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HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

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HOXB7-S3 inhibits MCF-7 cell invasion. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. siRNA was transfected into MCF-7 cells for (A) 24 and (B) 48 h for the invasion assay. The number of migrated cells was significantly lower in the S3 group compared with in the Con-B group and Sn group at (C) 24 (**P<0.01) and (D) 48 h (***P<0.001). Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7.
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f8-mmr-12-04-4901: HOXB7-S3 inhibits MCF-7 cell invasion. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. siRNA was transfected into MCF-7 cells for (A) 24 and (B) 48 h for the invasion assay. The number of migrated cells was significantly lower in the S3 group compared with in the Con-B group and Sn group at (C) 24 (**P<0.01) and (D) 48 h (***P<0.001). Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7.

Mentions: To determine the effect of HOXB7-S3 on cell proliferation in MCF-7 breast cancer cells, the number of cells that had migrated through the Matrigel membrane was measured at 24 and 48 h post-transfection of MCF-7 cells with HOXB7-S3 by Transwell assay. As shown in Fig. 8A and B, the number of migrated cells in the S3 group was markedly reduced compared with in the Sn and Con-B groups. The number of migrated cells in the S3, Con-B and Sn groups 24 h post-transfection was 31±3.63, 46±1.87 and 47±2.27, respectively. The difference was statistically significant (**P<0.01; Fig. 8C). At 48 h post-transfection, the number of migrated cells in the S3 group was 75±3.49, which was apparently reduced compared with 125±7.18 in the Con-B group and 121±3.25 in the Sn group (Fig. 8B). The difference was statistically significant (***P<0.001; Fig. 8D). This suggested that HOXB7-S3 markedly inhibited the invasiveness of MCF-7 cells.


HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

HOXB7-S3 inhibits MCF-7 cell invasion. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. siRNA was transfected into MCF-7 cells for (A) 24 and (B) 48 h for the invasion assay. The number of migrated cells was significantly lower in the S3 group compared with in the Con-B group and Sn group at (C) 24 (**P<0.01) and (D) 48 h (***P<0.001). Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581815&req=5

f8-mmr-12-04-4901: HOXB7-S3 inhibits MCF-7 cell invasion. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. siRNA was transfected into MCF-7 cells for (A) 24 and (B) 48 h for the invasion assay. The number of migrated cells was significantly lower in the S3 group compared with in the Con-B group and Sn group at (C) 24 (**P<0.01) and (D) 48 h (***P<0.001). Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7.
Mentions: To determine the effect of HOXB7-S3 on cell proliferation in MCF-7 breast cancer cells, the number of cells that had migrated through the Matrigel membrane was measured at 24 and 48 h post-transfection of MCF-7 cells with HOXB7-S3 by Transwell assay. As shown in Fig. 8A and B, the number of migrated cells in the S3 group was markedly reduced compared with in the Sn and Con-B groups. The number of migrated cells in the S3, Con-B and Sn groups 24 h post-transfection was 31±3.63, 46±1.87 and 47±2.27, respectively. The difference was statistically significant (**P<0.01; Fig. 8C). At 48 h post-transfection, the number of migrated cells in the S3 group was 75±3.49, which was apparently reduced compared with 125±7.18 in the Con-B group and 121±3.25 in the Sn group (Fig. 8B). The difference was statistically significant (***P<0.001; Fig. 8D). This suggested that HOXB7-S3 markedly inhibited the invasiveness of MCF-7 cells.

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

Show MeSH
Related in: MedlinePlus