Limits...
Substrate-specific gene expression profiles in different kidney cell types are associated with Fabry disease.

Shin YJ, Jeon YJ, Jung N, Park JW, Park HY, Jung SC - Mol Med Rep (2015)

Bottom Line: The majority of the biological functions of the regulated genes were associated with fibrogenesis or epithelial‑mesenchymal transition (EMT).In the HK‑2 cells, the ADAMTS6, BEST1, IL4, and MYH11 genes were upregulated.The gene expression profiles in kidney cells following the addition of Gb3 or lyso‑Gb3 revealed substrate‑specific and cell‑specific patterns.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 158‑710, Republic of Korea.

ABSTRACT
Fabry disease is an X-linked lysosomal storage disorder caused by mutations in the gene encoding the α-galactosidase A (α-Gal A) lysosomal enzyme, which results in globotriaosylceramide (Gb3) storage in vascular endothelial cells and different cell types throughout the body. Involvement of the kidney and heart is life threatening, and fibrosis of these organs is considered to be involved in the pathogenesis of Fabry disease. An increased concentration of deacylated Gb3 (lyso‑Gb3) in the plasma of symptomatic patients has also been suggested as a causative molecular event. To elucidate the molecular mechanisms involved in renal fibrosis in Fabry disease, the present analyzed the changes in global gene expression prior to and following Gb3 or lyso‑Gb3 treatment in two types of kidney cell lines, human proximal renal tubular epithelial (HK‑2) and mouse renal glomerular mesangial (SV40 MES 13) cells. Gb3 and lyso‑Gb3 treatment regulated the expression of 199 and 328 genes in each cell type, demonstrating a >2.0‑fold change. The majority of the biological functions of the regulated genes were associated with fibrogenesis or epithelial‑mesenchymal transition (EMT). The gene expression patterns of sphingolipid‑treated HK‑2 cells were distinguishable from the patterns in the SV40 MES 13 cells. Several genes associated with the EMT were selected and evaluated further in kidney cells and in Fabry mouse kidney tissues. In the SV40 MES 13 cells, the DLL1, F8, and HOXA11 genes were downregulated, and FOXP2 was upregulated by treatment with Gb3 or lyso‑Gb3. In the HK‑2 cells, the ADAMTS6, BEST1, IL4, and MYH11 genes were upregulated. Upregulation of the FOXP2, COL15A1, IL4, and MYH11 genes was also observed in the Fabry mouse kidney tissues. The gene expression profiles in kidney cells following the addition of Gb3 or lyso‑Gb3 revealed substrate‑specific and cell‑specific patterns. These findings suggested that Gb3 and lyso‑Gb3 lead to renal fibrosis in Fabry disease through different biochemical modulations.

Show MeSH

Related in: MedlinePlus

Reverse transcription-quantitative polymerase chain reaction analyses of the FOXP2, COL15A1, IL4, and MYH11 genes in mouse kidney tissues. The gene expression ratios of each gene in the kidneys were compared between Fabry mice and wild-type mice. Data are expressed as the mean ± standard error of the mean of at least three experiments. *P<0.05, wild-type, vs. Fabry tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581816&req=5

f4-mmr-12-04-5049: Reverse transcription-quantitative polymerase chain reaction analyses of the FOXP2, COL15A1, IL4, and MYH11 genes in mouse kidney tissues. The gene expression ratios of each gene in the kidneys were compared between Fabry mice and wild-type mice. Data are expressed as the mean ± standard error of the mean of at least three experiments. *P<0.05, wild-type, vs. Fabry tissues.

Mentions: The present study also examined the expression levels of FOXP2, COL15A1, MYH11 and IL4 in kidney tissues from wild-type and Fabry mice (Fig. 4). Compared with the wild-type tissues, the expression levels of COL15A1, IL4 and MYH11 were significantly upregulated in the Fabry mouse kidney tissues. No significant upregulation in the expression of FOXP2 was observed in the Fabry mouse kidney (P=0.059).


Substrate-specific gene expression profiles in different kidney cell types are associated with Fabry disease.

Shin YJ, Jeon YJ, Jung N, Park JW, Park HY, Jung SC - Mol Med Rep (2015)

Reverse transcription-quantitative polymerase chain reaction analyses of the FOXP2, COL15A1, IL4, and MYH11 genes in mouse kidney tissues. The gene expression ratios of each gene in the kidneys were compared between Fabry mice and wild-type mice. Data are expressed as the mean ± standard error of the mean of at least three experiments. *P<0.05, wild-type, vs. Fabry tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581816&req=5

f4-mmr-12-04-5049: Reverse transcription-quantitative polymerase chain reaction analyses of the FOXP2, COL15A1, IL4, and MYH11 genes in mouse kidney tissues. The gene expression ratios of each gene in the kidneys were compared between Fabry mice and wild-type mice. Data are expressed as the mean ± standard error of the mean of at least three experiments. *P<0.05, wild-type, vs. Fabry tissues.
Mentions: The present study also examined the expression levels of FOXP2, COL15A1, MYH11 and IL4 in kidney tissues from wild-type and Fabry mice (Fig. 4). Compared with the wild-type tissues, the expression levels of COL15A1, IL4 and MYH11 were significantly upregulated in the Fabry mouse kidney tissues. No significant upregulation in the expression of FOXP2 was observed in the Fabry mouse kidney (P=0.059).

Bottom Line: The majority of the biological functions of the regulated genes were associated with fibrogenesis or epithelial‑mesenchymal transition (EMT).In the HK‑2 cells, the ADAMTS6, BEST1, IL4, and MYH11 genes were upregulated.The gene expression profiles in kidney cells following the addition of Gb3 or lyso‑Gb3 revealed substrate‑specific and cell‑specific patterns.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 158‑710, Republic of Korea.

ABSTRACT
Fabry disease is an X-linked lysosomal storage disorder caused by mutations in the gene encoding the α-galactosidase A (α-Gal A) lysosomal enzyme, which results in globotriaosylceramide (Gb3) storage in vascular endothelial cells and different cell types throughout the body. Involvement of the kidney and heart is life threatening, and fibrosis of these organs is considered to be involved in the pathogenesis of Fabry disease. An increased concentration of deacylated Gb3 (lyso‑Gb3) in the plasma of symptomatic patients has also been suggested as a causative molecular event. To elucidate the molecular mechanisms involved in renal fibrosis in Fabry disease, the present analyzed the changes in global gene expression prior to and following Gb3 or lyso‑Gb3 treatment in two types of kidney cell lines, human proximal renal tubular epithelial (HK‑2) and mouse renal glomerular mesangial (SV40 MES 13) cells. Gb3 and lyso‑Gb3 treatment regulated the expression of 199 and 328 genes in each cell type, demonstrating a >2.0‑fold change. The majority of the biological functions of the regulated genes were associated with fibrogenesis or epithelial‑mesenchymal transition (EMT). The gene expression patterns of sphingolipid‑treated HK‑2 cells were distinguishable from the patterns in the SV40 MES 13 cells. Several genes associated with the EMT were selected and evaluated further in kidney cells and in Fabry mouse kidney tissues. In the SV40 MES 13 cells, the DLL1, F8, and HOXA11 genes were downregulated, and FOXP2 was upregulated by treatment with Gb3 or lyso‑Gb3. In the HK‑2 cells, the ADAMTS6, BEST1, IL4, and MYH11 genes were upregulated. Upregulation of the FOXP2, COL15A1, IL4, and MYH11 genes was also observed in the Fabry mouse kidney tissues. The gene expression profiles in kidney cells following the addition of Gb3 or lyso‑Gb3 revealed substrate‑specific and cell‑specific patterns. These findings suggested that Gb3 and lyso‑Gb3 lead to renal fibrosis in Fabry disease through different biochemical modulations.

Show MeSH
Related in: MedlinePlus