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Gonadotropin‑releasing hormone inhibits the proliferation and motility of nasopharyngeal carcinoma cells.

Teng LH, Ahmad M, Ng WT, Sabaratnam S, Rasan MI, Parhar I, Khoo AS - Mol Med Rep (2015)

Bottom Line: GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study.Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells.In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur 50588, Malaysia.

ABSTRACT
Gonadotropin‑releasing hormone (GnRH), or its analogues have been demonstrated to exhibit anti‑proliferative effects on tumour cells in ovarian, endometrial and breast cancer through GnRH‑receptors (GnRH‑R). However, the role of GnRH in nasopharyngeal carcinoma (NPC) remains to be elucidated. In order to investigate the effects of GnRH in NPC, the present study examined the expression of the GnRH‑R transcript in NPC and investigated the phenotypic changes in HK1 cells, a recurrent NPC‑derived cell line, upon receiving GnRH treatment. Firstly, the GnRH‑R transcript was demonstrated in the NPC cell lines and four snap frozen biopsies using reverse transcription‑quantitative polymerase chain reaction. In addition, immunohistochemistry revealed the expression of GnRH‑R in two of the eight (25%) NPC specimens. Treatment with GnRH induced a rapid increase in intracellular ionised calcium concentration in the NPC cells. GnRH and its agonists, triptorelin and leuprolide, exerted anti‑proliferative effects on the NPC cells, as determined using an MTS assay. GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study. Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells. In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells. The consequences of alterations in the levels of GnRH on the progression of NPC require further examination.

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Related in: MedlinePlus

Cell cycle analysis of HK1 cells treated with GnRH. DNA content was measured using flow cytometry following treatment of the cells with GnRH for 48 h. No significant difference was observed (P>0.05) in the cell cycle phases between the untreated and GnRH-treated cells under the conditions assessed. Experiments were repeated three times, with representative data presented. GnRH, gonadotropin releasing hormone.
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f4-mmr-12-04-4909: Cell cycle analysis of HK1 cells treated with GnRH. DNA content was measured using flow cytometry following treatment of the cells with GnRH for 48 h. No significant difference was observed (P>0.05) in the cell cycle phases between the untreated and GnRH-treated cells under the conditions assessed. Experiments were repeated three times, with representative data presented. GnRH, gonadotropin releasing hormone.

Mentions: DNA contents of GnRH treated or control HK1 cells were quantitated for cell cycle analysis. The distribution of cells in the different phases of the cell cycle did not significantly change following GnRH exposure (Fig. 4), suggesting that GnRH did not cause cell cycle arrest under the conditions described.


Gonadotropin‑releasing hormone inhibits the proliferation and motility of nasopharyngeal carcinoma cells.

Teng LH, Ahmad M, Ng WT, Sabaratnam S, Rasan MI, Parhar I, Khoo AS - Mol Med Rep (2015)

Cell cycle analysis of HK1 cells treated with GnRH. DNA content was measured using flow cytometry following treatment of the cells with GnRH for 48 h. No significant difference was observed (P>0.05) in the cell cycle phases between the untreated and GnRH-treated cells under the conditions assessed. Experiments were repeated three times, with representative data presented. GnRH, gonadotropin releasing hormone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581822&req=5

f4-mmr-12-04-4909: Cell cycle analysis of HK1 cells treated with GnRH. DNA content was measured using flow cytometry following treatment of the cells with GnRH for 48 h. No significant difference was observed (P>0.05) in the cell cycle phases between the untreated and GnRH-treated cells under the conditions assessed. Experiments were repeated three times, with representative data presented. GnRH, gonadotropin releasing hormone.
Mentions: DNA contents of GnRH treated or control HK1 cells were quantitated for cell cycle analysis. The distribution of cells in the different phases of the cell cycle did not significantly change following GnRH exposure (Fig. 4), suggesting that GnRH did not cause cell cycle arrest under the conditions described.

Bottom Line: GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study.Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells.In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur 50588, Malaysia.

ABSTRACT
Gonadotropin‑releasing hormone (GnRH), or its analogues have been demonstrated to exhibit anti‑proliferative effects on tumour cells in ovarian, endometrial and breast cancer through GnRH‑receptors (GnRH‑R). However, the role of GnRH in nasopharyngeal carcinoma (NPC) remains to be elucidated. In order to investigate the effects of GnRH in NPC, the present study examined the expression of the GnRH‑R transcript in NPC and investigated the phenotypic changes in HK1 cells, a recurrent NPC‑derived cell line, upon receiving GnRH treatment. Firstly, the GnRH‑R transcript was demonstrated in the NPC cell lines and four snap frozen biopsies using reverse transcription‑quantitative polymerase chain reaction. In addition, immunohistochemistry revealed the expression of GnRH‑R in two of the eight (25%) NPC specimens. Treatment with GnRH induced a rapid increase in intracellular ionised calcium concentration in the NPC cells. GnRH and its agonists, triptorelin and leuprolide, exerted anti‑proliferative effects on the NPC cells, as determined using an MTS assay. GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study. Time‑lapse imaging demonstrated a reduction in cell motility in the GnRH‑treated cells. In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells. The consequences of alterations in the levels of GnRH on the progression of NPC require further examination.

Show MeSH
Related in: MedlinePlus