. Pyrrolizidine-2-carboxylic acid an interesting isatin positive heterocyclic compound is also present in sandal leaves. Sandal also contains sym. homospermidine which is not present in any other plants till today. Biosynthesis of sym. homospermidine goes by a unique pathway of putrescine oxidation, Schiff base formation, condensation and reduction. Moreover sandal leaves contain γ-glutamyl derivative of the lachrymatory precursor of onion, γ-glutamyl-S-propenyl cysteine superoxide. This review summarizes the studies on the amino acids in sandal.

No MeSH data available.


Related in: MedlinePlus