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HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

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Apoptotic rate following transfection for 48 h in MCF-7 cells. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. The apoptotic rate in S3 groups was the highest compared with that in Con-B groups and Sn groups (**P<0.01). (A) Early apoptotic cells are defined as Annexin V+/PI−, whereas late apoptotic cells are Annexin V+/PI+. Data are representative results for one of the three replicates. (B) Percentage of Annexin V+/PI− MCF-7 cells following transfection with S3 for 48 h. HOXB7-S3 effectively enhanced MCF-7 cell apoptosis. Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7; PI, propidium iodide; siRNA, small interfering RNA.
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f7-mmr-12-04-4901: Apoptotic rate following transfection for 48 h in MCF-7 cells. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. The apoptotic rate in S3 groups was the highest compared with that in Con-B groups and Sn groups (**P<0.01). (A) Early apoptotic cells are defined as Annexin V+/PI−, whereas late apoptotic cells are Annexin V+/PI+. Data are representative results for one of the three replicates. (B) Percentage of Annexin V+/PI− MCF-7 cells following transfection with S3 for 48 h. HOXB7-S3 effectively enhanced MCF-7 cell apoptosis. Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7; PI, propidium iodide; siRNA, small interfering RNA.

Mentions: To detect whether the effect of HOXB7-S3 on cell proliferation in MCF-7 breast cancer cells occurred by apoptosis, the percentage of early-stage apoptotic cells and late-stage apoptotic cells was measured in three groups by FCM. At 48 h post-transfection of siRNA, the total number of apoptotic cells (12.70±1.75%) was markedly increased in the S3 group, compared with 7.83±0.47% in the Con-B group and 6.46±0.49% in the Sn group, respectively (Fig. 7). The differences between the three groups were statistically significant (**P<0.01). These results indicated that HOXB7-S3 enhanced MCF-7 cell apoptosis.


HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Apoptotic rate following transfection for 48 h in MCF-7 cells. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. The apoptotic rate in S3 groups was the highest compared with that in Con-B groups and Sn groups (**P<0.01). (A) Early apoptotic cells are defined as Annexin V+/PI−, whereas late apoptotic cells are Annexin V+/PI+. Data are representative results for one of the three replicates. (B) Percentage of Annexin V+/PI− MCF-7 cells following transfection with S3 for 48 h. HOXB7-S3 effectively enhanced MCF-7 cell apoptosis. Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7; PI, propidium iodide; siRNA, small interfering RNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581815&req=5

f7-mmr-12-04-4901: Apoptotic rate following transfection for 48 h in MCF-7 cells. Con-B group, blank control group; Sn group, negative control siRNA; S3 group, S3 transfection group. The apoptotic rate in S3 groups was the highest compared with that in Con-B groups and Sn groups (**P<0.01). (A) Early apoptotic cells are defined as Annexin V+/PI−, whereas late apoptotic cells are Annexin V+/PI+. Data are representative results for one of the three replicates. (B) Percentage of Annexin V+/PI− MCF-7 cells following transfection with S3 for 48 h. HOXB7-S3 effectively enhanced MCF-7 cell apoptosis. Data are the average of three independent experiments each containing three replicates. HOXB7, homeobox B7; PI, propidium iodide; siRNA, small interfering RNA.
Mentions: To detect whether the effect of HOXB7-S3 on cell proliferation in MCF-7 breast cancer cells occurred by apoptosis, the percentage of early-stage apoptotic cells and late-stage apoptotic cells was measured in three groups by FCM. At 48 h post-transfection of siRNA, the total number of apoptotic cells (12.70±1.75%) was markedly increased in the S3 group, compared with 7.83±0.47% in the Con-B group and 6.46±0.49% in the Sn group, respectively (Fig. 7). The differences between the three groups were statistically significant (**P<0.01). These results indicated that HOXB7-S3 enhanced MCF-7 cell apoptosis.

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

Show MeSH
Related in: MedlinePlus