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HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

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Protein expression of HOXB7 following transfection for 72 h: Con-B group, blank control group; Sn group, negative control siRNA; S1 group, S1 transfection group; S2 group, S2 transfection group and S3 group, S3 transfection group. (A) The protein expression level of HOXB7 was measured by western blotting. (B) The optical density for target protein is shown as a proportion of GAPDH optical density. Cells in the S3 group had the lowest HOXB7 protein expression level (***P<0.001, compared with the Con B and Sn groups). HOXB7, homeobox B7.
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f5-mmr-12-04-4901: Protein expression of HOXB7 following transfection for 72 h: Con-B group, blank control group; Sn group, negative control siRNA; S1 group, S1 transfection group; S2 group, S2 transfection group and S3 group, S3 transfection group. (A) The protein expression level of HOXB7 was measured by western blotting. (B) The optical density for target protein is shown as a proportion of GAPDH optical density. Cells in the S3 group had the lowest HOXB7 protein expression level (***P<0.001, compared with the Con B and Sn groups). HOXB7, homeobox B7.

Mentions: To further determine the interference efficiency of HOXB7-siRNA on MCF-7 cells, western blotting was used following transfection for 72 h. The results demonstrated that HOXB7 protein was overexpressed in the Con-B and Sn groups following transfection and no differences were observed between the two groups (P>0.05). However, the expression of HOXB7 protein in the S1, S2 and S3 groups was significantly downregulated compared with the Con-B and Sn groups with interference efficiencies of 53.88±0.0001, 65.22±0.004 and 65.25±0.001%, respectively. The differences were all statistically significant (***P<0.001; Fig. 5). The HOXB7 protein expression level was the lowest in the S3 group and differed markedly from that in the Con-B and Sn groups (***P<0.001; Fig. 5). Combined with the result in Fig. 4, S3 had the maximum inhibition efficiency of HOXB7 and therefore S3 was used in the following experiments.


HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Protein expression of HOXB7 following transfection for 72 h: Con-B group, blank control group; Sn group, negative control siRNA; S1 group, S1 transfection group; S2 group, S2 transfection group and S3 group, S3 transfection group. (A) The protein expression level of HOXB7 was measured by western blotting. (B) The optical density for target protein is shown as a proportion of GAPDH optical density. Cells in the S3 group had the lowest HOXB7 protein expression level (***P<0.001, compared with the Con B and Sn groups). HOXB7, homeobox B7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581815&req=5

f5-mmr-12-04-4901: Protein expression of HOXB7 following transfection for 72 h: Con-B group, blank control group; Sn group, negative control siRNA; S1 group, S1 transfection group; S2 group, S2 transfection group and S3 group, S3 transfection group. (A) The protein expression level of HOXB7 was measured by western blotting. (B) The optical density for target protein is shown as a proportion of GAPDH optical density. Cells in the S3 group had the lowest HOXB7 protein expression level (***P<0.001, compared with the Con B and Sn groups). HOXB7, homeobox B7.
Mentions: To further determine the interference efficiency of HOXB7-siRNA on MCF-7 cells, western blotting was used following transfection for 72 h. The results demonstrated that HOXB7 protein was overexpressed in the Con-B and Sn groups following transfection and no differences were observed between the two groups (P>0.05). However, the expression of HOXB7 protein in the S1, S2 and S3 groups was significantly downregulated compared with the Con-B and Sn groups with interference efficiencies of 53.88±0.0001, 65.22±0.004 and 65.25±0.001%, respectively. The differences were all statistically significant (***P<0.001; Fig. 5). The HOXB7 protein expression level was the lowest in the S3 group and differed markedly from that in the Con-B and Sn groups (***P<0.001; Fig. 5). Combined with the result in Fig. 4, S3 had the maximum inhibition efficiency of HOXB7 and therefore S3 was used in the following experiments.

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

Show MeSH
Related in: MedlinePlus