Limits...
Gonadotropin‑releasing hormone inhibits the proliferation and motility of nasopharyngeal carcinoma cells.

Teng LH, Ahmad M, Ng WT, Sabaratnam S, Rasan MI, Parhar I, Khoo AS - Mol Med Rep (2015)

Bottom Line: GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study.Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells.In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur 50588, Malaysia.

ABSTRACT
Gonadotropin‑releasing hormone (GnRH), or its analogues have been demonstrated to exhibit anti‑proliferative effects on tumour cells in ovarian, endometrial and breast cancer through GnRH‑receptors (GnRH‑R). However, the role of GnRH in nasopharyngeal carcinoma (NPC) remains to be elucidated. In order to investigate the effects of GnRH in NPC, the present study examined the expression of the GnRH‑R transcript in NPC and investigated the phenotypic changes in HK1 cells, a recurrent NPC‑derived cell line, upon receiving GnRH treatment. Firstly, the GnRH‑R transcript was demonstrated in the NPC cell lines and four snap frozen biopsies using reverse transcription‑quantitative polymerase chain reaction. In addition, immunohistochemistry revealed the expression of GnRH‑R in two of the eight (25%) NPC specimens. Treatment with GnRH induced a rapid increase in intracellular ionised calcium concentration in the NPC cells. GnRH and its agonists, triptorelin and leuprolide, exerted anti‑proliferative effects on the NPC cells, as determined using an MTS assay. GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study. Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells. In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells. The consequences of alterations in the levels of GnRH on the progression of NPC require further examination.

Show MeSH

Related in: MedlinePlus

GnRH reduces the motility of HK1 cells. (A) Images of the cells were captured under a time-lapse microscope at 10 min intervals for 16 h following treatment with GnRH. The GnRH-treated cells exhibited reduced motility, compared with the control. Blebbing and rupture of cell membranes were observed following 8 h of GnRH treatment. (B) Maximum speed of each cell was measured and the mean was compared between GnRH-treated and control cells. GnRH reduced cell motility (*P<0.05). Experiments were repeated three times, with representative data presented as the mean ± standard deviation. GnRH, gonadotropin releasing hormone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581822&req=5

f5-mmr-12-04-4909: GnRH reduces the motility of HK1 cells. (A) Images of the cells were captured under a time-lapse microscope at 10 min intervals for 16 h following treatment with GnRH. The GnRH-treated cells exhibited reduced motility, compared with the control. Blebbing and rupture of cell membranes were observed following 8 h of GnRH treatment. (B) Maximum speed of each cell was measured and the mean was compared between GnRH-treated and control cells. GnRH reduced cell motility (*P<0.05). Experiments were repeated three times, with representative data presented as the mean ± standard deviation. GnRH, gonadotropin releasing hormone.

Mentions: The HK1 cells were treated with 10−10 M GnRH in low serum medium prior to time-lapse-live cell microscopic imaging. The morphology and migration of the HK1 cells were observed for 16 h with an interval of 10 min. The control (untreated) cells were found to proliferate, while the treated cells were found to undergo apoptosis (Fig. 5A). In general, cell motility was decreased by GnRH, which was four times slower (Fig. 5B), compared with the untreated cells.


Gonadotropin‑releasing hormone inhibits the proliferation and motility of nasopharyngeal carcinoma cells.

Teng LH, Ahmad M, Ng WT, Sabaratnam S, Rasan MI, Parhar I, Khoo AS - Mol Med Rep (2015)

GnRH reduces the motility of HK1 cells. (A) Images of the cells were captured under a time-lapse microscope at 10 min intervals for 16 h following treatment with GnRH. The GnRH-treated cells exhibited reduced motility, compared with the control. Blebbing and rupture of cell membranes were observed following 8 h of GnRH treatment. (B) Maximum speed of each cell was measured and the mean was compared between GnRH-treated and control cells. GnRH reduced cell motility (*P<0.05). Experiments were repeated three times, with representative data presented as the mean ± standard deviation. GnRH, gonadotropin releasing hormone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581822&req=5

f5-mmr-12-04-4909: GnRH reduces the motility of HK1 cells. (A) Images of the cells were captured under a time-lapse microscope at 10 min intervals for 16 h following treatment with GnRH. The GnRH-treated cells exhibited reduced motility, compared with the control. Blebbing and rupture of cell membranes were observed following 8 h of GnRH treatment. (B) Maximum speed of each cell was measured and the mean was compared between GnRH-treated and control cells. GnRH reduced cell motility (*P<0.05). Experiments were repeated three times, with representative data presented as the mean ± standard deviation. GnRH, gonadotropin releasing hormone.
Mentions: The HK1 cells were treated with 10−10 M GnRH in low serum medium prior to time-lapse-live cell microscopic imaging. The morphology and migration of the HK1 cells were observed for 16 h with an interval of 10 min. The control (untreated) cells were found to proliferate, while the treated cells were found to undergo apoptosis (Fig. 5A). In general, cell motility was decreased by GnRH, which was four times slower (Fig. 5B), compared with the untreated cells.

Bottom Line: GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study.Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells.In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur 50588, Malaysia.

ABSTRACT
Gonadotropin‑releasing hormone (GnRH), or its analogues have been demonstrated to exhibit anti‑proliferative effects on tumour cells in ovarian, endometrial and breast cancer through GnRH‑receptors (GnRH‑R). However, the role of GnRH in nasopharyngeal carcinoma (NPC) remains to be elucidated. In order to investigate the effects of GnRH in NPC, the present study examined the expression of the GnRH‑R transcript in NPC and investigated the phenotypic changes in HK1 cells, a recurrent NPC‑derived cell line, upon receiving GnRH treatment. Firstly, the GnRH‑R transcript was demonstrated in the NPC cell lines and four snap frozen biopsies using reverse transcription‑quantitative polymerase chain reaction. In addition, immunohistochemistry revealed the expression of GnRH‑R in two of the eight (25%) NPC specimens. Treatment with GnRH induced a rapid increase in intracellular ionised calcium concentration in the NPC cells. GnRH and its agonists, triptorelin and leuprolide, exerted anti‑proliferative effects on the NPC cells, as determined using an MTS assay. GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study. Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells. In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells. The consequences of alterations in the levels of GnRH on the progression of NPC require further examination.

Show MeSH
Related in: MedlinePlus